Cardiovascular variability and β-ARs gene expression at two stages of doxorubicin – Induced cardiomyopathy

Vasić, Marko; Lončar-Turukalo, Tatjana; Tasić, Tatjana; Matić, Marija; Glumac, Sofija; Bajić, Dragana; Popović, Branka; Japundžić‐Žigon, Nina

doi:10.1016/j.taap.2018.10.015

Cited by 16 publications

(8 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In a study in rats; Expression of β-1AR and β-2AR mRNA in Dilated Cardiomyopathies treated with doxoribucin was evaluated by RT-qPCR and reported no change in β-1AR and β-2AR gene expressions [ 22 ].…”

Section: Discussionmentioning

confidence: 99%

Investigating changes in β-adrenergic gene expression (ADRB1 and ADRB2) in Takotsubo (stress) cardiomyopathy syndrome; a pilot study

et al. 2021

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Investigating changes in β-adrenergic gene expression (ADRB1 and ADRB2) in Takotsubo (stress) cardiomyopathy syndrome; a pilot study

et al. 2021

View full text Add to dashboard Cite

“…Also, despite the use of a similar protocol for DOX-induced cardiomyopathy (cumulative dose 15 mg/kg) by two other studies, spectral analyses of cardiac sympathovagal function were performed 35 and 70 days following the last DOX injection. Lončar-Turukalo, et al 12 reported increased HRV and alterations in LF/HF with increased sympathetic contribution after 35 days, which was lately confirmed by 62 . Also, Vasić’s study reported that LF and LF/HF returned to normal levels after 70-days post DOX treatment.…”

Section: Discussionmentioning

confidence: 86%

Preventive aerobic training preserves sympathovagal function and improves DNA repair capacity of peripheral blood mononuclear cells in rats with cardiomyopathy

Ghignatti

Russo

Becker

et al. 2022

Sci Rep

View full text Add to dashboard Cite

To evaluate the effect of preventive aerobic exercise training on sympathovagal function, cardiac function, and DNA repair capacity in a preclinical model of doxorubicin (DOX)-induced cardiomyopathy. Forty male Wistar-Kyoto rats were allocated into four groups (n = 10/group): D (DOX-treated) and C (controls) remained sedentary, and DT (DOX-trained) and CT (control-trained) performed aerobic training 4 days/week, during 4 weeks before exposure to DOX (4 mg/kg/week during 4 weeks) or saline solution. We evaluated cardiac function (echocardiography), hemodynamic and sympathovagal modulation (artery-femoral cannulation), cardiac troponin T levels, and DNA repair capacity (comet assay). Exercise training preserved ejection fraction (D: − 14.44% vs. DT: − 1.05%, p < 0.001), fractional shortening (D: − 8.96% vs. DT: − 0.27%, p = 0.025) and troponin T levels (D: 6.4 ± 3.6 vs. DT: 2.8 ± 1.7 ng/mL, p = 0.010). DOX increased heart rate variability (C: 27.7 ± 7.9 vs. D: 7.5 ± 2.2 ms2, p < 0.001) and induced sympathovagal dysfunction (LF/HF, C: 0.37 ± 0.15 vs. D: 0.15 ± 0.15, p = 0.036) through exacerbation of sympathetic function (LF, C: 0.22 ± 0.01 vs. D: 0.48 ± 0.24 Hz, p = 0.019). Peripheral mononuclear blood cells of DT animals presented lower residual DNA damage (D: 43.4 ± 8.4% vs. DT: 26 ± 3.4%, p = 0.003 after 1 h). Cardioprotective effects of preventive aerobic exercise training are mediated by preservation of sympathovagal function and improvement of DNA repair capacity of peripheral blood mononuclear cells.

show abstract

Section: Discussionmentioning

confidence: 99%

Investigating Changes in β-Adrenergic Gene Expression (ADRB1 and ADRB2) in Takotsubo (Stress) Cardiomyopathy Syndrome

Onrat

Dural

Yalım

et al. 2021

Preprint

View full text Add to dashboard Cite

Background:Takotsubo Cardiomyopathy (TC) is a rare disorder that is mostly caused by stress and is often misdiagnosed. We aimed to analyze Takotsubo Syndrome at the molecular level by using the Oxford Nanopore Minion Device and its protocol. Methods and Results: Ten patients who were previously diagnosed with Takotsubo Syndrome (increased after decrease in ejection fraction and without critical stenosis in coronary arteries) and 10 healthy individuals in the control group were included in our project. The mean age was 53±12.2 for the patient group and 52.4±9.9 for the control group, and the left ventricular ejection fraction was 50.3±11.5 for the patient group and 64.2±2.8 for the control group (p<0.05). Peripheral blood of patients and healthy individuals was taken and their DNA was obtained. By making long reads throughout the genome, the most studied regions responsible for b- adrenergic signaling pathways; The gene expression level of cardiac β-1 ADRB1(rs1801253-ENST00000369295.4), G>C, (Gly389Arg) and cardiac β-2 ADRB2 (rs1800888-ENSG00000169252), C>T, (Thr165Ile) adrenoceptors was investigated. As a result; no structural variation was detected leading to Takotsubo Cardiomyopathy. The results obtained from the bioinformatics analysis were also checked from the VarSome Tools and similar results were found.Conclusions: Many publications in TC susceptibility have that may lead to adrenergic pathway dysregulation, most studied adrenergic receptor genes in the similar literatures too. We searched for genetic variants in b1AR and b2AR genes in our study and however we could not find any variants in this study, we think larger numbers of cohort studies are needed.

show abstract

Cardiovascular variability and β-ARs gene expression at two stages of doxorubicin – Induced cardiomyopathy

Cited by 16 publications

References 49 publications

Investigating changes in β-adrenergic gene expression (ADRB1 and ADRB2) in Takotsubo (stress) cardiomyopathy syndrome; a pilot study

Investigating changes in β-adrenergic gene expression (ADRB1 and ADRB2) in Takotsubo (stress) cardiomyopathy syndrome; a pilot study

Preventive aerobic training preserves sympathovagal function and improves DNA repair capacity of peripheral blood mononuclear cells in rats with cardiomyopathy

Investigating Changes in β-Adrenergic Gene Expression (ADRB1 and ADRB2) in Takotsubo (Stress) Cardiomyopathy Syndrome

Contact Info

Product

Resources

About