Cascade Reactions in Multicompartmentalized Polymersomes

Peters, Ruud J. R. W.; Marguet, Maïté; Marais, Sébastien; Fraaije, Marco W.; Hest, Jan C. M. van; Lecommandoux, Sébastien

doi:10.1002/ange.201308141

Cited by 191 publications

(157 citation statements)

References 48 publications

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“…Most efforts have focussed on polymersomes [17][18][19] , which lack the biological relevance of lipids, and have a diminished ability for functionalization with biological components (protein pores, receptors, antibodies and so on). They also do not possess the rich phase behaviour that lipids do, which can be taken advantage for the introduction of responsiveness.…”

mentioning

confidence: 99%

Vesicle-based artificial cells as chemical microreactors with spatially segregated reaction pathways

2014

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In the discipline of bottom-up synthetic biology, vesicles define the boundaries of artificial cells and are increasingly being used as biochemical microreactors operating in physiological environments. As the field matures, there is a need to compartmentalize processes in different spatial localities within vesicles, and for these processes to interact with one another. Here we address this by designing and constructing multi-compartment vesicles within which an engineered multi-step enzymatic pathway is carried out. The individual steps are isolated in distinct compartments, and their products traverse into adjacent compartments with the aid of transmembrane protein pores, initiating subsequent steps. Thus, an engineered signalling cascade is recreated in an artificial cellular system. Importantly, by allowing different steps of a chemical pathway to be separated in space, this platform bridges the gap between table-top chemistry and chemistry that is performed within vesicles.

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mentioning

confidence: 99%

Vesicle-based artificial cells as chemical microreactors with spatially segregated reaction pathways

2014

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“…The cellular processes involved during de novo construction of artificial cells include gene expression, protein expression, self-reproduction, nutrient synthesis, and energy conversion, etc. Although it is enormously challenging to mimic natural-like cell, several single steps of these processes have been performed within a celllike compartment such as mimicked protein synthesis [67], replication of genomic RNA [68], designing of cascade reactions [69], and translated chemical messages [70]. However, these different procedures have not been combined yet; the coordination of different cellular processes is still a major challenge.…”

Section: Manipulation Of Motile Microbes: Their Exploitation As Micromentioning

confidence: 99%

Fabrication of nanocomposites and hybrid materials using microbial biotemplates

Shi

Ullah

et al. 2017

Adv Compos Hybrid Mater

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Microbes are important part of life that vary in sizes and shapes, diverse surface chemistry and biology, and porous nature of their cell walls. Besides their importance in industrial processes such as fermentation, these serve as biotemplates and provide a biomimetic approach for fabrication of multifarious complex constructs with predefined features, ordered composites and hybrid nanomaterials, microdevices, and micro/nanorobots through various strategies. The template or building blocks for such approaches can be bacterial, algal, and fungal cells or virus particles. Here, we have summarized recent advancements in biofabrication based on live microbes. Using engineering approaches and suitable methods, live microbes can be manipulated as functional Bmicro/nanodevices and -robots^to further perform biological functions such as replication, distribution, motility, formation of colonies, and secretion of metabolites at will. Biofabrication based on microbes provides effective methods to control and manipulate microbes as functional live building blocks to create micro/nanodevices and -robots for biomedical and energy applications.

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“…Bio-inspired reactions occurring in the confined volume of particles/capsules have been carried out by applying multi-compartment approaches [107,117,125,126]. Advantages of the artificial compartmentalization strategy are the separation of large and small molecules, while at the same time carrying them in one entity with protection from the outside environment, and conduction of cascade reactions [127]. Furthermore, multi-compartment particles/capsules are predicted to be of potential use in theranostic applications where one carrier would perform a dual function: diagnostic and therapeutic [128,129].…”

Section: Anisotropic Capsules and Anisotropic Carriersmentioning

confidence: 99%

Colloidal micro- and nano-particles as templates for polyelectrolyte multilayer capsules

Parakhonskiy

Yashchenok

Konrad

et al. 2014

Advances in Colloid and Interface Science

108

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Colloidal particles play an important role in various areas of material and pharmaceutical sciences, biotechnology, and biomedicine. In this overview we describe micro-and nano-particles used for the preparation of polyelectrolyte multilayer capsules and as drug delivery vehicles. An essential feature of polyelectrolyte multilayer capsule preparations is the ability to adsorb polymeric layers onto colloidal particles or templates followed by dissolution of these templates. The choice of the template is determined by various physico-chemical conditions: solvent needed for dissolution, porosity, aggregation tendency, as well as release of materials from capsules. Historically, the first templates were based on melamine formaldehyde, later evolving towards more elaborate materials such as silica and calcium carbonate. Their advantages and disadvantages are discussed here in comparison to non-particulate templates such as red blood cells. Further steps in this area include development of anisotropic particles, which themselves can serve as delivery carriers. We provide insights into application of particles as drug delivery carriers in comparison to microcapsules templated on them.

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Cascade Reactions in Multicompartmentalized Polymersomes

Cited by 191 publications

References 48 publications

Vesicle-based artificial cells as chemical microreactors with spatially segregated reaction pathways

Vesicle-based artificial cells as chemical microreactors with spatially segregated reaction pathways

Fabrication of nanocomposites and hybrid materials using microbial biotemplates

Colloidal micro- and nano-particles as templates for polyelectrolyte multilayer capsules

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