Catalase, superoxide dismutase, and glutathione peroxidase activities in various rat tissues after carbon tetrachloride intoxication

Szymonik-Lesiuk, S.; Czechowska, Grażyna; Stryjecka-Zimmer, Marta; Słomka, Maria; Mądro, Agnieszka; Celiński, Krzysztof; Wielosz, Marian

doi:10.1007/s00534-002-0824-5

Cited by 216 publications

(115 citation statements)

References 31 publications

(73 reference statements)

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“…This brief period might not be sufficient to demonstrate any rise in serum protein levels. Szymonik-Lesiuk et al (2003) have shown that CCl 4 intoxication can lead to alteration in kidney; these findings are in accordance with the present observation. In the present study, an increased frequency of glomerulosclerosis and tubulointerstitial alterations was found in rats with reduced renal mass on CCl 4 administration (Figure 4c), thereby indicating nephrotoxicity on CCl 4 administration in rats.…”

Section: Discussionsupporting

confidence: 82%

Hepatoprotective effect of Barrisal (herbal drug) on carbon tetrachloride induced hepatic damage in rats

Ahmed¹

2013

Afr. J. Pharm. Pharmacol.

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Barrisal is an herbal preparation of Hamdard Laboratories (Waqf) Pakistan that has been used as hepatoprotective agent. In this study, the protective effects of Barrisal against liver damage were evaluated in carbon tetrachloride (CCl 4 )-induced chronic hepatotoxicity in rats. Four groups (I, II, III, IV) of Sprague-Dawley (SD) rats were used and Groups I and II were orally fed with saline and Barrisal (10 ml/kg), respectively for 14 consecutive days while Groups III and IV were orally fed with saline, Barrisal (10 ml/kg) and then a single dose of CCl 4 (0.3 ml/kg body weight in a 20% olive oil) was injected intraperitoneally 30 min after the last dose of Barrisal and the animals were starved for 24 h. The degree of protection was measured using biochemical parameters like serum glutamate oxaloacetate transaminase (SGOT) and serum glutamate pyruvate transaminase (SGPT), alkaline phosphatase (ALP), total protein and albumin. Data obtained from results were compared using student's t-test (paired and unpaired) and one-way analysis of variance (ANOVA), significant differences were determined (Tukey honestly significant difference (HSD) test) using the Minitab 13 computing program. Values of P<0.05, were considered to be significant. The results showed that the treatment with Barrisal significantly lowered the CCl 4 -induced serum levels of hepatic enzyme markers (GOT, GPT, ALP, and total bilirubin, total cholesterol, total protein) indicated hepatoprotective effect of the Barrisal. Histopathological examination of liver sections confirmed that, pre-treatment with Barrisal reduced the hepatic damage induced by CCl 4 . Barrisal reduced the incidence of liver lesions including hepatic cells cloudy swelling, lymphocytes infiltration, hepatic necrosis, and fibrous connective tissue proliferation induced by CCl 4 in rats. Therefore, the results of this study suggest that Barrisal could protect liver against CCl 4 -induced oxidative damage in rats, and this hepatoprotective effect might be because of its antioxidant and free radical scavenger effects.

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Section: Discussionsupporting

confidence: 82%

Hepatoprotective effect of Barrisal (herbal drug) on carbon tetrachloride induced hepatic damage in rats

Ahmed¹

2013

Afr. J. Pharm. Pharmacol.

