Caries Inhibition by and Safety of Lactobacillus paracasei DSMZ16671

Tanzer, Jason M.; Thompson, Angela; Lang, Christine; Cooper, Bret; Hareng, Lars; Gamer, Armin; Reindl, A.; Pompejus, Markus

doi:10.1177/0022034510369460

Cited by 43 publications

(29 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, probiotic therapy to prevent or combat oral disease is just evolving, and for example heat-killed bacteria were proven safe, with promising results in animal experiments [Tanzer et al, 2010].…”

Section: Discussionmentioning

confidence: 99%

Cariogenic Effects of Probiotic Lactobacillus rhamnosus GG in a Dental Biofilm Model

Schwendicke

Dörfer²,

Kneist³

et al. 2014

Caries Res

View full text Add to dashboard Cite

Probiotic bacteria have been suggested to inhibit Streptococcus mutans (SM) and thus prevent dental caries. However, supporting evidence is weak and probiotic species might be cariogenic themselves. Thus, we compared and combined the probiotic Lactobacillus rhamnosus GG (LGG) with SM and analysed the resulting mineral loss (ΔZ) in dental tissues. We simulated three biofilm compositions (SM, LGG, SM × LGG), two lesion sites (smooth enamel, dentin cavity) and two nutrition supply frequencies (twice/day, 6 times/day) in a multi-station, continuous-culture biofilm model. A total of 240 bovine enamel and dentin samples were cut, polished and embedded. All experimental procedures were performed in independent duplicates, with 10 samples being allocated to each group for each experiment (final sample size n = 20/group). Biofilms were cultured on the specimens and supplied with 2% sucrose medium and artificial saliva in consecutive pulses. After 10 days, ΔZ and bacterial numbers were assessed. SM × LGG biofilms caused significantly increased ΔZ compared with SM or LGG biofilms (p < 0.01, Mann-Whitney test), and ΔZ was significantly increased in dentin cavities compared with smooth enamel lesions (p < 0.01). Bacterial numbers did not significantly differ between biofilms of different species (p > 0.05, ANOVA). Frequent nutrition supply significantly increased bacterial numbers (p < 0.01). Biofilms in dentin cavities compared to smooth enamel harboured significantly more bacteria (p < 0.05). LGG induced mineral loss especially in dentin cavities and under highly cariogenic conditions. LGG did not have inhibitory effects on SM, but rather contributed to the caries process in vitro.

show abstract

Section: Discussionmentioning

confidence: 99%

Cariogenic Effects of Probiotic Lactobacillus rhamnosus GG in a Dental Biofilm Model

Schwendicke

Dörfer²,

Kneist³

et al. 2014

Caries Res

View full text Add to dashboard Cite

show abstract

“…The possible effect on MS when DNA but not live bacteria was detected is not clear but may be an effect of immune modulation, indicating that non-viable components of probiotic strains can have an influence on the oral microbiota [Taverniti and Guglielmetti, 2011]. Tanzer et al [2010] reported that heat-killed L. paracasei DSMZ16671 sensitively co-aggregated specifically with MS and inhibited colonisation of rat teeth by Streptococcus mutans 10449S, even though the animals were fed with a high-sucrose cariogenic diet. Interestingly, in an in vitro model using the same L. reuteri strains as the present study, alterations in the plaque ecosystem were demonstrated, and L. reuteri persisted and potentially integrated into mature dental biofilms for at least 20 days after cessation of dosing [Madhwani and McBain, 2011], in agreement with our findings.…”

Section: Discussionmentioning

confidence: 99%

Lactobacillus reuteri Influences Regrowth of Mutans Streptococci after Full-Mouth Disinfection: A Double-Blind, Randomised Controlled Trial

