Case report: Pregnancy resulting from intracytoplasmic injection of spermatozoa from a frozen-thawed testicular biopsy specimen

Hovatta, Outi; Foudila, Tuija; Siegberg, Rita; Johansson, Kristine; Smitten, Karl von; Reima, Dkka

doi:10.1093/oxfordjournals.humrep.a019140

Cited by 83 publications

(25 citation statements)

References 8 publications

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“…The preserved testicular tissue can be handled in several ways. From these tissues, spermatozoa, spermatocytes and round and elongated spermatids can all be retrieved and used to fertilize oocytes through ICSI (Hovatta et al, 1996;Gianaroli et al, 1999). Testicular tissue can also be transplanted back to the donating individual (autografting), to another individual of the same species (allografting) or to individual of a different species, usually to nude or immunodeficient mice (xenografting).…”

Section: Testicular Tissue Cryopreservationmentioning

confidence: 99%

“…One should also keep in mind that it is very costly to keep immunodeficient mice and handle them under germ-free conditions and, of course, repeated transplantations from one mouse to another are required to maintain viable tissue for many years. Still, testicular tissue cryopreservation was done in several species and pregnancies were achieved in mice (Schlatt et al, 2002b;Shinohara et al, 2002), rabbit (Shinohara et al, 2002), human (Hovatta et al, 1996), Djungarian hamsters (Schlatt et al, 2002b) and marmoset monkeys (Schlatt et al, 2002b), to name a few. Testicular tissue can also be cultured in vitro to give rise to mature and competent cells.…”

Section: Testicular Tissue Cryopreservationmentioning

confidence: 99%

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Genome Banking for Vertebrates Wildlife Conservation

Saragusty¹

2012

Current Frontiers in Cryobiology

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Section: Testicular Tissue Cryopreservationmentioning

confidence: 99%

Section: Testicular Tissue Cryopreservationmentioning

confidence: 99%

Genome Banking for Vertebrates Wildlife Conservation

Saragusty¹

2012

Current Frontiers in Cryobiology

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“…Cryopreservation of testicular tissue has received substantial attention in humans experiencing both obstructive and non-obstructive azoospermia, as well as cancer (Gil-Salom et al 1996;Park et al 2003;Oehninger 2005). Live offspring have been produced following in vitro microinsemination with spermatozoa isolated from cryopreserved murine, rabbit (Shinohara et al 2002) and human testicular tissue (Hovatta et al 1996;Shinohara et al 2002;Park et al 2003) after ICSI. If the cryopreservation of testicular tissue samples could be optimised for different species of wildlife, then testes from every genetically valuable male could be stored until a need to increase that animal's genetic representation in the population arises.…”

Section: Cryopreservation Of Testicular Tissuementioning

confidence: 99%

“…Testicular tissue has been cryopreserved using glycerol (human; Hovatta et al 1996;Shinohara et al 2002) or dimethylsulfoxide (mouse, hamster and marmoset; Schlatt et al 2002b). In brief, this involves equilibrating 0.5-1.0 mm 3 pieces of testicular tissue in a cryoprotectant solution at room temperature, transferring the tissue into cryovials and subjecting it to cryopreservation using a programmable freezer .…”

Section: Cryopreservation Of Testicular Tissuementioning

confidence: 99%

Applications of emerging technologies to the study and conservation of threatened and endangered species

Pukazhenthi

Comizzoli

Travis

et al. 2006

Reprod. Fertil. Dev.

140

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Abstract. Sustaining viable populations of all wildlife species requires the maintenance of habitat, as well as an understanding of the behaviour and physiology of individual species. Despite substantial efforts, there are thousands of species threatened by extinction, often because of complex factors related to politics, social and environmental conditions and economic needs. When species become critically endangered, ex situ recovery programmes that include reproductive scientists are the usual first line of defence. Despite the potential of reproductive technologies for rapidly increasing numbers in such small populations, there are few examples of success. This is not the result of a failure on the part of the technologies per se, but rather is due to a lack of knowledge about the fundamental biology of the species in question, information essential for allowing reproductive technologies to be effective in the production of offspring. In addition, modern conservation concepts correctly emphasise the importance of maintaining heterozygosity to sustain genetic vigour, thereby limiting the practical usefulness of some procedures (such as nuclear transfer). However, because of the goal of maintaining all extant gene diversity and because, inevitably, many species are (or will become) 'critically endangered', it is necessary to explore every avenue for a potential contributory role. There are many 'emerging technologies' emanating from the study of livestock and laboratory animals. We predict that a subset of these may have application to the rescue of valuable genes from individual endangered species and eventually to the genetic management of entire populations or species. The present paper reviews the potential candidate techniques and their potential value (and limitations) to the study and conservation of rare wildlife species.

