Catechol determination in compost bioremediation using a laccase sensor and artificial neural networks

Abstract

An electrochemical biosensor based on the immobilization of laccase on magnetic core-shell (Fe(3)O(4)-SiO(2)) nanoparticles was combined with artificial neural networks (ANNs) for the determination of catechol concentration in compost bioremediation of municipal solid waste. The immobilization matrix provided a good microenvironment for retaining laccase bioactivity, and the combination with ANNs offered a good chemometric tool for data analysis in respect to the dynamic, nonlinear, and uncertain characteristics of the complex composting system. Catechol concentrations in compost samples were determined by using both the laccase sensor and HPLC for calibration. The detection range varied from 7.5 × 10(-7) to 4.4 × 10(-4) M, and the amperometric response current reached 95% of the steady-state current within about 70 s. The performance of the ANN model was compared with the linear regression model in respect to simulation accuracy, adaptability to uncertainty, etc. All the results showed that the combination of amperometric enzyme sensor and artificial neural networks was a rapid, sensitive, and robust method in the quantitative study of the composting system.

Microbial Tyrosinases: Promising Enzymes for Pharmaceutical, Food Bioprocessing, and Environmental Industry

“…These nanoparticles used in electrochemical biosensors have the ability to provide a favorable microenvironment for biomolecules such as proteins to exchange electrons directly with an electrode [129]. Similarly, different enzyme based biosensors involving laccase [130, 131], tyrosinase [132], glucose oxidase [133, 134], horseradish peroxidase [135], and Fe 3 O 4 nanoparticles have been used for the construction of electrochemical biosensors. Tyrosinase electrode is also employed to monitor phenolic and catecholic compounds; it is one of the effective transducers for detection of phenols or catechols [85, 136, 137].…”

Interaction between Filler and Polymeric Matrix in Nanocomposites: Magnetic Approach and Applications

“…In the case of bioremediation, the use of an electrochemical biosensor based on the immobilization of laccase on magnetic core-shell (Fe 3 O 4 -SiO 2 ) NPs were combined with artificial neural networks for the determination of catechol concentration in it. The study results showed that combining an amperometric enzyme sensor and an artificial neural networks was effective because it presents sensitivity and is a robust method in the quantitative study of the composting system [158]. These and many more applications in bioremediation can be achieved with MNPs, and there is a whole world of possibilities awaiting.…”

Electrochemical DNA sensor for simultaneous detection of genes encoding two functional enzymes involved in lignin degradation

“…In the reaction, hydroquinone (QH 2 ) as electron donor for the oxidized form of the enzyme, is mainly converted into quinone and/or free radical product (Q), and then is reduced on the surface of gold electrode at potentials below 0 V (vs. SCE), which efficiently shuttle electrons between laccase redox center and gold electrode surface in a dynamical equilibrium, leading to detectable response current [41]. The reaction equations of the redox process on the electrode surface are described as follows:…”