Cbf11 and Cbf12, the fission yeast CSL proteins, play opposing roles in cell adhesion and coordination of cell and nuclear division

Převorovský, Martin; Groušl, Tomáš; Staňurová, Jana; Rynes, Jan; Nellen, Wolfgang; Půta, František; Folk, Petr

doi:10.1016/j.yexcr.2008.12.001

Cited by 30 publications

(101 citation statements)

References 58 publications

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“…20,25,26 In the present study, we show that Cbf11 directly regulates the transcription of several genes required to prevent catastrophic mitosis (Fig. 5D).…”

Section: Discussionsupporting

confidence: 61%

“…30,31 Since the Cut6 enzyme requires biotin for its function, we selected these 2 genes for further analysis. The promoter of vht1 contains 2 Cbf11 response elements (GTGGGAA), 24,25 both corresponding to peaks of Cbf11 ChIP-seq signal; a variant GTGAGTA motif is located in the center of the Cbf11 ChIP-seq peak in the bio2 promoter (data not shown). As expected, maximum Cbf11 binding to vht1 and bio2 promoters was detected in S phase/cytokinesis by cell-cycle timecourse ChIP (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…S1, and refs. 25,26). The 'cut' phenotype was also described in cells bearing a temperature-sensitive mutation in cut6 (cut6-621), an essential gene encoding an acetyl-CoA/biotin carboxylase, when grown at 36…”

Section: Resultsmentioning

confidence: 99%

“…21 Fungal CSL proteins also exist, 22,23 with Cbf11 and Cbf12, the CSL proteins of fission yeast, also functioning as transcription factors. 24 Cbf11 and Cbf12 have been implicated in the control of cell-cycle progression, 25,26 and regulate directly and indirectly the expression of »80 cell-cycle periodic genes. 26 Cbf11 also regulates several lipid metabolism genes.…”

Section: Introductionmentioning

confidence: 99%

“…26 Interestingly, cells lacking cbf11 frequently undergo catastrophic mitosis and display the 'cut' phenotype. 25 Furthermore, the penetrance of the 'cut' phenotype in Dcbf11 cells is affected by growth media composition. 26 However, the molecular mechanism underlying this severe cellcycle defect is not known.…”

Section: Introductionmentioning

confidence: 99%

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CSL protein regulates transcription of genes required to prevent catastrophic mitosis in fission yeast

et al. 2016

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For every eukaryotic cell to grow and divide, intricately coordinated action of numerous proteins is required to ensure proper cell-cycle progression. The fission yeast Schizosaccharomyces pombe has been instrumental in elucidating the fundamental principles of cell-cycle control. Mutations in S. pombe 'cut' (cell untimely torn) genes cause failed coordination between cell and nuclear division, resulting in catastrophic mitosis. Deletion of cbf11, a fission yeast CSL transcription factor gene, triggers a 'cut' phenotype, but the precise role of Cbf11 in promoting mitotic fidelity is not known. We report that Cbf11 directly activates the transcription of the acetyl-coenzyme A carboxylase gene cut6, and the biotin uptake/ biosynthesis genes vht1 and bio2, with the former 2 implicated in mitotic fidelity. Cbf11 binds to a canonical, metazoan-like CSL response element (GTGGGAA) in the cut6 promoter. Expression of Cbf11 target genes shows apparent oscillations during the cell cycle using temperature-sensitive cdc25-22 and cdc10-M17 block-release experiments, but not with other synchronization methods. The penetrance of catastrophic mitosis in cbf11 and cut6 mutants is nutrient-dependent. We also show that drastic decrease in biotin availability arrests cell proliferation but does not cause mitotic defects. Taken together, our results raise the possibility that CSL proteins play conserved roles in regulating cell-cycle progression, and they could guide experiments into mitotic CSL functions in mammals.

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“…20,25,26 In the present study, we show that Cbf11 directly regulates the transcription of several genes required to prevent catastrophic mitosis (Fig. 5D).…”

Section: Discussionsupporting

confidence: 61%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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CSL protein regulates transcription of genes required to prevent catastrophic mitosis in fission yeast

et al. 2016

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Current awareness on yeast

2009

Yeast

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In order to keep subscribers up‐to‐date with the latest developments in their field, this current awareness service is provided by John Wiley & Sons and contains newly‐published material on yeasts. Each bibliography is divided into 10 sections. 1 Reviews; 2 General; 3 Biochemistry; 4 Biotechnology; 5 Cell Biology; 6 Gene Expression; 7 Genetics; 8 Physiology; 9 Medical Mycology; 10 Recombinant DNA Technology. Within each section, articles are listed in alphabetical order with respect to author. If, in the preceding period, no publications are located relevant to any one of these headings, that section will be omitted. (5 weeks journals ‐ search completed 5th. Aug. 2009)

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pREPORT: a multi‐readout transcription reporter vector for fission yeast

Převorovský

2014

Yeast

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Transcription factors are prominent regulators of gene expression that execute responses to various intracellular and extracellular stimuli. Recombinant transcription reporter systems can be conveniently used to study the DNA binding preferences and regulatory activity of a transcription factor under a range of conditions. Several reporter genes have been used to study transcription regulation in the fission yeast Schizosaccharomyces pombe. Each of these reporters has distinct advantages, such as high sensitivity or ease of use, and limitations, such as prohibitive costs or use of hazardous substances. To combine the strengths and mitigate the weaknesses of individual reporter genes, we have created pREPORT, a flexible multi-readout transcription reporter vector for fission yeast that employs an enhanced GFP-lacZ fusion and a customizable minimal promoter. With pREPORT, gene expression driven by the transcription factor of interest can be quantified in a number of ways, both in live cells and in vitro, using a single reporter construct.

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Cbf11 and Cbf12, the fission yeast CSL proteins, play opposing roles in cell adhesion and coordination of cell and nuclear division

Cited by 30 publications

References 58 publications

CSL protein regulates transcription of genes required to prevent catastrophic mitosis in fission yeast

CSL protein regulates transcription of genes required to prevent catastrophic mitosis in fission yeast

Current awareness on yeast

pREPORT: a multi‐readout transcription reporter vector for fission yeast

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