volume 16, issue 4, P266-275 1994
DOI: 10.1016/0141-0229(94)90165-1
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Keungarp Ryu, Jungbae Kim, Jonathan S. Dordick

Abstract: An extracellular protease has been isolated and partially purified from the extreme halophile Halobacterium halobium (ATCC 43214). The major enzyme component has a M(r) of 66,000 and is highly dependent upon salt concentrations near saturation for catalytic activity and stability. In aqueous solutions, a decrease in the NaCl concentration from 4 to 1 M results in an increase of nearly three orders of magnitude in the first-order rate constant of inactivation at 30 degrees C. Salt effects the stability of the e…

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