CdTe-GSH as luminescent biomarker for labeling the larvicidal action of WSMoL lectin in Aedes aegypti larvae

Arruda, Camila C.L.; Freitas, Denilson V.; Seabra, Maria Aparecida Barreto Lopes; Xavier, Francisco Humberto; Figueiredo, Regina Célia Bressan Queiroz de; Napoleão, Thiago Henrique; Paiva, Patrícia Maria Guedes; Navarro, Daniela; Navarro, Marcelo

doi:10.1016/j.colsurfb.2019.110672

Cited by 9 publications

(2 citation statements)

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“…25 It has been reported that water-soluble Moringa oleifera lectin (WSMoL) is localized in the intestinal nutrients of Aedes aegypti larvae by combining WSMoL with glutathionemodified CdTe QDS to form a fluorescent bioconjugate. 26 Drug Affinity Responsive Target Stability (DARTS) is a common method for finding the targets of chemical compounds. It does not modify small molecules and retains its structure to bind directly to targets.…”

Section: T H Imentioning

confidence: 99%

“…Yan et al used genomics approaches to successfully identify that natural herbicide Aspterric Acid inhibited plant growth by specifically inhibiting dihydroxyacid dehydrase, involved in the synthesis of branched-chain amino acids . It has been reported that water-soluble Moringa oleifera lectin (WSMoL) is localized in the intestinal nutrients of Aedes aegypti larvae by combining WSMoL with glutathione-modified CdTe QDS to form a fluorescent bioconjugate . Drug Affinity Responsive Target Stability (DARTS) is a common method for finding the targets of chemical compounds.…”

Section: Introductionmentioning

confidence: 99%

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Herbicidal Active Compound Ferulic Acid Ethyl Ester Affects Fatty Acid Synthesis by Targeting the 3-Ketoacyl-Acyl Carrier Protein Synthase I (KAS I)

Jia

et al. 2023

J. Agric. Food Chem.

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Exploring new herbicide targets based on natural product derivatives is an important research aspect for the generation of innovative pesticides. Ferulic acid ethyl ester (FAEE), a natural product derivative from ferulic acid, has significant herbicidal activity mainly by inhibiting the normal growth of weed seedling roots. However, the FAEE target protein underlying its herbicidal activity has not been identified. In this study, we synthesized an FAEE probe to locate its site of action. We discovered that FAEE entry point was via the root tips. Fourteen major binding proteins were identified using Drug affinity responsive target stability (DARTS) combined with LC-MS/MS, which included 3-ketoacyl-acyl carrier protein synthase I (KAS I) and phenylalanine ammonia-lyase I (PAL I). The KAS I and PAL I proteins/genes expression was changed significantly after exposure to FAEE, as evidenced by combined transcriptomic and proteomic analysis. A molecular docking assay indicated that KAS I and FAEE had a strong binding ability. Combined with previous studies on FAEE mechanism of action, and based on our results, we conclude that FAEE targeting KAS I lead to the blockage of the fatty acid synthesis pathway and result in plant death.

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Section: T H Imentioning

confidence: 99%