Carbamylated haemoglobin in chronic renal failure

Smith, W. G. J.; Holden, Megan S.; Benton, Michael J.; Brown, C. B.

doi:10.1016/0009-8981(88)90238-0

Cited by 22 publications

(15 citation statements)

References 6 publications

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“…Since then, several studies have demonstrated the accumulation of CDPs in humans, even in healthy individuals. CDPs have been found bound to plasma (17,22,23 ), intracellular (24,25 ), and tissue (26 ) proteins.…”

Section: Rationale For the Involvement Of Protein Carbamylation In Pamentioning

confidence: 99%

“…The concentrations of cHb, expressed as microgram of valine hydantoin (VH) per gram of total hemoglobin (gVH/gHb), range between 27 and 38 gVH/gHb in healthy individuals, and are about 5 times higher in patients with CRF (61,62 ). The increase in cHb correlates with both the duration and the degree of exposure to high blood urea concentrations (25,62 ), suggesting that cHb could be a useful biomarker in differentiating patients with acute or chronic renal failure (63,64 ). More generally, cHb has been described as a biomarker of protein carbamylation, and could constitute an index of uremic balance, used in a similar way as Hb A 1c , which reflects glycemic control in diabetic patients.…”

Section: Available Biomarkers and Their Clinical Relevancementioning

confidence: 99%

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Carbamylation-Derived Products: Bioactive Compounds and Potential Biomarkers in Chronic Renal Failure and Atherosclerosis

2011

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BACKGROUND:Carbamylation is a posttranslational modification of proteins resulting from the nonenzymatic reaction between isocyanic acid and specific free functional groups. This reaction alters protein structural and functional properties and thus contributes to molecular ageing. Many studies have shown the involvement of carbamylated proteins in diseases, especially in chronic renal failure and atherosclerosis.CONTENT: In this review we describe the biochemical basis of the carbamylation process and its role in protein molecular ageing. We summarize the current evidence of protein carbamylation involvement in disease, identify available biomarkers of the carbamylation process and their related analytical methods, and discuss the practical relevance of these biomarkers.

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Section: Rationale For the Involvement Of Protein Carbamylation In Pamentioning

confidence: 99%

Section: Available Biomarkers and Their Clinical Relevancementioning

confidence: 99%

Carbamylation-Derived Products: Bioactive Compounds and Potential Biomarkers in Chronic Renal Failure and Atherosclerosis

2011

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“…There are several papers investigating carbamylated hemoglobin in acute renal failure, nondialyzed chronic renal failure, in patients on regular hemodialysis and on CAPD, in prerenal azotemia and after transplantation [1, 2, 3, 4, 6, 10, 11, 12, 18, 19, 20]. …”

Section: Carbamylated Hemoglobin In Renal Diseasementioning

confidence: 99%

“…Carbamylation of proteins, lipids, peptides and amino acids is widespread in health in mammals [1, 2, 3, 4, 5, 6, 7, 8] and is a natural physiological phenomenon.…”

Section: Introductionmentioning

confidence: 99%

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Carbamylated Proteins and Peptides in Health and in Uremia

Gm¹

1998

Nephron

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Quantification of plasma homocitrulline using hydrophilic interaction liquid chromatography (HILIC) coupled to tandem mass spectrometry

et al. 2011

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Homocitrulline (HCit), an amino acid formed by the carbamylation of ε-amino groups of lysine residues, is considered a promising biomarker for monitoring diseases such as chronic renal failure and atherosclerosis. This paper describes a tandem mass spectrometric method for total, protein-bound and free HCit measurement in plasma samples. HCit was separated from other plasma components by hydrophilic interaction liquid chromatography. Detection was achieved by monitoring transitions of 190.1 > 127.1 and 190.1 > 173.1 for HCit, and 183.1 > 120.2 for d(7)-citrulline used as internal standard. This method allowed HCit quantification within 5.2 min and was precise (inter-assay CV < 5.85%), accurate (mean recoveries ranging from 97% to 106%), and exhibited a good linearity from 10 nmol/L to 1.6 μmol/L. Plasma samples from control and uremic mice (n = 10) were analyzed. In control mice, mean total plasma HCit concentration was 0.78 ± 0.12 μmol/mol amino acids, whereas it was increased 2.7-fold in uremic mice plasma, reaching 2.10 ± 0.50 μmol/mol amino acids (p < 0.001). In conclusion, this method exhibits good analytical performances and meets the criteria of sensitivity suitable for HCit concentration assessment in plasma samples.

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Carbamylated haemoglobin in chronic renal failure

Cited by 22 publications

References 6 publications

Carbamylation-Derived Products: Bioactive Compounds and Potential Biomarkers in Chronic Renal Failure and Atherosclerosis

Carbamylation-Derived Products: Bioactive Compounds and Potential Biomarkers in Chronic Renal Failure and Atherosclerosis

Carbamylated Proteins and Peptides in Health and in Uremia

Quantification of plasma homocitrulline using hydrophilic interaction liquid chromatography (HILIC) coupled to tandem mass spectrometry

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