CCCTC-binding factor controls the homeostatic maintenance and migration of Langerhans cells

Kim, Tae Kyun; Lee, Jong Joo; Kim, Sung Hee; Je, Jeong Hwan; Lee, Yangsin; Song, Min; Choi, Yun Young; Chung, Youn Wook; Park, Chae Gyu; Cho, Jin Won; Lee, Min Geol; Lee, Yeon Su; Kim, Hyoung‐Pyo

doi:10.1016/j.jaci.2015.03.033

Cited by 17 publications

(29 citation statements)

References 75 publications

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“…Isolation and flow cytometric characterization of DC subsets was performed according to our previous work and other studies as indicated in the Supplementary Materials (Guilliams et al, 2016;Kim et al, 2015;Tamoutounour et al, 2013).…”

Section: Cell Isolation and Flow Cytometrymentioning

confidence: 99%

“…Pre-cDCs from CD45.2 þ BM were determined and sorted as described in our previous study (Kim et al, 2015). Total 2 Â 10 6 CD45.1 þ supporting BM cells and 1 Â 10 4 sorted CD45.2 þ pre-cDCs were gently mixed and cultured in the complete RPMI medium containing FLT3L-conditioned supernatant with or without additional GM-CSF supplement.…”

Section: Pre-cdc Sorting and Mixed Culturementioning

confidence: 99%

“…Total RNA isolation, cDNA synthesis, and quantitative real-time PCR were performed as described in our previous study (Kim et al, 2015). Results were normalized to Hprt mRNA.…”

Section: Real-time Quantitative Polymerase Chain Reactionmentioning

confidence: 99%

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Skin-Specific CD301b+ Dermal Dendritic Cells Drive IL-17−Mediated Psoriasis-Like Immune Response in Mice

Kim

Park

et al. 2018

Journal of Investigative Dermatology

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Conventional dendritic cells (cDCs) are composed of heterogeneous subsets commonly arising from dendritic cell (DC)-committed progenitors. A population of CD301b-expressing DCs has recently been identified in non-lymphoid barrier tissues such as skin. However, whether CD301b DCs in the skin represent an ontogenetically unique subpopulation of migratory cDCs has not been fully addressed. Here, we demonstrated that CD301b dermal DCs were distinct subpopulation of FMS-like tyrosine kinase 3 ligand (FLT3L)-dependent CD11b cDC2 lineage, which required an additional GM-CSF cue for the adequate development. Although the majority of lymphoid-resident cDC2 lacked CD301b expression, dermal migratory cDC2 contained a substantial fraction of CD301b subset. Similar to CD301b population, CD301b dermal DC development was closely regulated by FLT3 signaling, suggesting their common origin from FLT3L-responsive cDC progenitors. However, FLT3L-driven cDC progenitor culture was not sufficient, but additional GM-CSF treatment was required to produce CD301b cDC2. In vivo development of CD301b cDC2 was significantly augmented by exogenous GM-CSF, while the repopulation of CD301b dermal cDC2 was abrogated by GM-CSF neutralization. Functionally, CD301b cDC2 was capable of producing a high level of IL-23, and the depletion of CD301b cDC2 effectively prevented IL-17-mediated psoriasiform dermatitis. Therefore, our findings highlight the differentiation program of a distinct CD301b dermal cDC2 subset in the skin and its involvement in psoriatic inflammation.

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Section: Cell Isolation and Flow Cytometrymentioning

confidence: 99%

Section: Pre-cdc Sorting and Mixed Culturementioning

confidence: 99%

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Skin-Specific CD301b+ Dermal Dendritic Cells Drive IL-17−Mediated Psoriasis-Like Immune Response in Mice

Kim

Park

et al. 2018

Journal of Investigative Dermatology

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“…Preparation of skin tissues was done according to our previous studies [31,32]. Briefly, excised skin was digested in 2.5 mg/ml dispase II (Roche, GmbH, Mannheim, Germany) to separate epidermal and dermal sheet.…”

Section: Tissue Preparation and Flow Cytometrymentioning

confidence: 99%

Resident and monocyte-derived Langerhans cells are required for imiquimod-induced psoriasis-like dermatitis model

Lee

Kim

et al. 2018

Journal of Dermatological Science

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“…20 Among them, CCCTC binding factor (CTCF) can promote the outflow of LCs from the epidermis. 34 CTCF plays a variety of roles in the haematopoietic lineage, which can regulate the early development of thymocytes and participate in the development and the operation of macrophages. 35,36 Gr-1 (also known as Ly-6c/G) is a monocyte with high expression of monocyte markers, and it is the precursor of LC in inflammatory epidermis.…”

Section: Mig R Ation Of L Ang Erhans Cell S During Homeos Ta S Is Amentioning

confidence: 99%

The role of Langerhans cells in epidermal homeostasis and pathogenesis of psoriasis

Yan

Liu

et al. 2020

J Cellular Molecular Medi

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Psoriasis is an inflammatory epidermal proliferative dermatosis which affects 2%-3% of the world's population. 1 Clinically, psoriasis is characterized by clear boundaries of skin lesions, erythema and scales on the skin. 2 Psoriasis was not initially considered as a disease, but a dysfunction of keratinocytes in the epidermis. 3 A large number of studies have shown that the innate and adaptive immune responses of cells, especially the T-cell-mediated system, play an important role in the pathogenesis of psoriasis. 4 In addition, the cytokine network is a key element in psoriasis. The expression levels of interleukin (IL)-1, tumour necrosis factor (TNF), interleukin-12 (IL-12), interleukin-17 (IL-17), interleukin-22 (IL-22) and interleukin-23 (IL-23) in psoriatic skin are significantly increased. Among them, IL-17A and IL-22 have the most profound effects on keratinocytes. 5 In psoriatic lesions, keratinocytes are activated and proliferate much faster than normal keratinocytes. 6 Not only do cytokines have a strong influence on psoriasis but many immune cells also change greatly in psoriasis. Inflammatory cells in psoriatic skin are mainly composed of dendritic cells (DCs), macrophages, dermal T cells and epidermal neutrophils. 7 Among these immune cells, dendritic cells increase significantly in psoriatic lesions. Although DCs play a central role in the pathogenesis of psoriasis, the specific role of each DC is not clear. At present, it is believed that psoriasis is caused by the imbalance

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CCCTC-binding factor controls the homeostatic maintenance and migration of Langerhans cells

Abstract: Our results show that CTCF positively regulates the homeostatic pool and the efficient emigration of LCs, which are required for modulating the functional immune network of the skin.

Cited by 17 publications

References 75 publications

Skin-Specific CD301b+ Dermal Dendritic Cells Drive IL-17−Mediated Psoriasis-Like Immune Response in Mice

Skin-Specific CD301b+ Dermal Dendritic Cells Drive IL-17−Mediated Psoriasis-Like Immune Response in Mice

Resident and monocyte-derived Langerhans cells are required for imiquimod-induced psoriasis-like dermatitis model

The role of Langerhans cells in epidermal homeostasis and pathogenesis of psoriasis

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