Cardioprotective effect of EGb 761 on myocardial ultrastructure of young and old rat heart and antioxidant status during acute hypoxia

Mozet, Christian; Martin, Roderick; Welt, K; Fitzl, G.

doi:10.1007/bf03324893

Cited by 19 publications

(10 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It should be noted that in many investigations on experimental animals, very high concentrations of EGb 761 (;100 mg/kg) are used orally [54][55][56][57] or intravenously, 58 what is equivalent to 7 g for a 70 kg man, whereas the recommended daily doses of Ginkgo biloba leaves extract for persons with intermittent claudication, cerebrovascular insufficiency, and other disturbances are 30-60 times less. 59 We used much lower GBE concentrations.…”

Section: Discussionmentioning

confidence: 99%

Influence of Ethanol Extract of Ginkgo biloba Leaves on the Isolated Rat Heart Work and Mitochondria Functions

Baliūtytė

Banienė

Gendvilienė³

et al. 2012

Journal of Cardiovascular Pharmacology

View full text Add to dashboard Cite

In this study, we attempted to elucidate whether the effects of ethanol extract of Ginkgo biloba leaves (GBE) observed previously on isolated rat heart mitochondria may be realized in situ (in case of isolated heart perfused under normal conditions and under ischemia-reperfusion). We found that GBE at low concentrations (0.01, 0.05, and 0.1 mL/mL) does not affect the heart rate and parameters of electrocardiogram (ECG) but produces a small increase in the coronary flow. Higher concentration of GBE (0.2 and 0.3 mL/mL) diminished the heart rate, decreased the coronary flow, and tended to enhance the parameters of ECG. The contractility of isolated rat heart and mitochondrial nicotinamide adenine dinucleotide reduced form fluorescence decreased in a GBE concentration-dependent manner. Mitochondria isolated from hearts pre-perfused with GBE (0.05 mL/mL) for 20 minutes before nonflow global ischemia-reperfusion (45 min/15 min) showed higher respiratory rates with pyruvate + malate in state 2 and state 3, higher respiratory control index, and diminished H 2 O 2 generation compared with untreated group. Higher GBE concentration, 0.4 mL/mL, had no effect on H 2 O 2 generation and did not prevent the ischemiareperfusion-induced decrease of pyruvate + malate oxidation in state 3 but even enhanced it. However, in the case of nonischemic perfusions, this GBE concentration had no significant effect on these parameters of respiratory functions of isolated heart mitochondria.

show abstract

Section: Discussionmentioning

confidence: 99%

Influence of Ethanol Extract of Ginkgo biloba Leaves on the Isolated Rat Heart Work and Mitochondria Functions

Baliūtytė

Banienė

Gendvilienė³

et al. 2012

Journal of Cardiovascular Pharmacology

View full text Add to dashboard Cite

show abstract

“…Based on the reduced tolerance of hearts from aged animals to stress conditions, such as inflammation, ischemia-reperfusion, and hypoxia, (39,52,55,65), we hypothesized that chronic alcohol ingestion would exacerbate age-related changes in myocardial structure, function, and protein balance. Indeed, hearts from alcohol-fed aged rats exhibited a relative atrophy, as evidenced by the reduced left ventricular mass and posterior wall thickness compared with control-fed rats of the same age.…”

Section: Discussionmentioning

confidence: 99%

Chronic alcohol consumption disrupts myocardial protein balance and function in aged, but not adult, female F344 rats

