Carbachol and oxytocin stimulate the generation of inositol phosphates in the guinea pig myometrium

Marc, Sylvie; Leiber, Denis; Harbon, Simone

doi:10.1016/0014-5793(86)80561-0

Cited by 164 publications

(85 citation statements)

References 15 publications

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“…Moreover, m4 receptors have also been shown to mediate Stimulation of phosphoinositide hydrolysis (Peralta et al 1988;Ashkenazi et al 1989). Both second messenger responses have been reported to occur upon muscarinic Stimulation of the immature guinea-pig uterus (Leiber et al 1984;Mare et al 1986). …”

Section: Discussionmentioning

confidence: 99%

Novel pharmacological profile of muscarinic receptors mediating contraction of the guinea-pig uterus

Dörje

Friebe

Tacke

et al. 1990

Naunyn-Schmiedeberg's Arch Pharmacol

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Summary. The present study was designed to further characterize the muscarinic receptors mediating contraction of the guinea-pig uterus. The affinities of various selective muscarinic antagonists were determined and compared with those obtained at M 1 (rabbit vas deferens), M 2 (guinea-pig atria) and M 3 receptors (guinea-pig ileum).The contractile responses of uterine smooth muscle from immature guinea-pigs to carbachol (pD 2 = 5.73)were competitively antagonized by pirenzepine (pA 2 = 7.04), AF-DX 116diazepin-6-one) (pA 2 = 6.96), himbacine (pA 2 = 7.92), methoctramine (pA 2 = 7.52), 4-DAMP (4-diphenylacetoxy-N-methylpiperidine methiodide) (pA 2 = 8.87) and sila-hexocyclium (pA 2 = 8.81). A comparison of affinity values indicates that the muscarinic receptors present in guinea-pig uterus display a novel pharmacological profile which is not consistent with the presence of either an M 1 , M 2 or M 3 receptor. The affinities determined for the different antagonists rather showed a close similarity to those obtained at muscarinic receptors present in rat striatum and NG108-15 cells which are considered pharmacological equivalents (M 4 receptors) of the m4 gene product. We thus hypothesize that the guinea-pig isolated uterus preparation may serve as a simple functional assay system to study the pharmacology of M 4 receptors.

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Section: Discussionmentioning

confidence: 99%

Novel pharmacological profile of muscarinic receptors mediating contraction of the guinea-pig uterus

Dörje

Friebe

Tacke

et al. 1990

Naunyn-Schmiedeberg's Arch Pharmacol

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“…Some of these studies have found that agonist-sensitive PIP2 breakdown is inhibited by pertussis toxin [14,15] and pertussis toxin is believed to exert its effects on metabolism via the inactivation of G proteins. Other studies have found that agonist-sensitive PIP2 breakdown is insensitive to pertussis toxin in several tissues [12,13]. Since PIP2 breakdown in nearly all of the latter tissues is stimulated by either GTP, GTPyS, or by AlF4 -it appears likely that a pertussis toxininsensitive G protein is required to mediate the effects fo agonists on phospholipase C in such tissues.…”

Section: Discussionmentioning

confidence: 93%

“…These experiments indicate that one or more G proteins most likely mediate the action of a contractile agonist on phospholipase C directly. Further evidence in support of this theory comes from the work of those who have shown that the ion AlF4 -can stimulate the breakdown of PIP2 to form IF'3 in Cells from ileum [2O] and myometrium [12], as well as from other tissues [lo]. Some of these studies have found that agonist-sensitive PIP2 breakdown is inhibited by pertussis toxin [14,15] and pertussis toxin is believed to exert its effects on metabolism via the inactivation of G proteins.…”

Section: Discussionmentioning

confidence: 99%

“…Aluminium fluoride (AlF., -) was also shown to stimulate the breakdown of PIP2 in many of these cells [lO,ll]. In most of the cell types studied the agonist-mediated breakdown of PIP2 was not inhibited by pertussis toxin [12,13], although agonist-stimulated phospholipase C was very sensitive to pertussis toxin in a variety of tissues [14,15].…”

Section: Introductionmentioning

confidence: 99%

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G Protein control of inositol lipids in intact vascular smooth muscle

LaBelle

Murray

1990

FEBS Letters

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Agonist-induced PIP, breakdown has been demonstrated in permeabilized vascular smooth muscle and shown to depend on a G protein. Segments of rat tail artery were permeabilized with ATP and EGTA after prelabeling with PHJinositol. Norepinephrine and GTPyS were both able to increase levels of IP, IP, and IP, in the segments. The effects of both norepinephrine and GTPyS on the segments was non-additive. Aluminum fluoride also increased inositol phosphates in intact segments and norepinephrine-stimulated increases in IP, IP, and IP, were insensitive to pertussis toxin.

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“…Adenohypophysis VIb receptors also stimulate phospholipase C. Oxytocin receptors activate phospholipase C and induce an increase in the cytosolic Ca 2+ concentration, which results in strong contractions of the uterus at term (Mirronneau, 1976;Antoni, 1986;Marc et al, 1986). Single amino acids have been found to be important for the activation of this family of receptors.…”

Section: Receptors -Gene and Proteinmentioning

confidence: 99%

Neurohormones: oxytocin, vasopressin and related peptides – structure, genes, receptors, and evolution

Kochman¹

2013

J. Anim. Feed Sci.

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Considered biologically as well as philosophicallyABSTRACT. Oxytocin (OT) and vasopressin (VP), and all related hormones, consist of nine amino acids with cysteine residues in positions 1 and 6 that form a six-amino acid cyclic part, and of a C-terminal glycine in α-amidated form. These neuropeptides are classified into oxytocin and vasopressin families based on the amino acid residue at position 8. OT-like and VP-like peptides are present in every vertebrate species. These peptides are a very ancient family of hormones having representatives in diverse species of invertebrates. Invertebrates have either a vasopressin-family peptide or an oxytocin-family peptide, whereas bony fishes, the ancestors of land vertebrates, have both isotocin and vasotocin. Presently, two evolutionary structural lineages have been proposed: an isotocin-mesotocin-OT line, associated with reproductive functions, and a vasotocin-VP line participating in water homeostasis. The ancestral gene encoding the precursor protein has been present in the animal genome for a period exceeding 500 million years of evolution. The exceptionally high stability of this structure of nine-amino acid peptides during the entire process of evolution suggests very powerful selective pressure, possibly by evolution together with respective receptors and specific processing enzymes. A novel gene with a distinct function and expression appeared during evolution through duplication of an ancestral gene. The synteny and order of genes in the neurohypophysial hormone gene locus are conserved in the lamprey, elephant shark, coelacanth, and tetrapods, but disrupted in teleost fishes presumably due to the rearrangements facilitated by a whole-genome duplication event in the teleost fish ancestor. 284 Evolution of neurohormones

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Carbachol and oxytocin stimulate the generation of inositol phosphates in the guinea pig myometrium

Cited by 164 publications

References 15 publications

Novel pharmacological profile of muscarinic receptors mediating contraction of the guinea-pig uterus

Novel pharmacological profile of muscarinic receptors mediating contraction of the guinea-pig uterus

G Protein control of inositol lipids in intact vascular smooth muscle

Neurohormones: oxytocin, vasopressin and related peptides – structure, genes, receptors, and evolution

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