Cationic Polystyrene Nanoparticles: Preparation and Characterization of a Model Drug Carrier System for Antisense Oligonucleotides

Spontaneous Formation of Nucleic Acid-based Nanoparticles Is Responsible for High Interferon-α Induction by CpG-A in Plasmacytoid Dendritic Cells

“…Nanoparticle Preparation and Formation of Nanoparticle-ODN Conjugates-Polystyrene nanoparticles were prepared by batch polymerization under surfactant-free conditions as described by Fritz et al (26). The cationic initiator AIBI was applied to induce positive surface charge.…”

Quantitative Analysis of Modified Antisense Oligonucleotides in Biological Fluids Using Cationic Nanoparticles for Solid-Phase Extraction

“…In contrast to other techniques using strong anion exchange resins (15)(16)(17), this method is based on the application of cationic polystyrene nanoparticles which are capable of reversibly binding negatively charged oligonucleotides with high affinity (18). Strong attractive forces between the ONs and the particle surface are mediated by hydrophobic as well as multiple electrostatic interactions.…”

Separation of cationic polymer particles and characterization of avidin‐immobilized particles by capillary electrophoresis

“…For example, the size of metal nanoparticles is an important factor in determining their physical and chemical properties. Size-exclusion chromatography (SEC) [1] is often used to separate colloidal particles with different size. In the case of particles adsorbed irreversibly on the packed SEC column, however, precise size measurements and separations would be difficult.…”

“…Although the difficulty in the treatment, cationic polymer particles have been investigated since the materials are very useful for modifier of paper [10], carrier of proteins [11], drug delivery [1], and gene carriers [12]. The use of cationic polymer particles as the carrier of proteins has several advantages; strong and stable immobilization via covalent bond between proteins and cationic polymer particles reduced desorption of proteins in comparison with the physical adsorption, so that particles immobilized proteins have been applied for molecular biology, e.g., affinity purification.…”