In this study, we describe the synthesis of carbon nanotubes (CNTs) by chemical vapor deposition at 500°C to 975°C, over Co 3 O 4 /MgO catalyst. The size of Co 3 O 4 nanoparticles is controlled by changing cobalt nitrate concentration in aqueous solution by impregnation method. The main object of this study is the synthesis of CNTs that started suddenly without any preheating of catalyst nanoparticles. The effect of sudden initiation and conventional preheating processes of catalyst nanoparticles on yield of CNTs was then described. The results suggest that this sudden initiation of synthesis process affects the carbon yield and morphology of CNTs.