CD9+ Regulatory B Cells Induce T Cell Apoptosis via IL-10 and Are Reduced in Severe Asthmatic Patients

Brosseau, Carole; Durand, M.; Colas, Luc; Durand, Eugénie; Foureau, Aurore; Cheminant, Marie‐Aude; Bouchaud, Grégory; Castan, Laure; Klein, Martin; Magnan, A.; Brouard, Sophie

doi:10.3389/fimmu.2018.03034

Cited by 46 publications

(32 citation statements)

References 59 publications

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“…Major Cell Origin CD81 B cells [18][19][20][21], T cells [22], Natural Killer (NK) cells [23] CD9 B cells [24,25], T cells [26], NK cells, monocytes, macrophages, DCs [27] CD29 Adipose-derived stem cells [28], mesenchymal stem cells [29,30] CD63 T cells [31], NK cells [23], platelets [32], basophils [33] CD8 Cytotoxic T cells [34][35][36]…”

Section: Markersmentioning

confidence: 99%

Immune cell extracellular vesicles and their mitochondrial content decline with ageing

2020

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Background: Although the mechanisms of action are not fully understood, extracellular vesicles (EVs) have emerged as key indicators and effectors of immune function. Characterizing circulating EVs associated with stem and immune cells across the lifespan of healthy individuals could aid an understanding of immunosenescence, a process of age-related decline of cells in both adaptive and innate immune systems. Results: Using high resolution multicolor flow cytometry, we identified three major subsets of EVs of varying sizes in healthy control (HC) plasma. Multiple plasma EVs associated with immune cells declined with ageing in HCs. In addition, we observed age-associated declines of respiring mitochondria cargo in EVs of several types of immune cells, suggesting that these parent cells may experience a decline in mitophagy or a mitochondrial dysfunctioninduced immunosenescence. By contrast, the number of CD34 + hematopoietic stem cell-associated EVs were high and carried respiring mitochondria, which did not decline with age. Conclusion: As demonstrated here, multicolor flow cytometry simultaneously measures plasma EV size, surface markers and cargo that reflect biological processes of specific cell types. The distinct surface markers and cytokine cargo of plasma EVs suggest that they may carry different bio-messages and originate by different biogenesis pathways.

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Section: Markersmentioning

confidence: 99%

Immune cell extracellular vesicles and their mitochondrial content decline with ageing

2020

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“…Other surface receptors expressed on B cells, and up-regulated upon their activation, have been associated with immunosuppressive B cells. The tetraspanin CD9, which is expressed by marginal zone B cells, transitional B cells, B1 cells, and plasma cells, is such a marker [19], [20], [21]. CD9 expression can be acquired by B cells upon activation [19].…”

Section: B Cell Subsets With Suppressive Functions In Adoptive Transfmentioning

confidence: 99%

Regulatory functions of B cells and regulatory plasma cells

Fillatreau

2019

Biomedical Journal

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B cells critically contribute to health through the production of antibodies that provide a vital line of defence against infectious agents. In addition, B cells are known to play an integrative role in immunity, acting as crucial antigen-presenting cells for T cells, and being an important source of cytokines that can target multiple cell types including stromal cells, innate cells, and adaptive lymphocytes. This review focuses on the role of B cells as negative regulators of immunity through the production of interleukin-10 (IL-10) in autoimmune, infectious, and malignant diseases. It discusses the phenotypes of the B cell subsets most competent to produce IL-10 in vitro and to exert suppressive functions in vivo upon adoptive transfer in recipient mice, the signals and transcription factors regulating IL-10 expression in B cells, and the recent identification of plasmocytes, including short-lived plasmablasts and long-lived plasma cells, as an important source of IL-10 in secondary lymphoid organs and inflamed tissues in vivo during mouse and human diseases. With our increasing knowledge of this non-canonical B cell function a coherent framework starts emerging that will help monitoring and targeting this B cell function in health and disease.

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“…The interaction between apoptosis and autophagy is worth noting: autophagy-related proteins interact with apoptosis-related proteins and some proteins can have effects on both apoptosis and autophagy [ 25 , 26 ]. In addition, this interaction plays a key role in airway remodeling through maintaining cellular homeostasis and mediating cell death process [ 27 ], indicating that the dysregulated apoptosis and autophagy are involved in asthmatic pathogenesis [ 28 , 29 ]. This means that the regulation of apoptosis and autophagy may be one of mechanisms of improving airway remodeling in asthma.…”

Section: Introductionmentioning

confidence: 99%

Combined Extracts of Epimedii Folium and Ligustri Lucidi Fructus with Budesonide Attenuate Airway Remodeling in the Asthmatic Rats by Regulating Apoptosis and Autophagy

Tang

Yingying

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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This study aimed to investigate the effects of the coadministration of budesonide (Bud) and the extracts of Epimedii Folium and Ligustri Lucidi Fructus (EEL) on regulating apoptosis and autophagy in asthmatic rats. Forty Sprague-Dawley rats were divided randomly into five groups (8 rats in each group): normal control (control), asthma model (asthma), Bud (1 mg Bud suspension in 50 ml sterile physiological saline for 30 min), EEL (100 mg/kg EEL), and group of coadministration of Bud and EEL (Bud&EEL, 100 mg/kg EEL plus Bud by nebulized inhalation for 30 min). Rats were sensitized and challenged with ovalbumin for 7 weeks and treated with corresponding drug for 4 weeks. We anesthetized all rats with 25% ethyl carbamate (4 ml/kg) and took lung tissues and BALF after final ovalbumin challenge to observe the lung histopathology and morphometry; apoptosis in BALF and lung tissue; protein expressions of Ki-67, α-SMA, cleaved Caspase-3, p-mTOR, and LC3; and protein and mRNA expressions of Bax, Bcl-2, Caspase-3, P53, mTOR, and Beclin-1. Results showed that Bud&EEL could alleviate airway remodeling, inhibit cell proliferation and autophagy in lung tissue, and promote apoptosis in BALF and lung tissue in ovalbumin-induced asthma rats through downregulating the protein expressions of α-SMA and Ki-67, the protein ratio of LC3-II/LC3-I and Bcl-2/Bax, and the protein and mRNA expressions of Bcl-2 and Beclin-1, while upregulating the protein expressions of cleaved Caspase-3 and p-mTOR, and the protein and mRNA expressions of Bax, Caspase-3, P53, and mTOR. Bud&EEL had better effects than single-use Bud on improving airway remodeling, promoting apoptosis, and regulating the expressions of autophagy- and apoptosis-related proteins. This study suggested that the effects of coadministration of EEL and Bud on regulating apoptosis and autophagy were better than those of single-use Bud treatment, and that might be the mechanism of attenuating airway remodeling, providing an alternative therapy for asthma.

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CD9+ Regulatory B Cells Induce T Cell Apoptosis via IL-10 and Are Reduced in Severe Asthmatic Patients

Cited by 46 publications

References 59 publications

Immune cell extracellular vesicles and their mitochondrial content decline with ageing

Immune cell extracellular vesicles and their mitochondrial content decline with ageing

Regulatory functions of B cells and regulatory plasma cells

Combined Extracts of Epimedii Folium and Ligustri Lucidi Fructus with Budesonide Attenuate Airway Remodeling in the Asthmatic Rats by Regulating Apoptosis and Autophagy

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