Casinga-cheirosa organic extract impairment over Balb-c male mice behavioral phenotype

Estork, Dirce M.; Gusmão, Daniela F.; Paciência, Mateus Luís Barradas; Frana, Sergio Alexandre; Díaz, Ingrit Elida Collantes; Varella, Antonio Drauzio; Younes, Riad Naim; Reis, Luiz F. L.; Montero, Edna Frasson de Souza; Bernardi, Maria Martha; Suffredini, Ivana Barbosa

doi:10.1016/j.bjp.2015.10.004

Cited by 8 publications

(8 citation statements)

References 30 publications

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“…Each plant part has provided an organic and an aqueous extract, made by a 24 h maceration with a 1:1 (v/v) mixture of dichloromethane (DCM) and methanol (MeOH), followed by 24 h maceration with distilled water. 13 Solvents were removed under vacuum or lyophilized. Three hundred milligrams of each extract were weighed in a 5 mL vial and diluted with 3.0 mL of DCM/MeOH or water to obtain a concentration of 100 mg/mL.…”

Section: Extract Preparationmentioning

confidence: 99%

“…7 Our group aimed at the chemical screening of 895 organic and aqueous extracts obtained from plants found in the Amazon rain forest that have been preciously screened for their biological, [8][9][10] pharmacological and toxicological activities. [11][12][13][14][15][16] The extracts were tested for their free radical scavenging and antioxidant activities, as well as screened for the presence of alkaloids, anthraquinones and cardenolides, some of the most active classes of phytochemicals. Table 1; all vouchers are deposited at the Herbarium UNIP.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of Brazilian Plant Extracts as Potential Source of Antioxidant Natural Products Using Bench-Top Assays

Marin

Cavarsan

Díaz

et al. 2018

FRA

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Introduction:The present work reported the antioxidant and chemical screening of Brazilian plant aqueous and organic extracts. Methods: An amount of 895 Brazilian Amazon aqueous and organic plant extracts were tested in thin layer chromatography plates (TLC) using 2, 2-diphenyl-1-picrylhydrazyl (DPPH), β-carotene, Dragendorff's reagent, Kedde's reagent, and KOH reagent so as to evaluate antioxidant activity and chemical profiles. Antioxidant and DPPH free radical scavenging activities results were submitted to chi-square analyses. Results: Only 8.60% of the extracts showed β-carotene/bleaching response, while 96.09% of the extracts responded as a radical scavenger, alkaloids occurred in 8.0% of the extracts whereas anthraquinones occurred in 0.89% of the extracts and cardenolides in 3.89% of the extracts. Conclusion: Present findings described that Amazon plant extracts have a huge potential to be a source of antioxidant compounds to be used in preventive medicine, as well as the chemical screening revealed that their plants can be strategically assessed as a source of alkaloids to be tested in further biological assays.

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Section: Extract Preparationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Analysis of Brazilian Plant Extracts as Potential Source of Antioxidant Natural Products Using Bench-Top Assays

Marin

Cavarsan

Díaz

et al. 2018

FRA

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“…Almond oil as used as vehicle control. [ 12 13 ] Almond oil and the I. P route were chosen due to the absence of bioavailability loss. [ 14 ] Commercial intramuscular diazepam (1 mg/mL; Hipolabor) was used as drug control.…”

Section: Methodsmentioning

confidence: 99%

“…BP can be considered as the emotional, locomotor, fearfulness, and anxiety alterations suffered by laboratory animals exposed to some drug, changing behavior parameters. General behavior activity, sensorial system parameters (auricular reflex, corneal reflex, irritability, response to touch, tail squeeze, and vocal tremor), psychomotor system parameters (body tone, contortion, grip reflex, hindquarter fall, and surface-righting reflex), central nervous system (ataxia, anesthesia, convulsions, hypnosis, tremor, sedation, and straube tail) and autonomous nervous system parameters (lacrimation, breath, ptosis, piloerection, micturition, defecation, hypothermia, and cyanosis) were accessed,[ 8 ] and a score from 0 to 4[ 12 13 14 15 ] was given for each parameter, except to micturition and defecation, that was counted.…”

