Catalpol ameliorates cognition deficits and attenuates oxidative damage in the brain of senescent mice induced by d-galactose

Zhang, Xiu Li; Li, Zhi Bo; Hao, Shuang; Liu, An

doi:10.1016/j.pbb.2007.07.004

Cited by 122 publications

(69 citation statements)

References 36 publications

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“…The assay for total SOD was based on its ability to inhibit the oxidation of hydroxylamine by the xanthinexanthine oxidase system [34,35]. SOD activity was measured at 550 nm by testing the inhibition degree of nitrite formation according to SOD detecting kit.…”

Section: Biochemical Analysismentioning

confidence: 99%

“…One unit of SOD is defined as the enzyme required to produce 50% inhibition of pyrogallol auto-oxidation, and date were expressed as units per milliliter of serum protein. MDA is produced during lipid peroxidation and can be determined by the thiobarbituric acid (TBA) test [23,34]. MDA reacted with TBA to form MDA-(TBA) 2 , a red-colored product with maximum absorbance at 532 nm.…”

Section: Biochemical Analysismentioning

confidence: 99%

“…The activity of SOD in brain tissues of C57BL/6J mice was detected by a total SOD assay kit according to the manufacturer's protocol [34,35]. Briefly, brain extracts, nitroblue tetrazolium, and enzyme-working solutions were prepared and added into a 96-well plate.…”

Section: Measurement Of Sod and Mda In Brain Tissuesmentioning

confidence: 99%

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Effects of Walnut Polyphenol on Learning and Memory Functions in Hypercholesterolemia Mice

Shi¹,

Chen²,

Zhao³

et al. 2014

JFNR

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Evidence suggests that dietary walnuts are able to induce improvements in memory and learning functions. In addition, polyphenols have been shown to modulate critical neuronal signalling pathways involved in processes of learning and memory. The aim of our present work was to study the effect of polyphenol extracts from walnut testa (42%) on learning and memory functions in hypercholesterolemia mice based on obesity, hypercholesterolemia and oxidative stress. At the beginning of the experiment, mice were divided into 3 groups, one of them served as normol control group (NCG), the second as hypercholesterolemia control group (HCG), the last as walnut polyphenol-treated group (WTG). After 8 weeks of treatment, we investigated the performance of C57BL/6J mice in Morris water maze test. The results showed that the escape latency was significant increase in HCG, when compared to NCG and WTG. In addition, the number of crossings was significant decrease in HCG, when compared to NCG and WTG (-47.35% and -43.35%, respectively, P < 0.01). The swimming distance was longer in HCG than NCG and WTG [F (2,96) = 44.45, P < 0.001]. However, there was no significant difference in swimming speed among NCG, HCG and WTG [F (2, 96) = 0.167, P > 0.05]. On the other hand, walnut polyphenol (WP) significantly decreased serum total triglycerides, cholesterol and malondialdehyde (MDA) level (-36.31%, -31.48% and -21.51%, respectively, P < 0.01) and increased superoxide dismutase (SOD) activity (+48.39%, P < 0.01). Administration of WP significantly decreased MDA level (-36.86%, P < 0.01) and increased SOD activity (+17.08%, P < 0.01) in brain tissues. In conclusion, walnut polyphenol was able to improve learning and memory functions.

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Section: Biochemical Analysismentioning

confidence: 99%

Section: Biochemical Analysismentioning

confidence: 99%

See 1 more Smart Citation

Effects of Walnut Polyphenol on Learning and Memory Functions in Hypercholesterolemia Mice

Shi¹,

Chen²,

Zhao³

et al. 2014

JFNR

View full text Add to dashboard Cite

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“…7,8) It has been shown that D-galtreated animal models show aging-related changes including the impairment of spatial learning and memory, object novelty preference, and locomotor activity, 9,10) and increased production of ROS and lowered activities of antioxidant enzymes. 11,12) Other studies demonstrated that long-term subcutaneous injection of D-gal in mice induced decreased immune responses and increased cell karyopyknosis, apoptosis, and caspase-3 protein levels in hippocampal neurons.11,13) Therefore mice continuously injected with D-gal have been extensively used for pharmacologic research on brain aging.(Ϫ)-Epigallocatechin-3-gallate (EGCG) is a member of the catechin family and a major polyphenolic constituent of green tea.14) It was reported that EGCG has potent ironchelating, antioxidant, antiinflammatory, anticancer, and neuroprotective activities. [15][16][17][18][19] In particular, EGCG has been shown to have neuroprotective effects by elevating the a-secretase activity of amyloid precursor protein (APP) and conversion to soluble APP-alpha (sAPP-a) and reducing amyloid beta (Ab)-induced neurotoxicity in a 1-methyl 4-phenyl 1,2,3,6-tetrahydropyridine (MPTP) mouse model, the "Swedish" APP transgenic (Tg2576) mouse model of Alzheimer's disease, and N2a cells stably transfected with "Swedish" mutant human APP.…”

mentioning

confidence: 99%

mentioning

confidence: 99%

Neuroprotective Effects of (-)-Epigallocatechin-3-gallate on Aging Mice Induced by D-Galactose