Vestman¹,

Hasslöf

Keller

et al. 2013

Caries Res

View full text Add to dashboard Cite

This study assessed whether the persistence of Lactobacillus reuteri DSM 17938 and ATCC PTA 5289 in saliva could delay the regrowth of mutans streptococci (MS) after a full-mouth disinfection with chlorhexidine (CHX). A randomised, double-blind, placebo-controlled study with a 6-week intervention period and 3- and 6-month follow-up was performed. 62 healthy subjects with moderate to high counts of MS were randomly assigned to a test group (n = 32) or a placebo group (n = 30). Before onset of the intervention, subjects received two sessions of professional cleaning, flossing, and application of CHX varnish and rinsed their mouth with a CHX solution between the sessions (2 days). Thereafter, the test group used probiotic lozenges (2/day) containing L. reuteri (DSM 17938 and ATCC PTA 5289; 1 × 10⁸ CFU of each strain), and the placebo group used identical lozenges lacking the lactobacilli. Saliva samples were collected and cultured onto selective media, and isolates of L. reuteri as well as DNA directly extracted from saliva were tested by polymerase chain reaction (PCR) with specific primers. Presence of salivary MS was analysed with a chair-side test. L. reuteri was frequently detected by culture during the intervention period but in only 3 test group subjects at follow-ups. Regrowth of MS statistically significantly differed depending on the presence or absence of L. reuteri DSM 17938 detected by PCR. We conclude that cultivable L. reuteri strains may only sporadically be confirmed after termination of the intervention, but subjects with PCR-detected L. reuteri demonstrated slower regrowth of MS.

show abstract

“…Bacterial coaggregation could provide an explanation for the effect of probiotic bacteria on caries reduction Lang et al, 2010;Tanzer et al, 2010;Holz et al, 2013]. Although coaggregation between LB and SM was tested in our study, no signs of interaction between the species could be detected.…”

Section: Discussionmentioning

confidence: 57%

“…Thus, the tissue composition could determine the differential effect of probiotics relative to the local conditions around the lesions (saturation of the biofilm fluid). An alternative approach for those cases is the use of heat-killed or heatinactivated bacteria, which has demonstrated promising results [Holz et al, 2013;Tanzer et al, 2010].…”

Section: Discussionmentioning

confidence: 99%

Effect of the Probiotic Lactobacillus rhamnosus LB21 on the Cariogenicity of Streptococcus mutans UA159 in a Dual-Species Biofilm Model

et al. 2015

View full text Add to dashboard Cite

Despite promising results using probiotics, evidence of the preventive effect on enamel demineralization is insufficient and the cariogenic potential of probiotics is still controversial. Probiotics could affect biofilm formation and interfere with adherence, growth or coaggregation with Streptococcus mutans in biofilms. However, most of the studies have been conducted using planktonic bacteria. Hence, the aim of the study was to assess the effect of probiotic bacteria on the cariogenicity of S. mutans using an in vitro biofilm caries model on enamel. Single-species biofilms (S. mutans UA159, SM or Lactobacillus rhamnosus LB21, LB) or dual-species biofilms simultaneously inoculated (SM + LB) or LB inoculated 8 h after SM (SM → LB) were grown for 96 h. Biofilms were formed on bovine enamel saliva-coated slabs of known surface hardness (SH) and immersed in culture media. Biofilms were exposed 8 times per day to 10% sucrose. Medium pH was monitored twice daily as a biofilm acidogenicity indicator. After 96 h, biofilms were collected to determine biomass and bacteria viability. Slab demineralization was calculated as percentage of SH loss (%SHL). Additionally, the model was tested with different concentrations of the initial inoculum (10³, 10⁶, 10⁸ cells/ml) and different adhesion times (2 or 8 h). The dual-species biofilm revealed no LB effects on SM cariogenicity, without changes in acidogenicity or %SHL among groups (p > 0.05, n = 12). Lack of activity of LB on SM cariogenicity persisted even when 10⁵ times higher concentration of the probiotic was tested. Coaggregation was not observed. In conclusion, findings suggest that LB does not reduce cariogenicity of SM in a validated experimental caries model.

show abstract

Caries Inhibition by and Safety of Lactobacillus paracasei DSMZ16671

Cited by 43 publications

References 20 publications

Cariogenic Effects of Probiotic <b><i>Lactobacillus rhamnosus GG </i></b>in a Dental Biofilm Model

Cariogenic Effects of Probiotic <b><i>Lactobacillus rhamnosus GG </i></b>in a Dental Biofilm Model

<b><i>Lactobacillus reuteri</i></b> Influences Regrowth of Mutans Streptococci after Full-Mouth Disinfection: A Double-Blind, Randomised Controlled Trial

Effect of the Probiotic <b><i>Lactobacillus rhamnosus</i></b> LB21<b> </b>on the Cariogenicity of <b><i>Streptococcus mutans</i></b> UA159 in a Dual-Species Biofilm Model

Contact Info

Product

Resources

About