Catch bonds

Hertig, Samuel; Vogel, Viola

doi:10.1016/j.cub.2012.08.035

Cited by 18 publications

(19 citation statements)

References 10 publications

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“…3e-i). Unlike previously described catch bond or cooperative binding cofactors 33,58 , such as allosteric regulation by neighbouring protein domains (that is, within the molecule) or soluble co-factors, our finding is distinct: the co-factor is a nearby co-receptor but not covalently associated with the first. Importantly, as circulating tumour cells are exposed to a complex haemodynamic environment during the their transit through the vasculature 59 , forces applied to non-covalent interactions between adhesion receptors are important perturbations that may regulate bond affinities and subsequent signalling.…”

Section: Discussioncontrasting

confidence: 47%

“…Singlemolecule approaches (that is, the biomembrane force probe (BFP)) have been extensively used to study force regulation of protein-protein interactions, determine protein conformational changes and understand signal initiation [28][29][30][31] , dissecting the relative contribution of individual proteins from complex molecular cohorts 32 . For example, single-molecule studies have elucidated the catch bond behaviour-bonds can be stabilized rather than disrupted by force 33,34 . Catch bonds are probably essential to key cell physiological events that occur in conditions where forces are externally applied or internally generated by the cell, such as adhesion enhancement of circulating cells and bacteria under shear stress or during receptor mechanosignalling [35][36][37] .…”

mentioning

confidence: 99%

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Dynamic catch of a Thy-1–α5β1+syndecan-4 trimolecular complex

Fiore

Chen

et al. 2014

Nat Commun

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Cancer cell adhesion to the vascular endothelium is a critical step of tumour metastasis. Endothelial surface molecule Thy-1 (CD90) is implicated in the metastatic process through its interactions with integrins and syndecans. However, how Thy-1 supports cell-cell adhesion in a dynamic mechanical environment is not known. Here we show that Thy-1 supports b 1 integrin-and syndecan-4 (Syn4)-mediated contractility-dependent mechanosignalling of melanoma cells. At the single-molecule level, Thy-1 is capable of independently binding a 5 b 1 integrin and syndecan-4 (Syn4) receptors. However, in the presence of both a 5 b 1 and Syn4, the two receptors bind cooperatively to Thy-1, to form a trimolecular complex. This trimolecular complex displays a unique phenomenon we coin 'dynamic catch', characterized by abrupt bond stiffening followed by the formation of catch bonds, where force prolongs the bond lifetime. Thus, we reveal a new class of trimolecular interactions where force strengthens the synergistic binding of two co-receptors and modulates downstream mechanosignalling.

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Section: Discussioncontrasting

confidence: 47%

mentioning

confidence: 99%

Dynamic catch of a Thy-1–α5β1+syndecan-4 trimolecular complex

Fiore

Chen

et al. 2014

Nat Commun

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“…This specificity resulted in the development of mannose drug analogs to inhibit bacterial attachment (23). In addition to being involved in attachment to abiotic surfaces, FimH primarily mediates strong adherence to mannose receptors using catch bonds, a type of bond that becomes stronger over time or becomes activated when the receptor and ligand are pulled apart (24, 25). Catch bonds are particularly effective for organisms that experience shear stress, such as those that colonize host mucosal surfaces or air-water interfaces.…”

Section: Introductionmentioning

confidence: 99%

Adhesins Involved in Attachment to Abiotic Surfaces by Gram-Negative Bacteria

et al. 2015

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During the first step of biofilm formation, initial attachment is dictated by physicochemical and electrostatic interactions between the surface and the bacterial envelope. Depending upon the nature of these interactions, attachment can be transient or permanent. To achieve irreversible attachment, bacterial cells have developed a series of surface adhesins promoting specific or non-specific adhesion under various environmental conditions. This chapter will review the recent advances in our understanding of the secretion, assembly and regulation of the bacterial adhesins during biofilm formation with a particular emphasis on the fimbrial, non-fimbrial and discrete polysaccharide adhesins in Gram-negative bacteria.

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“…]a This chip‐based system will allow us here 1) to prepare continuous gradients, 2) to modify them with carbohydrate binding ligands and 3) to probe the effect of shear stress on binding of E. coli by varying the flow speed. Apart from bacterial binding that is dependent on the surface density of mannose, the interaction strength has been shown to increase at higher shear stresses since binding occurs through unique catch bonds . As a result, the bacterium is able to mitigate the stresses from bodily fluid flow and cause infection .…”

Section: Introductionmentioning

confidence: 99%

A Microfluidic Device with Continuous Ligand Gradients in Supported Lipid Bilayers to Probe Effects of Ligand Surface Density and Solution Shear Stress on Pathogen Adhesion

Weerd

Sankaran

Roling

et al. 2016

Adv Materials Inter

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Studying binding interactions involving living cells requires a platform that carefully mimics the physiological parameters that govern these phenomena. Very often the amount of ligands that receptors can bind affect overall binding strength as is the case in cell adhesion. In addition, the physical environment can strongly influence these processes. This is exemplified by the effect of shear stress in catch‐bond‐mediated binding of bacteria. Traditional analysis techniques do not allow to probe these factors simultaneously. To this end, continuous ligand gradients in locked‐in supported lipid bilayers (SLBs) are prepared in a microfluidic device to control fluid flow. This platform allows for one‐pot characterization of cell surface binding events and 1) the effect of ligand density and 2) shear stress, simultaneously. The model interaction between the FimH receptor found on Escherichia coli and mannose found on the mammalian cell membrane is used to evaluate the platform. Using a single chip, specific E. coli ORN 178 adhesion (K d of 0.9 × 10−21 m), detachment and displacement are shown to depend on the mannose‐density and shear stress. For the first time, these effects are studied in a single chip device with high quality. This chip provides entry to further our understanding of other cell–cell interactions.

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Catch bonds

Cited by 18 publications

References 10 publications

Dynamic catch of a Thy-1–α5β1+syndecan-4 trimolecular complex

Dynamic catch of a Thy-1–α5β1+syndecan-4 trimolecular complex

Adhesins Involved in Attachment to Abiotic Surfaces by Gram-Negative Bacteria

A Microfluidic Device with Continuous Ligand Gradients in Supported Lipid Bilayers to Probe Effects of Ligand Surface Density and Solution Shear Stress on Pathogen Adhesion

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