CD31hiEmcnhi Vessels Support New Trabecular Bone Formation at the Frontier Growth Area in the Bone Defect Repair Process

Wang, Jimeng; Gao, Yi; Cheng, Pengzhen; Li, Donglin; Jiang, Huijie; Ji, Chuanlei; Zhang, Shuaishuai; Shen, Chao; Li, Junqin; Song, Yong; Cao, Tianqing; Wang, Chunmei; Yang, Liu; Pei, Guoxian

doi:10.1038/s41598-017-04150-5

Cited by 31 publications

(26 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Maybe osteogenic cells from the periosteum and the dura show a differential responsiveness to TGF‐β and other bone‐derived molecules. BCM might as well control other mechanisms relevant for bone regeneration, for example, angiogenesis (Harnik, Miron, Buser, & Gruber, ; Wang et al., ). Overall, collagen membranes have an inherent biological activity by their modulation of the local concentration and activity of TGF‐β and likely other molecules modulating bone regeneration.…”

Section: Discussionmentioning

confidence: 99%

“…In support of this hypothesis are data that collagen adsorbes members of the TGF‐β‐superfamily including bone morphogenetic proteins (Freitas et al., ; Hempel et al., ) and that rat calvaria defects are rich in osteoblast progenitors (Wang & Glimcher, ). To which extent cells from the cambium layer of the periosteum (Gruber et al., ), from the blood capillaries (Wang et al., ), and from the dura mater (Petrie Aronin et al., ) contribute to bone formation in the two areas of interest, and if they are differentially targeted by BCM, remains to be determined. Moreover, we cannot rule out that the islands are connected to the host bone via unmineralized, osteoid‐like bridges that are not detected by the segmentation of μCT data.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Bone‐conditioned medium modulates the osteoconductive properties of collagen membranes in a rat calvaria defect model

Küchler

Rybaczek

Dobask

et al. 2018

Clinical Oral Implants Res

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Bone‐conditioned medium modulates the osteoconductive properties of collagen membranes in a rat calvaria defect model

Küchler

Rybaczek

Dobask

et al. 2018

Clinical Oral Implants Res

View full text Add to dashboard Cite

show abstract

“…Regarding the choice of animal model, nude mice are ideal for assessing ectopic bone formation, as these immunocompromised animals lack a thymus and do not reject the implant, so that the cells may easily survive after transplantation (34,35). However, rats are used for the classic animal model of bone defect (36,37). In the present study, the X-ray results revealed that the application of BMSCs+CPC and UCMSCs+CPC in the tibia defect model led to improved levels of osteogenesis compared with that in the CPC only group.…”

Section: Discussionmentioning

confidence: 99%

Comparative evaluation of the osteogenic capacity of human mesenchymal stem cells from bone marrow and umbilical cord tissue

Wang¹,

Zhao²,

Xing³

et al. 2018

Exp Ther Med

View full text Add to dashboard Cite

Mesenchymal stem cells (MSCs) have been extensively investigated in the field of regenerative medicine. Human bone MSCs (BMSCs) have become a common type of seed cell for bone tissue engineering. However, the viability and cell number of BMSCs are negatively correlated with donor age, and as the extraction process is painful, this method has not been widely used. As human umbilical cord MSCs (UCMSCs) may be harvested inexpensively and inexhaustibly, the present study evaluated and compared the regenerative potential of UCMSCs and BMSCs to determine whether UCMSCs may be used as a novel cell type for bone regeneration. In the present study, the proliferation and osteogenic capacity of BMSCs and UCMSCs was compared in vitro. BMSCs and UCMSCs were respectively combined with biofunctionalized macroporous calcium phosphate cement, and their bone regenerative potentials were determined by investigating their capacity for ectopic bone formation in a nude mouse model as well as their efficacy in a rat model of tibia bone defect. The extent of bone regeneration was examined by X-ray, histological and immunohistochemical analyses. The results revealed that UCMSCs exhibited a good osteogenic differentiation potential, similarly to that of BMSCs, and that UCMSCs were able to contribute to the regeneration of bone and blood vessels. Furthermore, no significant differences were identified between BMSCs and UCMSCs in terms of their bone regenerative effect.

show abstract

“…Bone defect repair involves a series of complex physiological and pathological processes 2) . Comminuted fractures, bone tumors, extractions and infections often result in local loss of bone tissue in varying degrees 3) .…”

Section: Introductionmentioning

confidence: 99%

Withdrawal: Synthesis of a Novel bFGF/nHAP/COL Bone Tissue Engineering Scaffold for Mandibular Defect Regeneration in a Rabbit Model

Yue

Tan

Zhao

et al. 2018

J. Hard Tissue Biology.

View full text Add to dashboard Cite

In bone tissue engineering, scaffold fabrication and biocompatibility are crucial concerns. Many scaffold materials have been explored, among which nanometer hydroxyapatite (nHAP) and collagen (COL) are commonly used. Additionally, growth factors can be used to modify scaff olds. In this study, lyophilization technology was used to build a scaff old comprising basic fi broblast growth factor (bFGF), nHAP and COL for the fi rst time. The resulting scaff old was characterized. bFGF release from the scaff old was assessed by ELISA. Bone marrow mesenchymal stem cells (BMSCs) were prepared and seeded onto the scaff old to test in vitro biological compatibility. A scanning electron microscope was used to observe the scaff old and evaluate BMSC morphology, and the cells were counted to detect early cell adhesion. Cell proliferation and activity were assessed by a cell counting kit-8 assay and measurement of alkaline phosphatase activity, respectively. Bilateral mandibular defects were prepared in 12 New Zealand rabbits and repaired using scaff olds. The rabbits were divided into four groups: a group treated with allogeneic BMSC-seeded bFGF/nHAP/COL scaff old, a group treated with allogeneic BMSC-seeded nHAP/COL scaff old, a group treated with nHAP/COL scaff old alone, and an untreated control group. After 12 weeks, three-dimensional computerized tomography examination, computerized tomography value measurement, gross observation and hematoxylin and eosin stain staining were conducted. SPSS17.0 software was used for data analysis. The gross morphology conformed to the characteristics of a tissue engineering scaff old. The bFGF/nHAP/COL scaff old promoted BMSC adhesion, proliferation and diff erentiation and hence promoted good bone formation, without exhibiting biological toxicity. Our fi ndings show that the bFGF/nHAP/COL scaff old has good physical properties and biocompatibility in vitro, and can be used to promote osteogenesis after in vivo implantation.

show abstract

CD31hiEmcnhi Vessels Support New Trabecular Bone Formation at the Frontier Growth Area in the Bone Defect Repair Process

Cited by 31 publications

References 38 publications

Bone‐conditioned medium modulates the osteoconductive properties of collagen membranes in a rat calvaria defect model

Bone‐conditioned medium modulates the osteoconductive properties of collagen membranes in a rat calvaria defect model

Comparative evaluation of the osteogenic capacity of human mesenchymal stem cells from bone marrow and umbilical cord tissue

Withdrawal: Synthesis of a Novel bFGF/nHAP/COL Bone Tissue Engineering Scaffold for Mandibular Defect Regeneration in a Rabbit Model

Contact Info

Product

Resources

About