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“…SOD catalyzes the superoxide anion into oxygen and hydrogen peroxide, which are later on converted into water by CAT (39). ROS also affects the antioxidant defense mechanism by reducing the intracellular concentration of GPx and decreasing the activity of SOD and CAT (36).…”

Section: Introductionmentioning

confidence: 99%

Protective effects of Vitamin E on CCl4-induced testicular toxicity in male rats

et al. 2016

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The increased generation of free radicals plays an important role in testicular damage. The present study aimed to investigate the adverse effects of carbon tetrachloride (CCl 4 ) on the reproductive system of male rats as well as to examine whether Vitamin E (VE) is able to ameliorate these effects. The rats were equally divided into three groups: control, CCl 4 -treated, and CCl 4 + VE-treated groups. After 4 weeks of treatment, the decrease in body and testes weights, sperm parameters, and the decrease in serum levels of testosterone, luteinizing hormone, and folliclestimulating hormone of CCl 4 -treated rats were ameliorated by VE treatment. The co-administration of VE with CCl 4 significantly decreased the level of lipid peroxidation production (malondialdehyde) and increased the activity of anti-oxidant enzymes (superoxide dismutase and catalase) when compared with the CCl 4 group. Moreover, VE prevented CCl 4 -induced severe testicular histopathological lesions and deformities in spermatogenesis. The results demonstrate that VE augments the anti-oxidants' defense mechanism against CCl 4 -induced reproductive toxicity suggesting a therapeutic role in free radical-mediated infertility.

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“…Although CCl 4 is primarily metabolized in liver, its determinant effect on the brain is well documented (Boer et al, 2009). The lipid solubility of CCl 4 allows it to cross cell membranes and be deposited in different tissues such as the liver, brain and testes (Szymonik-Lesiuk et al, 2003). Free radicals have been implicated in multiple CNS disorders (Kuloglu et al, 2002) because the CNS tissue is highly sensitive to oxidative stress due to its high oxygen consumption, high iron and lipid contents, especially polyunsaturated fatty acids, and the low activity of antioxidant defenses (Carbonell and Rama, 2007).…”

Section: Discussionmentioning

confidence: 99%

“…Animal tissues are constantly coping with highly reactive species, such as superoxide anion, hydroxyl radicals, hydrogen peroxide, and other radicals generated during numerous metabolic reactions (Castillo, 1992;Hartley et al, 1999;Cabre et al, 2000;Melin et al, 2000). It is known that oxidative stress resulting from increased free radical production after CCl 4 intoxication may play a crucial role in the degenerative processes in many tissues of the body (Szymonik-Lesiuk et al, 2003). The cerebellum is a motor structure involved in posture, maintenance of balance, coordination of voluntary movement and motor learning (Alexander et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Impact of liver damage on the histoarchitectural profile of the cerebellar cortex in rats

Da¹,

To²,

Ms³

et al. 2013

Afr. J. Cell. Path

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Aim: This study described the histopathological profile of the cerebellar cortex and liver of rats with carbon tetrachloride (CCl 4 )-induced liver damage. Methods: Male Wistar rats weighing about 250 g were divided into 2 groups (A and B). Group A (control) rats were treated with olive oil (OO) solution only while Group B rats were treated with carbon tetrachloride (CCl 4 ) for 8 weeks. Thereafter, the cerebellum and liver of the rats were processed and stained with cresyl fast violet and H&E, respectively, for histopathological examinations of the cerebellar cortex and liver. Result: Histopathological analysis shows cortical necrosis, uneven neuronal loss with varying range of vacuolations in the cerebellar cortices and moderate vascular congestion and extensive cytoplasmic damage on the hepatocytes of the CCl 4 treated rats. Conclusion: There is of loss of hepatic function in the CCl 4 treated rats.

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Catalase, superoxide dismutase, and glutathione peroxidase activities in various rat tissues after carbon tetrachloride intoxication

Abstract: The results suggest that change in antioxidant enzyme activities may be relevant to the ability of the liver and other investigated organs to cope with oxidative stress during CCl4 poisoning.

Cited by 216 publications

References 31 publications

Hepatoprotective effect of Barrisal (herbal drug) on carbon tetrachloride induced hepatic damage in rats

Hepatoprotective effect of Barrisal (herbal drug) on carbon tetrachloride induced hepatic damage in rats

Protective effects of Vitamin E on CCl4-induced testicular toxicity in male rats

Impact of liver damage on the histoarchitectural profile of the cerebellar cortex in rats

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