CD48 expression on leukocytes in infectious diseases: Flow cytometric analysis of surface antigen

Katsuura, Michihiko; Shimizu, Yasuyuki; Akiba, Kaori; Kanazawa, Chikako; Mitsui, Tetsuo; Sendo, Dai; Kawakami, Takako; Hayasaka, Kiyoshi; Yokoyama, Shinkichi

doi:10.1111/j.1442-200x.1998.tb01994.x

Cited by 12 publications

(8 citation statements)

References 38 publications

(1 reference statement)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…CD48 has been reported to be elevated in the serum and on the surface of hemopoietic cells from patients with leukemia and infectious diseases (12,14). Assessment of both peripheral blood and nasal polyp eosinophils from atopic asthmatics demonstrated that CD48 was indeed elevated in patients with allergic disease compared with nonatopic controls.…”

Section: Discussionmentioning

confidence: 98%

See 1 more Smart Citation

CD48 Is an Allergen and IL-3-Induced Activation Molecule on Eosinophils

Munitz

Bachelet

Eliashar

et al. 2006

The Journal of Immunology

View full text Add to dashboard Cite

Eosinophils are involved in a variety of allergic, parasitic, malignant, and idiopathic disorders by releasing a variety of factors including specific granule proteins, lipid mediators, and proinflammatory and immunoregulatory cytokines and chemokines. In addition, they interact with various cell types in the inflamed tissue. Yet, the mechanism of eosinophil activation is still poorly understood. Recently, we described the expression and function of the CD2-subfamily of receptors and especially 2B4 on human eosinophils. In this study we focus on CD48, the high-affinity ligand of 2B4. CD48 is a GPI-anchored protein involved in cellular activation, costimulation, and adhesion, but has not been studied on eosinophils. We demonstrate that human eosinophils from atopic asthmatics display enhanced levels of CD48 expression and that IL-3 up-regulates CD48 expression. Furthermore, cross-linking CD48 on human eosinophils triggers release of eosinophil granule proteins. Assessment of CD48 expression in a murine model of experimental asthma revealed that CD48 is induced by allergen challenge and partially regulated by IL-3. Additionally, anti-IL-3 reduces CD48 expression and the degree of airway inflammation. Thus, CD48 is an IL-3-induced activating receptor on eosinophils, likely involved in promoting allergic inflammation.

show abstract

Section: Discussionmentioning

confidence: 98%

“…Interestingly, CD48 expression is increased in several infectious diseases, including varicella, measles, rubella, mononucleosis, streptococcus tonsillitis, sepsis, and appendicitis (14).…”

mentioning

confidence: 99%

CD48 Is an Allergen and IL-3-Induced Activation Molecule on Eosinophils

Munitz

Bachelet

Eliashar

et al. 2006

The Journal of Immunology

View full text Add to dashboard Cite

show abstract

“…In fact, the only hematopoietic cell populations in mice that distinctly lack CD48 expression in mice are neutrophils and a subset of long-term hematopoietic stem cells (LT-HSCs) [59, 60]. However, in humans CD48 is also found on neutrophils and other HSC types [61, 62]. …”

Section: Cd48 Structure and Expressionmentioning

confidence: 99%

“…Exposure to cytokines such as IFNα, IFNβ and IFNγ can increase CD48 expression on human peripheral blood mononuclear cells [63]. Monocytes and lymphocytes have elevated CD48 expression in patients with viral and bacterial infections [61]. Patients with EBV infection or arthritis exhibit elevated levels of soluble CD48 in the serum [52].…”

Section: Cd48 Structure and Expressionmentioning

confidence: 99%

Roles of CD48 in regulating immunity and tolerance

McArdel

Terhorst

Sharpe

2016

Clinical Immunology

152

134

View full text Add to dashboard Cite

CD48, a member of the signaling lymphocyte activation molecule family, participates in adhesion and activation of immune cells. Although constitutively expressed on most hematopoietic cells, CD48 is upregulated on subsets of activated cells. CD48 can have activating roles on T cells, antigen presenting cells and granulocytes, by binding to CD2 or bacterial FimH, and through cell intrinsic effects. Interactions between CD48 and its high affinity ligand CD244 are more complex, with both stimulatory and inhibitory outcomes. CD244:CD48 interactions regulate target cell lysis by NK cells and CTLs, which are important for viral clearance and regulation of effector/memory T cell generation and survival. Here we review roles of CD48 in infection, tolerance, autoimmunity, and allergy, as well as the tools used to investigate this receptor. We discuss stimulatory and regulatory roles for CD48, its potential as a therapeutic target in human disease, and current challenges to investigation of this immunoregulatory receptor.

show abstract

“…Clinically, membrane and soluble CD48 expression increases under inflammatory conditions and has been shown to be elevated in patients with systemic inflammatory disorders, hematopoietic malignancies, infections, and allergic diseases [ 3 , 8 , 9 , 11 – 13 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Soluble CD48 Levels in Patients with Allergic and Nonallergic Asthma in Relation to Markers of Type 2 and Non-Type 2 Immunity: An Observational Study

Breuer

Gangwar

Seaf

et al. 2018

Journal of Immunology Research

View full text Add to dashboard Cite

CD48 is a costimulatory receptor associated with human asthma. We aimed to assess the significance of the soluble form of CD48 (sCD48) in allergic and nonallergic asthma. Volunteer patients completed an asthma and allergy questionnaire, spirometry, methacholine challenge test, a common allergen skin prick test, and a complete blood count. sCD48, IgE, IL5, IL17A, IL33, and IFNγ were quantitated in serum by ELISA. Asthma was defined as positive methacholine challenge test or a 15% increase in FEV1 post bronchodilator in symptomatic individuals. Allergy was defined as positive skin test or IgE levels > 200 IU/l in symptomatic individuals. 137 individuals participated in the study: 82 (60%) were diagnosed with asthma of which 53 (64%) was allergic asthma. sCD48 levels were significantly elevated in patients with nonallergic asthma compared to control and to the allergic asthma cohort (median (IQR) pg/ml, 1487 (1338–1758) vs. 1308 (1070–1581), p < 0.01, and 1336 (1129–1591), p = 0.02, respectively). IL17A, IL33, and IFNγ levels were significantly elevated in allergic and nonallergic asthmatics when compared to control. No correlation was found between sCD48 level and other disease markers. sCD48 is elevated in nonallergic asthma. Additional studies are required for understanding the role of sCD48 in airway disease.

show abstract

CD48 expression on leukocytes in infectious diseases: Flow cytometric analysis of surface antigen

Cited by 12 publications

References 38 publications

CD48 Is an Allergen and IL-3-Induced Activation Molecule on Eosinophils

CD48 Is an Allergen and IL-3-Induced Activation Molecule on Eosinophils

Roles of CD48 in regulating immunity and tolerance

Evaluation of Soluble CD48 Levels in Patients with Allergic and Nonallergic Asthma in Relation to Markers of Type 2 and Non-Type 2 Immunity: An Observational Study

Contact Info

Product

Resources

About