Lang

2014

American Journal of Physiology-Regulatory, Integrative and Comp

View full text Add to dashboard Cite

Lang CH, Korzick DH. Chronic alcohol consumption disrupts myocardial protein balance and function in aged, but not adult, female F344 rats. Am J Physiol Regul Integr Comp Physiol 306: R23-R33, 2014. First published November 13, 2013 doi:10.1152/ajpregu.00414.2013.-The purpose of this study was to assess whether the deleterious effect of chronic alcohol consumption differs in adult and aged female rats. To address this aim, adult (4 mo) and aged (18 mo) F344 rats were fed a nutritionally complete liquid diet containing alcohol (36% total calories) or an isocaloric isonitrogenous control diet for 20 wk. Cardiac structure and function, assessed by echocardiography, as well as myocardial protein synthesis and proteolysis did not differ in either alcohol-versus control-fed adult rats or in adult versus aged controlfed rats. In contrast, cardiac function was impaired in alcohol-fed aged rats compared with age-matched control rats. Additionally, alcohol feeding decreased cardiac protein synthesis that was associated with decreased phosphorylation of 4E-BP1 and S6K1. This reduction in mammalian target of rapamycin (mTOR) kinase activity was associated with reduced eIF3f and binding of both Raptor and eIF4G to eIF3. Proteasome activity was increased in alcohol-fed aged rats with a coordinate elevation in the E3 ligases atrogin-1 and muscle RINGfinger protein-1 (MuRF1). These changes were associated with increased regulated in development and DNA damage response 1 (REDD1) and phosphorylation of AMP-activated protein kinase (AMPK) but no increase in AKT or forkhead transcription factor (FOXO)3 phosphorylation. Finally, markers of autophagy (e.g., LC3B, Atg7, Atg12) and TNF-␣ were increased to a greater extent in alcohol-fed aged rats. These data demonstrate that aged female rats exhibit an enhanced sensitivity to alcohol compared with adult animals. Our data are consistent with a model whereby alcohol increases proteolysis via FOXO-independent increase in atrogin-1, which degrades eIF3f and therefore impairs formation of a functional preinitiation complex and protein synthesis.

show abstract

“…The ultrastructure of the myocardium changes with ageing, and this involves alterations at the level of the mitochondria. Whereas some studies demonstrate a reduced number of mitochondria in the cytosol of aged cardiomyocytes, others show that the mitochondrial volume fraction is unaltered during ageing . Mitochondrial shape is altered with increasing age (less elongated and more round), and the area of the mitochondrial inner membrane per mitochondrion is reduced in aged myocardium although cristae configuration is not affected …”

Section: Age‐associated Changes In the Heartmentioning

confidence: 98%

“…Whereas some studies demonstrate a reduced number of mitochondria in the cytosol of aged cardiomyocytes, 4,5 others show that the mitochondrial volume fraction is unaltered during ageing. 6,7 Mitochondrial shape is altered with increasing age (less elongated and more round 8 ), and the area of the mitochondrial inner membrane per mitochondrion is reduced in aged myocardium 9,10 although cristae configuration is not affected. 11 To maintain a pool of healthy mitochondria during ageing, it is important to preserve mitochondrial structure.…”

Section: Mitochondrial Content and Morphology In The Aged Myocardiummentioning

confidence: 99%

Mitochondria and ageing: role in heart, skeletal muscle and adipose tissue

Boengler

Kosiol

Mayr

et al. 2017

J cachexia sarcopenia muscle

311

191

View full text Add to dashboard Cite

Age is the most important risk factor for most diseases. Mitochondria play a central role in bioenergetics and metabolism. In addition, several lines of evidence indicate the impact of mitochondria in lifespan determination and ageing. The best‐known hypothesis to explain ageing is the free radical theory, which proposes that cells, organs, and organisms age because they accumulate reactive oxygen species (ROS) damage over time. Mitochondria play a central role as the principle source of intracellular ROS, which are mainly formed at the level of complex I and III of the respiratory chain. Dysfunctional mitochondria generating less ATP have been observed in various aged organs. Mitochondrial dysfunction comprises different features including reduced mitochondrial content, altered mitochondrial morphology, reduced activity of the complexes of the electron transport chain, opening of the mitochondrial permeability transition pore, and increased ROS formation. Furthermore, abnormalities in mitochondrial quality control or defects in mitochondrial dynamics have also been linked to senescence. Among the tissues affected by mitochondrial dysfunction are those with a high‐energy demand and thus high mitochondrial content. Therefore, the present review focuses on the impact of mitochondria in the ageing process of heart and skeletal muscle. In this article, we review different aspects of mitochondrial dysfunction and discuss potential therapeutic strategies to improve mitochondrial function. Finally, novel aspects of adipose tissue biology and their involvement in the ageing process are discussed.

show abstract

Cardioprotective effect of EGb 761 on myocardial ultrastructure of young and old rat heart and antioxidant status during acute hypoxia

Abstract: Better hypoxia tolerance is demonstrated by the young myocardium, and an obvious hypoxia-protective effect of EGb 761 in both age groups.

Cited by 19 publications

References 28 publications

Influence of Ethanol Extract of Ginkgo biloba Leaves on the Isolated Rat Heart Work and Mitochondria Functions

Influence of Ethanol Extract of Ginkgo biloba Leaves on the Isolated Rat Heart Work and Mitochondria Functions

Chronic alcohol consumption disrupts myocardial protein balance and function in aged, but not adult, female F344 rats

Mitochondria and ageing: role in heart, skeletal muscle and adipose tissue

Contact Info

Product

Resources

About