Carbohydrate and alditol analysis by high-performance anion-exchange chromatography coupled with electrochemical detection at a cobalt-modified electrode

Casella, Innocenzo G.; Contursi, Michela

doi:10.1007/s00216-003-1948-4

Cited by 15 publications

(5 citation statements)

References 33 publications

(36 reference statements)

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“…The seed samples of each were milled into powder and (400 mg) of each were later hydrolysed with 6 N HCl for 24 hours and then derivatized with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) to produce stable derivatives. These amino acids are then analysed by reverse phase UPLC [3,8,10,11].…”

Section: Amino Acids Analysismentioning

confidence: 99%

Evaluation of Nutrient Parameters of Selected African Accessions of Bambara Groundnut (Vigna subterranea (L.) Verdc.)

Atoyebi¹,

Osilesi²,

Adebawo³

et al. 2017

AJFN

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Developing countries, especially in Africa are facing challenges of malnourished conditions and food insecurity generally. Nutrients, being components of food ingredient required for survival, normal body function and satisfying the physiological and dietary requirements of the body metabolism [38]. Hence, for meaningful growth and developments in man, the type and amount of nutrients intake and its rate of absorption or level is crucial. However, much emphases and consumption pattern in Africa had been on common or routine food crops like rice, common beans, cassava, maize e.t.c., resulting in the reduced utilisation of other plant species, termed neglected and under-utilised species (NUS), like Bambara groundnut. Selected accessions of Bambara groundnut (Vigna subterranea (L.) Verdc), assembled at the International Institute of Tropical Agriculture, IITA, Ibadan, Nigeria were analysed for important nutrients parameters including moisture content, carbohydrate, protein, crude fibre, total sugar, fat, starch, ash and minerals, based on standard and established protocols at the Crop Utilisation laboratory, IITA, Ibadan, Nigeria. Moreso, samples were sent to the South African grain laboratory, the Willows, South Africa, for the determination of free and essential amino acids using HPLC and UPLC conditions. Results revealed that accessions TVSu -1235, TVSu -1231, TVSu -1205, TVSu -729 and TVSu -1824 could be regarded as high nutrients varieties, while TVSu -1744, TVSu -553 and TVSu -922 are medium nutrients type, while the remaining accessions falls below the medium nutrient range, among these lines of Bambara groundnut evaluated. The protein values has a range of 15.88 ± 0.005 g / 100 g for TVSu -1202 to 24.91 ± 0.011 g / 100 g for TVSu -1231; while their carbohydrate content ranges from 42.77 ± 0.001 g / 100 g for TVSu -1202 to 62.76 ± 0.223 g / 100 g for TVSu -1744.

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Section: Amino Acids Analysismentioning

confidence: 99%

Evaluation of Nutrient Parameters of Selected African Accessions of Bambara Groundnut (Vigna subterranea (L.) Verdc.)

Atoyebi¹,

Osilesi²,

Adebawo³

et al. 2017

AJFN

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“…Based on the separation and detection method, the analytical methods used for monosaccharide composition analysis of polysaccharides include gas chromatography [17], high-performance liquid chromatography (HPLC) [18], ion chromatography (IC) [19], and capillary electrophoresis (CE) [20]. Generally, polysaccharides are difficult to detect by conventional HPLC methods using UV or fluorescence detection because of their nonchromophoric attributes [21]. Electrochemical detection (ECD) provides an alternative detection method due to its simplicity, selectivity and low cost [22].…”

Section: Introductionmentioning

confidence: 99%

Extraction and chemical characterization of novel water-soluble polysaccharides from two palm species and their antioxidant and antitumor activities

Dawood

Elmongy

Negm

et al. 2020

Egyptian Journal of Basic and Applied Sciences

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Two crude heteropolysaccharides were isolated from fruits of Caryota mitis and Chamaerops humilis in percentages of 2.65 and 3.89, respectively. In the case of Caryota mitis (CM) polysaccharide, it consisted of galactose, glucose and mannose as the predominant monosaccharides. While mannose, galactose and arabinose were respectively the predominant monosaccharides in the structure of Chamaerops humilis (CH) crude polysaccharide. Sixteen common amino acids were recognized to be components of the protein-bound polysaccharides of CM and CH. The major amino acid in CM and CH polysaccharides was tyrosine (1.091ng/g) and arginine (0.684 ng/g), respectively. Antioxidant activities of tested polysaccharides were examined by measuring its scavenging ability on 2,2-diphenyl-1-picrylhydrazyl and ferric reducing activity power assay. In a concentration of 1 mg/ml, CM and CH polysaccharides recorded FRAP values of 0.56 and 0.44 µmol Fe ++ /g, respectively. The scavenging activity of CM sample was higher (IC 50 , 422.11 µg/ml) than that of CH polysaccharide (IC 50 , 630.0 µg/ml). Also, the antitumor activity of CM and CH polysaccharides was investigated using HepG2 and MCF-7 human cell lines. The polysaccharide of Caryota mitis had a higher antitumor activity than that of CHPs; it had IC 50 values of 13.6 and 46.4 µg/ml in HepG2 and MCF-7 assays, respectively.

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“…is achieved on GCE, from an aqueous solution of 10 -2 molL -1 NiCl2, acidified with 0.1 mol.L -1 boric acid, by cyclic voltammetry, in the potential range -0.05; -1.05V, (19…”

Section: Electrode Modificationmentioning

confidence: 99%

“…Recent works are more oriented towards oxides of transition metals for example NiOx [11], FeOOH [12], CuO [13], MnO2 [14] , RuO2 [15], Cu2O and Co3O4 [16][17].The presence of oxidized forms provides an intense electro catalytic behaviour, being capable of oxidizing many various organic molecules such as alcohols, amino acids, sugars and nitrites [18][19][20][21][22][23].…”

mentioning

confidence: 99%

Electrochemical Determination of Ethanol, 2- Propanol and 1-Butanol on Glassy Carbon Electrode Modified With Nickel Oxide Film

Benchettara¹

2015

J. Fundam and Appl Sci.

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In this work, we present the modification of a glassy carbon electrode with nickel oxide film which is performed in two successive steps. In the first one, the electrochemical deposition of metallic nickel on the glassy carbon electrode (GCE) is achieved in 0.1M boric acid; in the second step, the metallic deposit is anodically oxidized in 0.1M NaOH. These two operations were carried out in a three electrode cell with a filiform platinum auxiliary electrode, a SCE as potential reference and a working microelectrode of modified glassy carbon with nickel oxides. This electrode is characterized by several electrochemical techniques and is used for the catalytic determination of ethanol, 2-propanol and 1-butanol in 0.1 M NaOH. The proposed chemical mechanism shows that NiO2 acts as a mediator.

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Carbohydrate and alditol analysis by high-performance anion-exchange chromatography coupled with electrochemical detection at a cobalt-modified electrode

Cited by 15 publications

References 33 publications

Evaluation of Nutrient Parameters of Selected African Accessions of Bambara Groundnut (<i>Vigna </i><i>subterranea </i>(L.) Verdc<i>.</i>)

Evaluation of Nutrient Parameters of Selected African Accessions of Bambara Groundnut (<i>Vigna </i><i>subterranea </i>(L.) Verdc<i>.</i>)

Extraction and chemical characterization of novel water-soluble polysaccharides from two palm species and their antioxidant and antitumor activities

Electrochemical Determination of Ethanol, 2- Propanol and 1-Butanol on Glassy Carbon Electrode Modified With Nickel Oxide Film

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