Zhao

Wei

et al. 2009

Biological & Pharmaceutical Bulletin

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Aging is a complicated multifactorial process in which a progressive decline in the physiologic function of organisms occurs. Oxidative stress and reactive oxygen species (ROS) have been proposed to be major cause of aging and other aging-related neurodegenerative conditions such as Alzheimer's disease.1,2) Aging-related oxidative damage in the brain and memory impairment mainly result from an imbalance between ROS generation and antioxidant enzyme activities. The overproduction of ROS or decrease in antioxidants in the brain can cause lipid peroxidation, nuclear and mitochondrial DNA damage, and protein oxidation and finally affect the normal functions of organisms. [3][4][5][6] D-Galactose (D-gal) is a reducing sugar normally present in the body. When present at levels greater than its normal content, it can be oxidized into aldehydes and hydrogen peroxide (H 2 O 2 ) by galactose oxidase. 7,8) It has been shown that D-galtreated animal models show aging-related changes including the impairment of spatial learning and memory, object novelty preference, and locomotor activity, 9,10) and increased production of ROS and lowered activities of antioxidant enzymes. 11,12) Other studies demonstrated that long-term subcutaneous injection of D-gal in mice induced decreased immune responses and increased cell karyopyknosis, apoptosis, and caspase-3 protein levels in hippocampal neurons.11,13) Therefore mice continuously injected with D-gal have been extensively used for pharmacologic research on brain aging.(Ϫ)-Epigallocatechin-3-gallate (EGCG) is a member of the catechin family and a major polyphenolic constituent of green tea.14) It was reported that EGCG has potent ironchelating, antioxidant, antiinflammatory, anticancer, and neuroprotective activities. [15][16][17][18][19] In particular, EGCG has been shown to have neuroprotective effects by elevating the a-secretase activity of amyloid precursor protein (APP) and conversion to soluble APP-alpha (sAPP-a) and reducing amyloid beta (Ab)-induced neurotoxicity in a 1-methyl 4-phenyl 1,2,3,6-tetrahydropyridine (MPTP) mouse model, the "Swedish" APP transgenic (Tg2576) mouse model of Alzheimer's disease, and N2a cells stably transfected with "Swedish" mutant human APP. 20,21) Furthermore, some studies have recently demonstrated that the neuroprotective mechanisms of EGCG are partly due to increasing activities of antioxidant enzymes and decreasing advanced glycation endproduct (AGE)-induced damage in aged rat brain or neuronal cells. 22,23) However, no study has been performed to evaluate whether EGCG has protective effects on aging mice induced by D-gal and its mechanisms of action. In the present study, we induced the aging mice model with D-gal (150 mg/kg/d, 6 weeks subcutaneously (s.c.)) and observed whether EGCG (2 mg/kg/d or 6 mg/kg/d, 4 weeks intragastrically (i.g.)) had potent antioxidant and antiapoptotic neuroprotective effects using behavioral testing and measurements of the activities of total superoxide dismutase (T-SOD) and glutathione peroxidase (GSH-Px), con...

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Effects of catalpol on bronchial asthma and its relationship with cytokines

Liu

Wang

Yang

2018

J of Cellular Biochemistry

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An animal (BALB/c mice) model of catalpol associated with bronchial asthma in vivo was established, and the effects of catalpol and its relationship with cytokines were investigated. A total of 30 adult BALB/c mice were randomly divided into a positive control group, a model group, and a catalpol group, with 10 mice in each group. The lung function of mice, the cell count, and the cytokine concentrations in bronchoalveolar lavage fluid (BALF) were detected.The levels of cytokines [interleukin 4 (IL-4), interleukin 5 (IL5), and interferon gamma (IFN-γ)] in BALF were measured with enzyme-linked immunosorbent assay methods. The total number of cells in the BALF of the group treated with catalpol was significantly lower than the model group. After treatment with catalpol, the eosinophils and neutrophils of the mice were remarkably reduced compared with the model group. The malondialdehyde content in the lung tissue homogenate of the mice was also decreased in the catalpol group. The cytokines IL-5 and IL-4 exhibited a similar tendency: the concentrations of IL-4 and IL-5 for the catalpol group were dramatically decreased compared with the model group. However, the IFN-γ concentration for the catalpol group was higher than the model group. The results indicated that IL-5 may involve in the pathologic process of asthma-like IL-4, and an inflammatory reaction may still exist in the airway during the remission stage of asthma. The imbalances of the cytokine network might be an important molecular basis in the asthma pathogenesis. It is suggested that catalpol may be a potential drug for the clinical treatment of asthma. K E Y W O R D S bronchial asthma, catalpol, IFN-γ, IL-4, IL-5 J Cell Biochem. 2019;120:8992-8998. wileyonlinelibrary.com/journal/jcb 8992 |

show abstract

Catalpol ameliorates cognition deficits and attenuates oxidative damage in the brain of senescent mice induced by d-galactose

Cited by 122 publications

References 36 publications

Effects of Walnut Polyphenol on Learning and Memory Functions in Hypercholesterolemia Mice

Effects of Walnut Polyphenol on Learning and Memory Functions in Hypercholesterolemia Mice

Neuroprotective Effects of (-)-Epigallocatechin-3-gallate on Aging Mice Induced by D-Galactose

Effects of catalpol on bronchial asthma and its relationship with cytokines

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