Section: Methodsmentioning

confidence: 99%

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Mice behavioral phenotype changes after administration of Anani (Symphonia globulifera, clusiaceae), an alternative Latin American and African medicine

et al. 2017

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Background:Anani, (Symphonia globulifera, Clusiaceae), known as chewstick, is a traditional plant occurring in Africa and in Central and South Americas that is used against parasites and microorganisms. Although its use is popular in some of these countries, there is a lack of information related to its influence over behavioral phenotype (BP).Objective:The objective of this study is to evaluate the influence of the administration of the extract obtained from the aerial organs of Anani (EB1257) to male Balb-c mice over BP.Materials and Methods:Open cage observation, open field, and elevated-plus maze apparatuses were used. Evaluations were done 15, 30, 60, 120, and 180 min after intraperitoneal administration of Anani extract.Results:Impairment of general behavior activity, response to touch, tail squeeze, defecation, locomotion and rearing frequency were observed although no signs of hemorrhage or macroscopical alterations of internal organs. Anani is harmful, but not toxic if used in the appropriate doses, yet to be determined to male mice. Impairment of locomotion and defecation was observed, indicating some degree of influence over locomotion, but no alterations in anxiety levels were assessed. Three compounds were previously found in the plant-lupeol (1), β-amyrin (2) and 3-β-hydroxyglutin-5-ene (3), and one is being described for the first time to occur in the species: oleanolic acid (4).Conclusions:The present work contributes in the support of the rational use of Anani, an important Latin American and African alternative medicine, presenting findings that are being reported for the first time.SUMMARY Symphonia globulifera impairs locomotion and defecatin in behavior analysesNo alterations in anxiety was observedOleanolic acid occurs in the species. Abbreviations used: BP: Behavioral phenotype; OF: Open field, EPM: Elevated-plus maze, MMA/ICMBio/SISBIO: Ministério do Meio Ambiente/Instituto Chico Mendes de Conservação da Biodiversidade/Sistema de Autorização e Informação em Biodiversidade, IBAMA/MMA/CGen: Instituto Brasileiro do Meio Ambiente e dos Recursos Naturais Renováveis/Ministério do Meio Ambiente/Conselho de Gestão do Patrimônio Genético, AM: Amazonas State, UNIP: Universidade Paulista, mg: milligram, kg: kilogram, I.P: Intraperitoneal, CEUA/ICS/UNIP: Comissão de Ética no Uso de Animais/Instituto de Ciências da Saúde/Universidade Paulista, LD: Lethal dose, NLD: Nonlethal dose, GBA: General behavior activity, FCHCL3: Fraction chloroform, FBuOH: Fraction buthanol, FH2O: Fraction water, FrHEX: Fraction hexane, FrDCM: Fraction dichloromethane, FrMeOH: Fraction methanol, 13C NMR: Carbon nuclear magnetic resonance, EPA: United States Environmental Protection Agency.

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Luffa operculata fruit aqueous extract induces motor impairments, anxiety-like behavior, and testis damage in rats

Alves

Frias

Kirsten

et al. 2018

Journal of Ethnopharmacology

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Casinga-cheirosa organic extract impairment over Balb-c male mice behavioral phenotype

Cited by 8 publications

References 30 publications

Analysis of Brazilian Plant Extracts as Potential Source of Antioxidant Natural Products Using Bench-Top Assays

Analysis of Brazilian Plant Extracts as Potential Source of Antioxidant Natural Products Using Bench-Top Assays

Mice behavioral phenotype changes after administration of Anani (Symphonia globulifera, clusiaceae), an alternative Latin American and African medicine

Luffa operculata fruit aqueous extract induces motor impairments, anxiety-like behavior, and testis damage in rats

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