Cationic solid lipid nanoparticles derived from apolipoprotein-free LDLs for target specific systemic treatment of liver fibrosis

Kong, Won Ho; Park, Kitae; Lee, Min Young; Lee, Hwiwon; Sung, Dong Kyung; Hahn, Sei Kwang

doi:10.1016/j.biomaterials.2012.09.067

Cited by 63 publications

(35 citation statements)

References 37 publications

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“…While eosin is an acidic dye which stains cytoplasm red or pink. 30,31 With the increasing H 2 O 2 concentration, the degree of injury of cells increases, which is mainly manifested in the reduced cell number and disordered cell morphology ( Figure 4). The number of cells in the nano-COM-and COD-treated group was less than the cells in the control group, which was only treated with H 2 O 2 .…”

Section: Cell Morphological Observation By Hande Stainingmentioning

confidence: 99%

Reinjury risk of nano-calcium oxalate monohydrate and calcium oxalate dihydrate crystals on injured renal epithelial cells: aggravation of crystal adhesion and aggregation

Gan¹,

Sun²,

Bhadja³

et al. 2016

IJN

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Background Renal epithelial cell injury facilitates crystal adhesion to cell surface and serves as a key step in renal stone formation. However, the effects of cell injury on the adhesion of nano-calcium oxalate crystals and the nano-crystal-induced reinjury risk of injured cells remain unclear. Methods African green monkey renal epithelial (Vero) cells were injured with H 2 O 2 to establish a cell injury model. Cell viability, superoxide dismutase (SOD) activity, malonaldehyde (MDA) content, propidium iodide staining, hematoxylin–eosin staining, reactive oxygen species production, and mitochondrial membrane potential (Δψm) were determined to examine cell injury during adhesion. Changes in the surface structure of H 2 O 2 -injured cells were assessed through atomic force microscopy. The altered expression of hyaluronan during adhesion was examined through laser scanning confocal microscopy. The adhesion of nano-calcium oxalate monohydrate (COM) and calcium oxalate dihydrate (COD) crystals to Vero cells was observed through scanning electron microscopy. Nano-COM and COD binding was quantitatively determined through inductively coupled plasma emission spectrometry. Results The expression of hyaluronan on the cell surface was increased during wound healing because of Vero cell injury. The structure and function of the cell membrane were also altered by cell injury; thus, nano-crystal adhesion occurred. The ability of nano-COM to adhere to the injured Vero cells was higher than that of nano-COD crystals. The cell viability, SOD activity, and Δψm decreased when nano-crystals attached to the cell surface. By contrast, the MDA content, reactive oxygen species production, and cell death rate increased. Conclusion Cell injury contributes to crystal adhesion to Vero cell surface. The attached nano-COM and COD crystals can aggravate Vero cell injury. As a consequence, crystal adhesion and aggregation are enhanced. These findings provide further insights into kidney stone formation.

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Section: Cell Morphological Observation By Hande Stainingmentioning

confidence: 99%

Reinjury risk of nano-calcium oxalate monohydrate and calcium oxalate dihydrate crystals on injured renal epithelial cells: aggravation of crystal adhesion and aggregation

Gan¹,

Sun²,

Bhadja³

et al. 2016

IJN

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“…55 Among the several pharmaceutical methods that have been used to increase the oral absorption of puerarin or decrease the risk of serious adverse effects of its injection, lipid nanoparticles have gained attention because of their biocompatibility and organ-targeting capability, especially their potential for targeted brain drug delivery. [56][57][58][59][60] Therefore, puerarinloaded SLN and NLC particles with blends of glyceryl monostearate (solid lipid phase) and capric/caprylic triglyceride (liquid lipid phase) as the lipid matrices were used. Interestingly, contrary to many other reports, this study reports that SLN exhibited a smaller size than NLC.…”

Section: Impact Of Variables On Size Of Slns and Nlcsmentioning

confidence: 99%

The Impact of Variables on Particle Size of Solid Lipid Nanoparticles and Nanostructured Lipid Carriers; A Comparative Literature Review

Bahari¹,

Hamishehkar²

2016

Adv Pharm Bull

172

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“…To date there is no effective therapy for the treatment of hepatic fibrosis [35]. In the long run liver fibrosis changes into cirrhosis characterized by appearance of regenerative nodules, increasing the risk of portal hypertension and hepatocellular carcinoma [36].…”

Section: Ras and Liver Fibrosismentioning

confidence: 99%

Role of renin-angiotensin system in liver diseases: an outline on the potential therapeutic points of intervention

Ahmadian

Pennefather

Eftekhari

et al. 2016

Expert Review of Gastroenterology & Hepatology

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The current review aimed to outline the functions of the renin angiotensin system (RAS) in the context of the oxidative stress-associated liver disease. Areas covered: Angiotensin II (Ang II) as the major effector peptide of the RAS is a pro-oxidant and fibrogenic cytokine. Mechanistically, NADPH oxidase (NOX) is a multicomponent enzyme complex that is able to generate reactive oxygen species (ROS) as a downstream signaling pathway of Ang II which is expressed in liver. Ang II has a detrimental role in the pathogenesis of chronic liver disease through possessing pro-oxidant, fibrogenic, and pro-inflammatory impact in the liver. The alternative axis (ACE2/Ang(1-7)/mas) of the RAS serves as an anti-inflammatory, antioxidant and anti-fibrotic component of the RAS. Expert commentary: In summary, the use of alternative axis inhibitors accompanying with ACE2/ Ang(1-7)/mas axis activation is a promising new strategy serving as a novel therapeutic option to prevent and treat chronic liver diseases.

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Cationic solid lipid nanoparticles derived from apolipoprotein-free LDLs for target specific systemic treatment of liver fibrosis

Cited by 63 publications

References 37 publications

Reinjury risk of nano-calcium oxalate monohydrate and calcium oxalate dihydrate crystals on injured renal epithelial cells: aggravation of crystal adhesion and aggregation

Reinjury risk of nano-calcium oxalate monohydrate and calcium oxalate dihydrate crystals on injured renal epithelial cells: aggravation of crystal adhesion and aggregation

The Impact of Variables on Particle Size of Solid Lipid Nanoparticles and Nanostructured Lipid Carriers; A Comparative Literature Review

Role of renin-angiotensin system in liver diseases: an outline on the potential therapeutic points of intervention

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