CD34+ progenitors are reproducibly recovered in thawed umbilical grafts, and positively influence haematopoietic reconstitution after transplantation

Lemarié, Claude; Esterni, Benjamin; Calmels, Boris; Dazey, Bernard; Lapierre, Valérie; Lecchi, Lucilla; Meyer, Anne-Louise; Réa, Delphine; Thuret, Isabelle; Chambost, Hérvè; Curtillet, Catherine; Chabannon, Christian; Gautier, Michel

doi:10.1038/sj.bmt.1705618

Cited by 24 publications

(11 citation statements)

References 34 publications

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“…However, there are renal adverse events associated with the use of HES , which leads to limitation in the use and availability of this product similar to dextran 40. Other authors also report CD34 + viable cells recovery around 51·5% , 60% and other around 80% similar to our results with the experimental solution. There are consistent evidence that the number and viability of CD45 and CD34 + cells have a direct correlation on the graft success rate , indicating the relevance of optimizing CD34 + and CD45 viable cells recovery after cryopreservation and thawing.…”

Section: Discussionsupporting

confidence: 92%

Evaluation of DMSO dextrose as a suitable alternative for DMSO dextran in cord blood cryopreservation

Raffo¹,

Tito²,

Pes³

et al. 2019

Vox Sanguinis

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Background and Objectives Umbilical cord blood is considered an alternative source of hematopoietic stem cells. Standard banking procedures use 50/55% DMSO in dextran 40 for cryopreservation and dextran-based solutions for thawing, however, due to the potential risk of crystallization of dextran, dextran 40 approved for clinical use has become limited or unavailable. This affects cryopreservation and thawing procedures. Carbohydrates, in particular sucrose, trehalose and glucose, have been shown to be effective in reducing cell damage during dehydration and have cryoprotective potential. We aim to study a 50/55% DMSO in 5% dextrose cryopreservation solution as an alternative to DMSO dextran.Materials and Methods Eighteen samples were divided into two aliquots and cryopreserved, one using standard solution and the other with DMSO dextrose experimental solution. Both aliquots were thawed and diluted with PBS or saline. Total nucleated cells counts, 7-AAD viability of CD45 + cells and recovery of CD34 + viable cells were assessed on thawed samples and compared between pair of aliquots. ResultsNo differences were observed in the total nucleated cells recovery between cryopreservation solutions, however, higher viability and CD34 + viable cells recoveries were observed using the experimental solution.Conclusion Results showed that DMSO dextrose cryopreservation solution had better results than the standard solution when thawed in an isotonic solution. This indicates that DMSO dextrose is probably a better alternative for direct infusion or when dextran thawing solutions are unavailable. Viability of CD45 + cells and recovery of CD34 + viable cells have positive correlation with engraftment, highlighting the relevance of the optimization of the cryopreservation and thawing process.

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Section: Discussionsupporting

confidence: 92%

Evaluation of DMSO dextrose as a suitable alternative for DMSO dextran in cord blood cryopreservation

Raffo¹,

Tito²,

Pes³

et al. 2019

Vox Sanguinis

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“…These findings have been replicated in subsequent studies to varying degrees 2,4,9,29 . In a smaller number of studies, CD34+ cell dose has been shown to predict engraftment and survival, 5‐9 but the use of CD34+ is limited by standardization issues and CD34+ enumeration is not available for a significant portion of the existing banking inventory. In addition, the precryopreservation TNC and/or CD34+ content do not necessarily reflect the overall potency of the CBU after cryopreservation, storage, and thawing.…”

Section: Discussionmentioning

confidence: 83%

“…It is believed that a significant reason for this relates to the overall quality of the cord blood (CB) graft. Attempts at correlating other graft variables, for example, CD34+ cells or colony‐forming units (CFUs), with engraftment have shown superior predictive value in limited studies, 5‐12 but no single variable has a high enough correlation with engraftment or graft failure. Furthermore, individual CBU profiles may show discordant, rather than linear, correlations of one variable compared to another.…”

