2017
DOI: 10.18632/aging.101173
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Abstract: Patients with chronic kidney disease (CKD) display a progeric vascular phenotype linked to apoptosis, cellular senescence and osteogenic transformation. This has proven intractable to modelling appropriately in model organisms. We have therefore investigated this directly in man, using for the first time validated cellular biomarkers of ageing (CDKN2A/p16INK4a, SA-β-Gal) in arterial biopsies from 61 CKD patients undergoing living donor renal transplantation. We demonstrate that in the uremic milieu, increased … Show more

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Cited by 44 publications
(36 citation statements)
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References 45 publications
(49 reference statements)
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“…While association between CRP and vascular calcification in CKD [28] has been shown, and CRP seems related to progression of carotid stenosis [29], a positive association to carotid calcification has not been shown before. Possibly, the moderate association found in our study reflects residual long-term systemic inflammation or increased vascular senescence in end-stage atherosclerosis, similarly as previously shown in CKD [9].…”
Section: Discussionsupporting
confidence: 87%
See 1 more Smart Citation
“…While association between CRP and vascular calcification in CKD [28] has been shown, and CRP seems related to progression of carotid stenosis [29], a positive association to carotid calcification has not been shown before. Possibly, the moderate association found in our study reflects residual long-term systemic inflammation or increased vascular senescence in end-stage atherosclerosis, similarly as previously shown in CKD [9].…”
Section: Discussionsupporting
confidence: 87%
“…Calcification is a prominent feature of end-stage atherosclerosis, but it is also typical for the artery media of patients with chronic kidney disease (CKD) [9,10]. Vascular calcification can be influenced by altered Ca and phosphate homeostasis, loss of mineralization inhibitors and dysfunction of natural regulators in bone metabolism such as osteopontin, osteoprotegerin, matrix-gla protein, fetuin-A and receptor activator of NF-κB.…”
Section: Introductionmentioning
confidence: 99%
“…Inferior epigastric artery samples collected at LD-RTx (n = 41) were incubated overnight in AllProtect Tissue Reagent (Qiagen, Hilden, Germany) and subsequently stored at −70 °C. RNA was isolated from arteries using TRIzol Reagent and MGP mRNA levels were analysed with TaqMan chemistry (Thermo Fisher Scientific, Waltham, MA USA) as previously described 37 .…”
Section: Ucmgp Immunohistochemical Staining After Deparaffinization mentioning
confidence: 99%
“…Isolation of RNA from epigastric artery and subsequent cDNA preparation were executed as described before. 3 Gene expression was analyzed using a TaqMan Low Density Array on a QuantStudioTM 7 Flex Real-Time PCR system (LifeTechnologies, Carlsbad, CA, USA) according to standard protocol. Amplification profiles for each sample and target were normalized against endogenous control genes (mitochondrially encoded 16S RNA, MT-RNR2; TATA box binding protein, TBP; and glyceraldehyde-3-phosphate dehydrogenase, GAPDH).…”
Section: Patient Population and Taqman Low Density Arraymentioning
confidence: 99%
“…Medial calcification is highly prevalent in disorders characterized with premature vascular ageing, such as chronic kidney disease (CKD), and diabetes mellitus, and contributes to increased cardiovascular morbidity and mortality. [1][2][3][4] Under physiological conditions VSMCs are responsible of maintaining a variable contractile tone of the arteries. To achieve this, they express a wide variety of contractile proteins such as alpha smooth muscle actin (ACTA2), and Calponin (CNN1).…”
Section: Introductionmentioning
confidence: 99%