Carvão ativado no estabelecimento in vitro de cultivares de framboeseira

Fagundes, Cíntia de Moraes; Moreira, Roseane Maidana; Ramm, Aline; Tomaz, Zeni Fonseca Pinto

doi:10.5965/223811711642017406

Cited by 5 publications

(6 citation statements)

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“…In this context, the use of activated charcoal (Me1) or PVP30 (Me2) in the culture medium or exposure to light for Ontogenetic age and inoculation methods for the in vitro establishment of Eucalyptus pilularis Smith Nativa, Sinop, v. 10, n. 1, p. 40-46, 2022. 44 30 days (Me3) reduced the oxidation of E. pilularis tissues. The adsorption of phenolic compounds and toxic substances by activated charcoal during the in vitro culture of woody species has the advantage of reducing tissue oxidation, which can improve and regulate the in vitro growth under certain conditions (FAGUNDES et al, 2017;LENCINA et al, 2018), corroborating the results of the present study. However, few studies have reported its effects according to ontogenetic age.…”

Section: Discussionsupporting

confidence: 89%

“…Regarding the contamination percentages, Fagundes et al (2017) observed that the largest losses due to fungal contamination in Rubus idaeus L. were found in culture medium without the addition of activated charcoal, but the use of antioxidant doses did not promote significant differences. Regarding the number of shoots, in Apuleia leiocarpa, activated charcoal increased the length of shoots and the number of microcuttings per shoot and per micro-stump (LENCINA et al, 2018).…”

Section: Discussionmentioning

confidence: 93%

“…Miranda et al ( 2019), studying the in vitro establishment of Eremanthus incanus, observed that PVP and activated charcoal had non-significant effects on the oxidation percentage of the explants, and values of 50-60% were observed, which were higher than those found in the present study for E. pilularis. In Rubus idaeus L., there was an increase in oxidation in the cultivars as the dose of activated charcoal in the culture medium increased (FAGUNDES et al, 2017). The increase in the concentration of an antioxidant can be harmful by adsorbing other substances from the nutrient medium, causing undesirable effects on in vitro (GALDIANO-JÚNIOR et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…The use of antioxidants in the culture medium, such as activated charcoal and polyvinylpyrrolidone (PVP), and different incubation conditions regarding lighting are ways to reduce tissue oxidation and contamination by microorganisms, making a successful in vitro establishment more likely (FAGUNDES et al, 2017;LENCINA et al;2018;GOLLE et al, 2020). However, there is a need to define the ideal asepsis conditions for each species according to the origin of the tissues.…”

Section: Introductionmentioning

confidence: 99%

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ONTOGENETIC AGE AND INOCULATION METHODS FOR THE IN VITRO ESTABLISHMENT OF Eucalyptus pilularis Smith

Avelar

Moscardini

Souza

et al. 2022

NAT

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We aimed to evaluate the in vitro establishment of nodal segments of Eucalyptus pilularis Smith considering two origins of tissues (Or1 - epicormic shoots collected from pruned branches of selected adult trees; Or2 - shoots collected from seminal mini-stumps) and four inoculation methods (Me1 - culture medium supplemented with 0.5 g L-1 activated charcoal; Me2 - culture medium supplemented with 800 mg L-1 PVP30; Me3 - exposure to light for 30 days; Me4 - exposure to dark for 7 days). At 30 days after the in vitro inoculation of tissues, there was no establishment of tissues from epicormic shoots (Or1). Or2 resulted in lower percentages of tissue oxidation and contamination by microorganisms, in addition to having presented establishment and formation of shoots. Me1 resulted in a lower mean tissue oxidation, although it differed statistically only from Me4. An origin of the tissues of ontogenetic age was a determining factor for the successful in vitro establishment of E. pilularis. The use of the Or2 origin and the Me1, Me2, and Me3 methods are recommended to reduce phenolic oxidation of tissues in the in vitro establishment. Keywords: antioxidant agents; exposure to light; epicormic shoots; tissue oxidation. Idade ontogenética e métodos de inoculação para o estabelecimento in vitro de Eucalyptus pilularis Smith RESUMO: Objetivou-se avaliar o estabelecimento in vitro de segmentos nodais de Eucalyptus pilularis considerando duas origens de tecidos (Or1 - brotos epicórmicos coletados em galhos podados de matrizes adultas e Or2 - brotos coletados de minicepas seminais) e quatro métodos de inoculação (Me1 - meio de cultura suplementado com 0,5 g L-1 de carvão ativado, Me2 - meio de cultura suplementado com 800 mg L-1 de PVP30, Me3 - exposição à luminosidade por trinta dias e Me4 - exposição em ambiente com ausência de luminosidade por sete dias). Aos 30 dias após a inoculação in vitro dos tecidos, constatou-se que não houve estabelecimento de tecidos oriundos da Or1. A utilização de segmentos nodais provenientes da Or2 resultou em menores percentuais de oxidação e de contaminação por microrganismos, além de ter apresentado estabelecimento e emissão de brotos. O uso do Me1 resultou em menor média de oxidação, embora tenha diferido estatisticamente somente do Me4. A origem dos tecidos associada à idade ontogenética foi um fator determinante para o sucesso do estabelecimento in vitro de E. pilularis. Recomenda-se a utilização da Or2 e dos métodos Me1, Me2 e Me3 a fim de reduzir a oxidação fenólica dos tecidos durante o estabelecimento in vitro. Palavras-chave: agentes antioxidantes; exposição à luminosidade; brotos epicórmicos; oxidação de tecidos.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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ONTOGENETIC AGE AND INOCULATION METHODS FOR THE IN VITRO ESTABLISHMENT OF Eucalyptus pilularis Smith

