Carga proviral do HTLV-1 e HTLV-2: um método simples através da PCR quantitativa em tempo real

Tamegão-Lopes, Bruna Pedroso; Rezende, Priscila Rocha; Maradei-Pereira, Luciana Maria Cunha; Lemos, José Alexandre Rodrigues de

doi:10.1590/s0037-86822006000600007

Cited by 32 publications

(17 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In our study, the presence of HTLV-1/2 was not detected in patients presenting at a tropical/infectious dermatology clinic, which is in contrast to previous reports of associations between HTLV-1/2 and skin diseases 13 . However, it is diffi cult to compare results obtained by studies with distinct study methodologies and on different population samples.…”

contrasting

confidence: 99%

See 1 more Smart Citation

Absence of HTLV-1/2 infection and dermatological diseases in Manaus, State of Amazonas, Brazil

Passos

Moraes

Tamegão-Lopes

et al. 2014

Rev. Soc. Bras. Med. Trop.

View full text Add to dashboard Cite

Introduction:The prevalence of human T-cell lymphotropic virus types 1 and 2 (HTLV-1/2) infection is heterogeneous across different populations. We tested the hypothesis that HTLV-1/2 infection occurs more often in dermatological patients. Methods: A total of 1,091 patients from a tropical dermatology clinic were tested for HTLV-1/2. In parallel, 6865 fi rst-time blood donors from the same geographic area were screened for HTLV-1/2; HTLV-1/2 positive blood donors underwent dermatological examinations. Results: The prevalence of HTLV-1/2 in fi rst-time blood donors was 0.14%. No co-occurrence of HTLV-1/2 infection and dermatological conditions was observed. Conclusions: Our results challenge the hypothesis that HTLV-1/2 infection occurs more often in dermatological patients.Keywords: HTLV-1/2. Dermatology. Association. HTLV and skin diseases.Human T-cell lymphotropic virus (HTLV) was fi rst identifi ed in 1980. It is a member of the Retroviridae family, sub-family Ortothoretroviridae 1 . The global distribution of HTLV is heterogeneous, with high prevalence in Japan, the Caribbean, and South and Central America, and low prevalence in the Middle East and Melanesia 2 . Prevalence studies, performed in blood donors, indigenous populations, and pregnant women in Brazil, suggest that there are an estimated 2 million carriers of HTLV-1/2, and that the distribution varies based on ethnicity, race, and/or population groups 3 . In 1997, a positive test rate for HTLV-1/2 of 0.08% was observed in 2 independent population samples of 1,200 blood donors from Manaus and Florianópolis, and a positive test rate of 1.35% was observed in 1,040 blood donors from Salvador 4 . In a survey of more than 6 million blood donors from 27 Brazilian urban centers, the prevalence of HTLV-1/2 ranged from 0.04% in Florianópolis to 1% in São Luis, with a national average of 0.326% 5. In Manaus, the positive test rate reached 0.53%, which was much higher than was reported in 1997; however, the 2005 study did not confi rm positive enzyme-linked immunosorbent assay (ELISA) test results by Western Blot. In 2003, a study of 11,121 blood donor samples from the State of Acre reported that 12 (0.11%) samples were positive for HTLV: 8 (0.07%) samples were positive for HTLV-1, 2 (0.02%) samples were positive for HTLV-2, and 2 (0.02%) samples were indeterminate6 .An estimated 95% of individuals infected by HTLV remain asymptomatic; however, 2% develop adult T-cell lymphoma/ leukemia (ATLL) and 2-3% present with HTLV-I associated myelopathy/tropical spastic paraparesis (HAM/TSP) 7,8 . In addition, uveitis 9 is associated with HTLV-I. Dermatoses, including infective dermatitis 10 , neoplasia, Norwegian scabies, acquired ichthyosis, and dermatophytosis 11 are associated with HTLV-I infection. These associations suggest that HTVL-I/II may be more prevalent in individuals presenting skin diseases. Interestingly, in the Brazilian State of Minas Gerais, 0.7% of 1,229 patients at a dermatology clinic tested positive for HTLV-1/2, which is 3.3 times higher ...

show abstract

contrasting

confidence: 99%

“…13 . A questionnaire was administered at the time of enrollment in the study and included questions about gender, age, skin color, marital status, and place of origin.…”

mentioning

confidence: 99%

Absence of HTLV-1/2 infection and dermatological diseases in Manaus, State of Amazonas, Brazil

Passos

Moraes

Tamegão-Lopes

et al. 2014

Rev. Soc. Bras. Med. Trop.

View full text Add to dashboard Cite

show abstract

“…Foram utilizadas três sequências alvo: as regiões não homólogas do gene pol do HTLV-1 e HTLV-2, e o gene da albumina como controle endógeno da reação. As sequências dos primers e sondas contidos nos ensaios, assim como o protocolo utilizado, foram descritos segundo Tamegão-Lopes et al 42 .…”

Section: Testes Sorológicosunclassified

Avaliação diagnóstica das infecções por vírus Epstein-Barr, parvovírus B19 e vírus linfotrópico de células T humanas em pacientes portadores de lúpus eritematoso sistêmico em hospital de referência do Estado do Pará, Brasil