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“…Live births have been produced using cryopreserved epididymal sperm in many species [10,15]. In addition, intracytoplasmic sperm injection (ICSI) with testicular sperm was successful in the human [6]. Siamese Eld's deer (Rucervus eldii siamensis) are native to Thailand but have been extirpated in the wild and are found only in captivity [12].…”

mentioning

confidence: 99%

A Case Report Concerning Male Gametes Rescued from a Siamese Eld’s Deer (<i>Rucervus eldii siamensis</i>): Post-Thawed Testicular and Epididymal Sperm Quality and Heterologous Zona Pellucida Binding Ability

Thuwanut

Thongphakdee

Sommanustweechai

et al. 2013

The Journal of Veterinary Medical Science

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ABSTRACT. In the present study, the quality of frozen-thawed epididymal and testicular sperm recovered from a Siamese Eld's deer was examined. The epididymal sperm quality was assessed in fresh, cold-stored at 4°C and frozen-thawed samples. Zona binding ability of the frozen-thawed epididymal samples with Burmese Eld's deer oocytes was also evaluated. Testicular sperm extracted from tissues frozen at −80 or −196°C for one month were examined for membrane and DNA integrity. Epididymal sperm retained their quality for up to 24 hr of cold storage at 4°C. The percentages of sperm motility, intact membrane, intact acrosome and intact DNA were 30, 46.5, 27 and 89.5% in the frozen and thawed epididymal sperm, and the average ability to bind with oocytes was 92.5 ± 64 sperm/oocytes. Around 70% of the sperm extracted from testicular tissues cryopreserved at −196 and −80°C for one month showed an intact membrane. In conclusion, epididymal and testicular sperm survived for more than 13 hr post-mortem. Furthermore, cold storage at 4°C and cryopreservation at −196 and −80°C maintain the quality of epididymal and testicular sperm. This study represents a model for male gamete rescue in endangered Eld's deer. KEY WORDS: Siamese Eld's deer, sperm collection post mortem, sperm cryopreservation.doi: 10.1292/jvms.11-0491; J. Vet. Med. Sci. 75(1): 123-125, 2013 Cryotechnology of genetic resources is notable as a potential tool to conserve germplasm of endangered wild species [5]. While ejaculated sperm is recovered from live animals, epididymides and testes are potential major sources of male gametes in animals that die unexpectedly [8]. Live births have been produced using cryopreserved epididymal sperm in many species [10,15]. In addition, intracytoplasmic sperm injection (ICSI) with testicular sperm was successful in the human [6]. Siamese Eld's deer (Rucervus eldii siamensis) are native to Thailand but have been extirpated in the wild and are found only in captivity [12]. Although production of offspring after intrauterine insemination with frozen-thawed ejaculated Burmese Eld's deer sperm was reported [9], there are no studies of Siamese Eld's deer reproductive biology. The aim of this study was to evaluate and cryopreserve male gametes recovered from the epididymis and testes of a Siamese Eld's deer that died in captivity.A two-year-old male Siamese Eld's deer maintained at Dusit Zoo, Bangkok, Thailand, unexpectedly died of an unknown cause at 11:00 pm on January 18, 2009, and was kept in a refrigerator until the next day (10:00 am). The right testis with epididymis was removed, stored in phosphate buffered saline at 4°C and transported to a laboratory within 2 hr. Epididymal sperm was collected by placing the caudal epididymis into 10 ml pre-warmed phosphate buffer saline (PBS), cut into small pieces and incubated at 37°C for 10 min. After epididymal tissues were removed, sperm samples were evaluated for sperm concentration, total number, motility (20× magnification with a phase contrast microscope), morphology (William's...

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Case report: Pregnancy resulting from intracytoplasmic injection of spermatozoa from a frozen-thawed testicular biopsy specimen

Cited by 83 publications

References 8 publications

Genome Banking for Vertebrates Wildlife Conservation

Genome Banking for Vertebrates Wildlife Conservation

Applications of emerging technologies to the study and conservation of threatened and endangered species

A Case Report Concerning Male Gametes Rescued from a Siamese Eld’s Deer (<i>Rucervus eldii siamensis</i>): Post-Thawed Testicular and Epididymal Sperm Quality and Heterologous Zona Pellucida Binding Ability

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