Avelar

Moscardini

Souza

et al. 2022

NAT

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“…Tecidos recém-excisados de espécies lenhosas, principalmente as folhosas, tendem a secretar pigmentos escuros no meio de cultura, sobretudo polifenóis oxidados e taninos, em resposta ao ferimento sofrido (Xavier et al, 2013). Fagundes et al (2017), estudando a adição de carvão ativado ao meio de cultivo de framboeseira, observaram correlação negativa entre oxidação e sobrevivência, o que, segundo os autores, evidenciou que o incremento de oxidação entre os explantes acarreta em redução do percentual de sobrevivência dos mesmos. Para a candeia, o escurecimento do meio de cultura, provavelmente devido à liberação de compostos fenólicos, promove a necrose do material vegetal introduzido.…”

Section: Discussionunclassified

Estabelecimento in vitro de Eremanthus incanus

et al. 2019

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Resumo -Objetivou-se avaliar procedimentos para introdução in vitro de brotações retiradas de mudas de candeia mantidas em viveiro. Para a desinfestação das brotações, testou-se tempos de imersão (5, 10, 15 e 20 min) em hipoclorito de sódio a 2,5% e a influência do uso de fungicida antes (7 e 15 dias) e após a coleta das brotações, nas concentrações de 0,5 e 1,0 g L -1 respectivamente. Para o controle da oxidação dos explantes in vitro, avaliou-se a adição de polivinilpirrolidona (PVP) e carvão ativado ao meio de cultura, ambos nas concentrações 1 e 2 g L -1 . O uso de hipoclorito de sódio permitiu a desinfestação das brotações introduzidas, porém não houve diferença significativa entre os tempos testados. A coleta de brotações 15 dias após a aplicação de fungicida nas mudas fornecedoras das brotações resultou em ausência de contaminação. A oxidação dos explantes atingiu altos níveis, mesmo na presença de PVP e carvão ativado no meio de cultura.Abstract -The objective of this study was to evaluate the procedures for introduction of shoots in vitro taken from seedlings kept in nursery. For the disinfestation of the shoots, immersion times (5, 10, 15 and 20 min) in 2.5% sodium hypochlorite were tested. We also analysed the influence of using fungicide before (7 and 15 days) and after the shoots collection, in the concentrations of 0.5 and 1.0 g L -1 respectively. In order to control the oxidation of explants in vitro, the addition of poly vinyl pyrrolidone (PVP) and activated charcoal to the culture medium, both at concentrations 1 and 2 g L -1 , were evaluated. The use of sodium hypochlorite allowed disinfestation of the introduced shoots, but there was no significant difference between the times tested. The collection of shoots 15 days after the application of fungicide in the seedlings suppliers of the shoots, allowed the absence of contamination. The oxidation of the explants reached high levels even in the presence of PVP and activated charcoal in the culture medium.

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