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confidence: 99%

The Cord Blood Apgar: a novel scoring system to optimize selection of banked cord blood grafts for transplantation (CME)

et al. 2011

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BACKGROUND Engraftment failure and delays, likely due to diminished cord blood unit (CBU) potency, remain major barriers to the overall success of unrelated umbilical cord blood transplantation (UCBT). To address this problem, we developed and retrospectively validated a novel scoring system, the Cord Blood Apgar (CBA), which is predictive of engraftment after UCBT. STUDY DESIGN AND METHODS In a single-center retrospective study, utilizing a database of 435 consecutive single cord myeloablative UCBTs performed between January 1, 2000, to December 31, 2008, precryopreservation and postthaw graft variables (total nucleated cell, CD34+, colony-forming units, mononuclear cell content, and volume) were initially correlated with neutrophil engraftment. Subsequently, based on the magnitude of hazard ratios (HRs) in univariate analysis, a weighted scoring system to predict CBU potency was developed using a randomly selected training data set and internally validated on the remaining data set. RESULTS The CBA assigns transplanted CBUs three scores: a precryopreservation score (PCS), a postthaw score (PTS), and a composite score (CS), which incorporates the PCS and PTS values. CBA-PCS scores, which could be used for initial unit selection, were predictive of neutrophil (CBA-PCS ≥ 7.75 vs. <7.75, HR 3.5; p < 0.0001) engraftment. Likewise, CBA-PTS and CS scores were strongly predictive of Day 42 neutrophil engraftment (CBA-PTS ≥ 9.5 vs. <9.5, HR 3.16, p < 0.0001; CBA-CS ≥ 17.75 vs. <17.75, HR 4.01, p < 0.0001). CONCLUSION The CBA is strongly predictive of engraftment after UCBT and shows promise for optimizing screening of CBU donors for transplantation. In the future, a segment could be assayed for the PTS score providing data to apply the CS for final CBU selection.

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“…Thrombocytopenia occurs in many hematological diseases and also after hematopoietic stem cell transplantation, intensive chemotherapy or radiotherapy (1)(2)(3)(4)(5)(6)(7). Repeated platelet transfusion increases the risk of alloimmunization, bacterial and viral infection, and transfusion reaction (8)(9)(10).…”

Section: Introductionmentioning

confidence: 99%

Radiation Sensitivities in the Terminal Stages of Megakaryocytic Maturation and Platelet Production

Monzen¹,

Osuda²,

Miyazaki³

et al. 2009

Radiation Research

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These studies examined the effects of X radiation and interleukin 3 (IL-3), which is an effective cytokine for the generation of megakaryocytopoiesis from X-irradiated hematopoietic stem/progenitor cells, on the terminal process of human megakaryocytopoiesis and thrombopoiesis. Mature megakaryocytes were induced by culturing CD34(+) cells from normal human peripheral blood in a serum-free liquid culture stimulated with thrombopoietin. The experiments contained the following groups: control cultures with nonirradiated cells incubated for 15 days; cultures treated with IL-3 on day 7 or day 11, cultures irradiated with 2 Gy on day 7 or day 11, and cultures treated with IL-3 immediately after X irradiation. The nonirradiated control cultures produced megakaryocytes from day 7, and both the megakaryocyte and platelet generation reached a peak on day 12-13. When X irradiation was performed on day 7, both the megakaryocyte and platelet numbers decreased remarkably, while no significant effect was observed on those numbers when cultures were X-irradiated on day 11. IL-3 showed neither protective nor promoting effects on the terminal stages of megakaryocytic maturation and platelet production. The results demonstrated that mature megakaryocytes are radiosensitive but that the radiosensitivity decreased with the terminal stages of megakaryocytic maturation, especially for the megakaryocytes entering into proplatelet formation.

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CD34+ progenitors are reproducibly recovered in thawed umbilical grafts, and positively influence haematopoietic reconstitution after transplantation

Cited by 24 publications

References 34 publications

Evaluation of DMSO dextrose as a suitable alternative for DMSO dextran in cord blood cryopreservation

Evaluation of DMSO dextrose as a suitable alternative for DMSO dextran in cord blood cryopreservation

The Cord Blood Apgar: a novel scoring system to optimize selection of banked cord blood grafts for transplantation (CME)

Radiation Sensitivities in the Terminal Stages of Megakaryocytic Maturation and Platelet Production

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