Carboxylesterase and lipase-catalyzed degradation of phthalate esters in soil and water: Congener structure selectivity and specificity

Sun, Jianqiang; Zhu, Haofeng; Yang, Xindong; Zheng, Yaguang; Sun, Tianchi; Xu, Honglei; Meng, Jiaqi; Zhang, Anping

doi:10.1016/j.eti.2022.102571

Cited by 10 publications

(9 citation statements)

References 37 publications

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Section: Aerobic/anaerobic Biodegradability Endocrine Disturbance And...mentioning

confidence: 85%

“…Catalase (PDB ID: 1P80) [24], nitrate reductase (PDB ID: 1HZV), and esterase (PDB ID: 7CUZ), secreted by aerobic bacteria were selected as the primary degrading enzymes for aerobic degradation [25,26], while hydrolase (PDB ID: 2IOF) [27], oxidoreductase (PDB ID: 1NIC), and alcohol dehydrogenase (PDB ID: 3FMX), secreted by anaerobic bacteria, were selected as the primary degrading enzymes for anaerobic degradation [28,29]. Polyphenol peroxidase (PDB ID: 6TOY) [30], hydrolase (PDB ID: 1VA4) [27], hydrolase (PDB ID: 4PSD), and laccase (PDB ID: 6SOO) [30], which were primarily associated with PAE degradation, were chosen to study the degradation process in soil [20,31]. Du et al identified a composite degradation enzyme (TVSP) consisting of four degradation enzymes using the proteinprotein docking technology to comprehensively evaluate the degradation effect of the four degradation enzymes in soil [32].…”

Section: Screening and Data Source Of Molecular Biodegradable Enzymes...mentioning

confidence: 99%

“…Improving the degradability of PAEs in the soil is, therefore, critical. According to previous research, photodegradation and microbial degradation are the primary mechanisms for PAE degradation in soil [20,55]. Furthermore, two kinds of DEHP substitutes (DEHP-10 and DEHP-12), characterized by high biodegradability, were designed and screened as examples to verify whether they are characterized by high photodegradation and microbial degradation potential in the soil environment.…”

Section: Evaluation and Mechanism Analysis Of The Process Of Photodeg...mentioning

confidence: 99%

“…Among them, microbial degradation is distinguished by its high biosafety. Nonetheless, soil temperature, physical properties, and chemical properties can easily limit the degradation process [20]. The photodegradation of PAEs is primarily achieved in the presence of solar energy and various photosensitive substances in the soil.…”

Section: Introductionmentioning

confidence: 99%

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Molecular Design and Mechanism Analysis of Phthalic Acid Ester Substitutes: Improved Biodegradability in Processes of Sewage Treatment and Soil Remediation

Sun

Zuo

et al. 2022

Toxics

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Phthalic acid esters (PAEs) have the characteristics of environmental persistence. Therefore, improving the biodegradability of PAEs is the key to reducing the extent of ecological harm realized. Firstly, the scoring function values of PAEs docking with various degrading enzymes in sewage treatment were calculated. Based on this, a 3D-quantitative structure-activity relationship (3D-QSAR) model for PAE biodegradability was built, and 38 PAE substitutes were created. By predicting the endocrine-disrupting toxicity and functions of PAE substitutes, two types of PAE substitutes that are easily degraded by microorganisms, have low toxicity, and remain functional were successfully screened. Meanwhile, the differences in the mechanism of molecular degradation difference before and after PAE modification were analyzed based on the distribution characteristics of amino acid residues in the molecular docking complex. Finally, the photodegradability and microbial degradability of the PAE substitutes in the soil environment was evaluated. From the 3D-QSAR model design perspective, the modification mechanism of PAE substitutes suitable for sewage treatment and soil environment degradation was analyzed. We aim to improve the biodegradability of PAEs at the source and provide theoretical support for alleviating the environmental hazards of using PAEs.

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Section: Aerobic/anaerobic Biodegradability Endocrine Disturbance And...mentioning

confidence: 85%

Section: Screening and Data Source Of Molecular Biodegradable Enzymes...mentioning

confidence: 99%

Section: Evaluation and Mechanism Analysis Of The Process Of Photodeg...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Molecular Design and Mechanism Analysis of Phthalic Acid Ester Substitutes: Improved Biodegradability in Processes of Sewage Treatment and Soil Remediation

Sun

Zuo

et al. 2022

Toxics

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“…They can be used in a wide variety of reaction media (aqueous medium, organic solvents [ 17 , 18 ], solvent-free [ 19 ], ionic liquid [ 20 , 21 , 22 ], deep eutectic solvents [ 23 ] and supercritical fluids [ 24 , 25 , 26 , 27 ]) and have a wide substrate specificity, accepting substrates that are very different structurally. However, this is in many instances bound to a high regioselectivity, enantiospecificity and selectivity [ 28 , 29 , 30 , 31 , 32 , 33 , 34 ].…”

Section: Introductionmentioning

confidence: 99%

Tuning Immobilized Commercial Lipase Preparations Features by Simple Treatment with Metallic Phosphate Salts

et al. 2022

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Four commercial immobilized lipases biocatalysts have been submitted to modifications with different metal (zinc, cobalt or copper) phosphates to check the effects of this modification on enzyme features. The lipase preparations were Lipozyme®TL (TLL-IM) (lipase from Thermomyces lanuginose), Lipozyme®435 (L435) (lipase B from Candida antarctica), Lipozyme®RM (RML-IM), and LipuraSelect (LS-IM) (both from lipase from Rhizomucor miehei). The modifications greatly altered enzyme specificity, increasing the activity versus some substrates (e.g., TLL-IM modified with zinc phosphate in hydrolysis of triacetin) while decreasing the activity versus other substrates (the same preparation in activity versus R- or S- methyl mandelate). Enantiospecificity was also drastically altered after these modifications, e.g., LS-IM increased the activity versus the R isomer while decreasing the activity versus the S isomer when treated with copper phosphate. Regarding the enzyme stability, it was significantly improved using octyl-agarose-lipases. Using all these commercial biocatalysts, no significant positive effects were found; in fact, a decrease in enzyme stability was usually detected. The results point towards the possibility of a battery of biocatalysts, including many different metal phosphates and immobilization protocols, being a good opportunity to tune enzyme features, increasing the possibilities of having biocatalysts that may be suitable for a specific process.

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Enzyme‐Catalyzed Biodegradation of Micro‐ and Nanoplastics

Salehipour,

Rezaei,

Motaharian

et al. 2024

Toxic Effects of Micro‐ and Nanoplastics

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Carboxylesterase and lipase-catalyzed degradation of phthalate esters in soil and water: Congener structure selectivity and specificity

Cited by 10 publications

References 37 publications

Molecular Design and Mechanism Analysis of Phthalic Acid Ester Substitutes: Improved Biodegradability in Processes of Sewage Treatment and Soil Remediation

Molecular Design and Mechanism Analysis of Phthalic Acid Ester Substitutes: Improved Biodegradability in Processes of Sewage Treatment and Soil Remediation

Tuning Immobilized Commercial Lipase Preparations Features by Simple Treatment with Metallic Phosphate Salts

Enzyme‐Catalyzed Biodegradation of Micro‐ and Nanoplastics

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