CDMP‐2 injection improves early tendon healing in a rabbit model for surgical repair

Abstract

This study examines the hypothesis that cartilage-derived morphogenic protein-2 (CDMP-2) can improve tendon healing after surgical repair. We have previously found improved tendon healing by applying CDMP-2 in models for conservative treatment with mechanically loaded Achilles tendon defects in rats and rabbits. In this study, the patellar tendon was unloaded by patello- tibial cerclage and cut transversely in 40 rabbits. Two hours post-operative, the rabbits received a dose of 20 microg of CDMP-2 or vehicle injected into the hematoma. Specimens were harvested after 14 and 28 days and evaluated by biomechanical testing, radiography and histology. At 14 days, CDMP-2 caused a 65% increase in force at failure, a 50% increase in ultimate stress and a 57% increase in stiffness, as compared with controls. There was no effect on callus size. At 28 days, no differences between the treatment groups could be demonstrated. No bone or cartilage was found in any tendon or regenerated tissue at any time point. Thus, early tendon repair can be stimulated by CDMP-2 in an unloaded model. These results suggest that CDMP-2 might be of interest for clinical use as a complement to surgical treatment of tendon ruptures.

The effect of administration of double stranded MicroRNA-210 on acceleration of Achilles tendon healing in a rat model

“…Vascular supply plays an important role in primary tendon healing [26,27]. Tendon healing undergoes three phase, including an inflammatory phase, a proliferative phase, and a remodeling phase [28].…”

Physical activity modulates nerve plasticity and stimulates repair after achilles tendon rupture

“…As for tendon healing, most studies have been confined to micro-and macromorphology based on histology, ultrasound, and MRI, [29][30][31][32] in addition to analysis of mechanical strength. [32][33][34] In this study, we chose to measure the mediolateral diameter of organized collagen at the rupture site.…”

“…[32][33][34] In this study, we chose to measure the mediolateral diameter of organized collagen at the rupture site. We are not aware of any other quantitative way of assessing healing histologically.…”

Stimulation of tendon repair: mechanical loading, GDFs and platelets. A mini-review

“…A single injection of 20 µg CDMP into the haematoma increased the strength by PDGF platelet-derived growth factor, MCL medial collateral ligament, TGF transforming growth factor, FGF fibroblast growth factor, IHC immunohistochemistry, VEGF vascular endothelial growth factor, CDMP cartilage-derived morphogenetic protein, IGF insulin-like growth factor, OP osteogenic protein more than 65% [26]. For the first time, this occurred without an increase in callus transverse area; the increase in force at failure at mechanical testing was entirely due to improved material properties.…”