volume 277, issue 25, P22648-22655 2002
DOI: 10.1074/jbc.m110420200
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Takao Koeduka, Kenji Matsui, Yoshihiko Akakabe, Tadahiko Kajiwara

Abstract: Long-chain fatty acids can be metabolized to C n؊1 aldehydes by ␣-oxidation in plants. The reaction mechanism of the enzyme has not been elucidated. In this study, a complete nucleotide sequence of fatty acid ␣-oxygenase gene in rice plants (Oryza sativa) was isolated. The deduced amino acid sequence showed some similarity with those of mammalian prostaglandin H synthases (PGHSs). The gene was expressed in Escherichia coli and purified to apparently homogenous state. It showed the highest activity with linole…

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