Costa¹,

Silva²,

Oliveira³

et al. 2016

Revista Pan-Amazônica de Saúde

View full text Add to dashboard Cite

RESUMOO lúpus eritematoso sistêmico (LES) é uma doença autoimune crônica, cujo desenvolvimento pode estar associado à infecção por vírus, como o vírus Epstein-Barr (EBV), parvovírus B19 e vírus linfotrópico de células T humanas (HTLV). Durante o período de junho a setembro de 2014, foi realizado um estudo transversal, incluindo 85 pacientes oriundos do Hospital Jean Bitar, na Cidade de Belém, Estado do Pará, Brasil. Foi realizada a pesquisa de anticorpos específicos contra os agentes virais estudados, assim como pesquisa da presença do genoma para EBV e HTLV. Foram avaliadas também variáveis clínicas e epidemiológicas. A maioria dos pacientes eram mulheres, tinham média de idade de 30 anos e se declararam brancos. Para EBV, detectou-se positividade de 37,6% para IgM, 98,8% para IgG e 2,4% por qPCR, com quantificações de 85.028 e 298 cópias do genoma/mL de plasma. Para B19, a positividade para IgM foi 0% e para IgG 67,1%. Não houve detecção sorológica ou por qPCR de HTLV. Foi verificada relação estatisticamente significante entre a positividade para IgM anti-EBV e pacientes mais jovens. Esse achado pode estar relacionado com a maior eficiência na produção dessa imunoglobulina nas infecções primárias agudas, que ocorrem geralmente em indivíduos infantes ou adultos jovens. Adicionalmente, o resultado de IgG anti-B19 foi associado à idade avançada dos pacientes, provavelmente por um maior tempo de exposição ao vírus aliado à persistência desse marcador após o contato. A presença dos marcadores não esteve associada às variáveis clínicas e epidemiológicas. Entretanto, o percentual de positividade para o marcador de infecção aguda por EBV pode sugerir um envolvimento do vírus com o LES. Palavras-chave: Lúpus Eritematoso Sistêmico; Vírus Epstein-Barr; Parvovírus B19; HTLV. ARTIGO ORIGINAL | ORIGINAL ARTICLE ABSTRACT Systemic lupus erythematosus (SLE) is a chronic autoimmune disease whose development may be associated with viral infections, such as Epstein-Barr virus (EBV), parvovirus B19, and human T-cell lymphotropic virus (HTLV).A cross-sectional study was performed between June and September 2014 involving 85 patients from the Hospital Jean Bitar, located in Belém, Pará State, Brazil. A survey of specific antibodies against the studied viral agents was conducted, in addition to a survey of the EBV and HTLV genomes. Clinical and epidemiologic variables were also evaluated. Most patients were female, approximately 30 years old, and declared themselves as Caucasians. The following positive results were detected for EBV: IgM = 37.6%, IgG = 98.8%, and qPCR = 2.4%, with 85,028 and 298 copies of the genome per milliliter of plasma. Positive results for B19 were: IgM = 0%, IgG = 67.1%. Serological or qPCR detection did not reveal HTLV. A significant statistical correlation was observed between anti-EBV IgM antibodies and younger patients. These findings may be related to the highly efficient production of this immunoglobulin during acute primary infections, which frequently occurs in children and young adults. Furthermore, t...

show abstract

“…The primers and probes were as previously described 24 . DNA (5μml) was used in the HTLV-1 reaction, presenting a final volume of 25μl consisting of 12.5μl of TaqMan PCR Master Mix (Applied Biosystems, CA, USA), 250nM of HTLV-1F (5'-GAACGCTCTAATGGCATTCTTAAAACC-3'), 250nM of HTLV-1R (5' -GTGGTTGATTGTCCATAGGGCTAT-3') and 200nM of HTLV-1P (5'-FAM-ACAAACCCGACCTACCC-MGB-3') for HTLV-1 pol region amplification; and 150nM of ALB-F (5'-GCTCAACTCCCTATTGCTATCACA-3'), 150nM of ALB-R (5'-GGGCATGACAGGTTTTGCAATATTA-3') and 100nM of ALB-P (5'-VIC-TTGTGGGCTGTAATCAT-MGB-3') for human albumin gene amplification.…”

Section: Real-time Pcr For Htlv-1 Andmentioning

confidence: 99%

Evaluation of the use of real-time PCR for human T cell lymphotropic virus 1 and 2 as a confirmatory test in screening for blood donors

Andrade¹,

Ribeiro²,

Namen-Lopes³

et al. 2010

Rev. Soc. Bras. Med. Trop.

View full text Add to dashboard Cite

Introduction: HTLV-1/2 screening among blood donors commonly utilizes an enzymelinked immunosorbent assay (EIA), followed by a confirmatory method such as Western blot (WB) if the EIA is positive. However, this algorithm yields a high rate of inconclusive results, and is expensive. Methods: Two qualitative real-time PCR assays were developed to detect HTLV-1 and 2, and a total of 318 samples were tested (152 blood donors, 108 asymptomatic carriers, 26 HAM/TSP patients and 30 seronegative individuals). Results: The sensitivity and specificity of PCR in comparison with WB results were 99.4% and 98.5%, respectively. PCR tests were more efficient for identifying the virus type, detecting HTLV-2 infection and defining inconclusive cases. Conclusions: Because real-time PCR is sensitive and practical and costs much less than WB, this technique can be used as a confirmatory test for HTLV in blood banks, as a replacement for WB.

show abstract

Carga proviral do HTLV-1 e HTLV-2: um método simples através da PCR quantitativa em tempo real

Cited by 32 publications

References 29 publications

Absence of HTLV-1/2 infection and dermatological diseases in Manaus, State of Amazonas, Brazil

Absence of HTLV-1/2 infection and dermatological diseases in Manaus, State of Amazonas, Brazil

Avaliação diagnóstica das infecções por vírus Epstein-Barr, parvovírus B19 e vírus linfotrópico de células T humanas em pacientes portadores de lúpus eritematoso sistêmico em hospital de referência do Estado do Pará, Brasil

Evaluation of the use of real-time PCR for human T cell lymphotropic virus 1 and 2 as a confirmatory test in screening for blood donors

Contact Info

Product

Resources

About