CD and DSC Investigation of Individual and Complex Influence of Meso-Tetra(4-Oxiethylpyridil) Porphyrin (TOEPyP4) and Its Zn-Complex on DNA

Monaselidze, Jamlet; Kiziria, Evgeni; Gorgoshidze, M. Z.; Khachidze, D.; Kiladze, Maia; Lomidze, E. M.; Margaryan, H. L.; Hakobyan, N. H.

doi:10.4236/ajac.2012.310092

Cited by 12 publications

(2 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These binding modes are reflected on CD spectra as two positive bands with maxima at about 420 nm and 440 nm (Figure 4(c)). The intensities of these bands are significantly higher in comparison with the some bands at 20˚C at identical TOEPyP4/DNA ratios [12]. Consequently, the influence of Zn-TOEPyP4 complex on DNA at 37˚C is more effective than at 20˚C.…”

Section: Discussionmentioning

confidence: 77%

Zn Ions Change Binding Mode of TOEPyP4 with DNA and Cause DNA Transition from B to C and Zn-Like Conformations

Monaselidze¹,

Gorgoshidze²,

Khachidze³

et al. 2013

AJAC

Self Cite

View full text Add to dashboard Cite

It is known that at low concentrations of TMPyP4, this porphyrin predominantly intercalates between GC pairs at GC-rich sites of duplex DNA and G-quadruplexes of various constructions, and stabilizes these structures. However, there are still some arguable suggestions about the exact binding sites and modes of TMPyP4 to GC-rich regions of DNA in case of helation of divalent ions with help of the porphrin, which makes porphyrin structure asymmetric. We examined TOEPyP4-analogue of TMPyP4-and studied interaction of TOEPyP4 into the calf thymus DNA at presence of nanomole concentrations of one of the most important microelements in cell vital function-Zn ion. On the basis of CD and absorption spectra of the DNA-TOEPyP4 mixture, it was determined that nanomole concentrations of Zn ions changed porphyrin intercalative binding mode to some external binding modes, which initiated transition of the canonic B conformation of DNA into C-like conformation, and incubation of the (DNA-TOEP4) + Zn mixture at 37˚C caused B-Z-like transition, but no transition was observed for the DNA-TOEPyP4 mixture. In particular, at 10 mM·NaCl, TOEPyP4/DNA = 0.02, the binding mode change was observed in the concentration range from 150 to 300 nM·Zn, and the B-C-like transition occurred from 150 to 600 nM·Zn. The B-Z transition at TOEPyP4/DNA = 0.015, Zn/DNA = 0.015, NaCI 10 mM, T = 37˚C was observed within incubation time interval from 0.3 to 20 hours, and maximal percents of Z-like form was seen when incubation time interval was from 5 to 6 hours.

show abstract

Section: Discussionmentioning

confidence: 77%

Zn Ions Change Binding Mode of TOEPyP4 with DNA and Cause DNA Transition from B to C and Zn-Like Conformations

Monaselidze¹,

Gorgoshidze²,

Khachidze³

et al. 2013

AJAC

Self Cite

View full text Add to dashboard Cite

show abstract

“…The CD spectrum of CT‐DNA exhibited a canonical β‐conformation with a large positive band around 270–280 nm, a negative band ∼ 248 nm and a small positive band ∼ 210–220 nm (Figure ). Upon addition of Mn(II) complexes ( 1 ‐ 4 ), an enhancement of the molar ellipticity of the DNA band 270–280 nm was noted which suggest that the complexes bind DNA with the retention of the overall β‐conformation of free DNA …”

Section: Resultsmentioning

confidence: 99%

Amidooxime‐Based Mononuclear Mn(II) Complexes: Synthesis, Characterization, and Studies on DNA Binding and Nuclease Activity

et al. 2018

View full text Add to dashboard Cite

A number of new amidooxime based manganese(II) complexes have been synthesized and characterized by single crystal X‐ray diffraction studies as well as by geometry optimization of complex 2 using DFT/B3LYP. All four complexes (1‐4) are mononuclear having octahedral geometry. DNA binding interaction and nuclease activity of these compounds were investigated for diverse biomedical applications. The results of photophysical studies indicated that the Mn(II) compounds bind with DNA by intercalation mode and the complex 1 containing pyrimidine moiety and chloro ligation showed highest binding affinity. The binding affinities of these compounds were of the order of 104‐105 M−1 and all of them were found to be capable of inducing DNA cleavage.

show abstract

Applying Cu(II) complexes assisted by water‐soluble porphyrin to DNA binding and selective anticancer activities

Zhang

Liu

2020

Applied Organom Chemis

View full text Add to dashboard Cite

Cancer is one of the killers endangering human health and its treatment has always been a focus of the medical community. For anticancer drugs, water‐soluble porphyrin and Schiff bases have always been of interest. We report here three Cu(II)‐based complexes functionalized by water‐soluble cationic porphyrin and hydrazine Schiff base, which were prepared and evaluated for their biological activity. The three Cu(II) complexes all exhibited potent binding affinity to calf thymus DNA, the strongest interaction being between CuP2 and DNA. We studied the cytotoxicity of the complexes and ligands against different types of cancer cells (A549, H‐1975, HepG2 and T47D), results showing the ligands are less cytotoxic; therefore, the anticancer activity of the complexes is improved by complexation. Furthermore, the cytotoxicity of ligands and complexes was also evaluated against the normal cell line Hs 578Bst, complexes showing more negligible cytotoxicity than ligand. Moreover, the cellular uptake of these Cu(II) complexes was investigated using the extraction method and results suggested that CuP2 exhibits the best cellular uptake towards H‐1975 cells. Interestingly, fluorescence microscopy experiments and flow cytometric analysis (cell cycle) were used to further investigate the potent anticancer activities.

show abstract

CD and DSC Investigation of Individual and Complex Influence of Meso-Tetra(4-Oxiethylpyridil) Porphyrin (TOEPyP4) and Its Zn-Complex on DNA

Cited by 12 publications

References 23 publications

Zn Ions Change Binding Mode of TOEPyP4 with DNA and Cause DNA Transition from B to C and Zn-Like Conformations

Zn Ions Change Binding Mode of TOEPyP4 with DNA and Cause DNA Transition from B to C and Zn-Like Conformations

Amidooxime‐Based Mononuclear Mn(II) Complexes: Synthesis, Characterization, and Studies on DNA Binding and Nuclease Activity

Applying Cu(II) complexes assisted by water‐soluble porphyrin to DNA binding and selective anticancer activities

Contact Info

Product

Resources

About