volume 87, issue 16, P6358-6362 1990
DOI: 10.1073/pnas.87.16.6358
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Abstract: ABSTRACTA Agtll cDNA library was constructed from poly(U)-Sepharose-selected Entamoeba histolyica trophozoite RNA in order to clone and identify surface antigens. The library was screened with rabbit polyclonal anti-E. histolytica serum. A 700-base-pair cDNA insert was isolated and the nucleotide sequence was determined. The deduced amino acid sequence of the cDNA revealed a cysteine-rich protein. DNA hybridizations showed that the gene was specific to E. histolytica since the cDNA probe reacted with DNA from…

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