Caracterización molecular de cepas de Escherichia coli aisladas de productos lácteos artesanales elaborados en Mérida, Venezuela

Guillén, Leidys; Millán, Beatriz; Araque, Marı́a

doi:10.1016/j.infect.2014.04.004

Cited by 5 publications

(8 citation statements)

References 19 publications

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“…On the other hand, 21 isolates (17.95%), two obtained from milk samples and the others from cheese, were resistant to two antimicrobial classes. These results were similar to the findings described by Guillén et al (2014), which detected multiresistance in 11.1% of E. coli isolates obtained from artisanal dairy products manufactured in Venezuela. Ribeiro et al (2016) and Ombarak et al (2018) reported multiresistance rates lower than 20% in E. coli isolates collected from raw milk and/or cheese produced with raw milk in Brazil and Egypt, respectively.…”

Section: Resultssupporting

confidence: 92%

“…Not only the identification of virulence factors in E. coli, but also the investigation of antimicrobial susceptibility profile, are of utmost importance, since these microorganisms are often involved in serious human infections (SLAMA et al, 2010) and, bacterial dissemination is considered highly associated with processing production, transformation, manufacturing and trade of food (KIRBIS; KRIZMAN, 2015). Studies worldwide have demonstrated serious concern about the alarming antimicrobial resistance index, and the number of multi-drug resistant E. coli strains isolated from raw and pasteurized milk (NTULI et al, 2016), dairy products (GUILLÉN et al, 2014;NOBILI et al, 2016) and other types of food (GÓMEZ-ALDAPA et al, 2016;WANG et al, 2017). One of the major resistance mechanisms developed by E. coli to adapt to antibiotic is the hydrolysis of the β-lactam ring through enzymes, defined as extended-spectrum beta-lactamases (ESBL), that confer resistance to antimicrobials from β-lactam class, including penicillins, cephalosporins and monobactams (ECDC, 2013;PFEIFER et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Detection of virulence genes and antimicrobial resistance profiles of Escherichia coli isolates from raw milk and artisanal cheese in Southern Brazil

Parussolo

Sfaciotte

Dalmina

et al. 2019

SCA

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The serrano artisanal cheese is a typical product from South region of Brazil, which is produced by skilled cheesemakers using raw milk. The contamination of this food by Escherichia coli has a great impact on public health, since it could threat the consumers’ health. The study evaluated the presence of virulence genes, antimicrobial susceptibility profiles and bofilm-production ability of Escherichia coli isolates obtained from raw milk and artisanal cheese produced in Southern Brazil. A total of 117 isolates of E. coli were characterized by multiplex PCR to detect the following virulence genes: eae for enteropatogenic E. coli (EPEC), lt and st for enterotoxigenic E. coli (ETEC), stx for shiga toxin-producing E. coli (STEC), stx and eae for enterohemorrhagic E. coli (EHEC), ipaH for enteroinvasive E. coli (EIEC) and aggR for enteroaggregative E. coli (EAEC). In addition, antimicrobial susceptibility profile to 22 antimicrobial agents was also performed by disk diffusion method, and we searched for extended-spectrum beta-lactamases (ESBL) and/or carbapenemase- producing isolates. Isolates that were positive for ESBL and carbapenemase were further investigated for the presence of the genes: blaTEM, blaSHV, blaOXA, blaCTX-M, for ESBL and blaOXA-48 for carbapenemase. Further, isolates had their ability to form biofilms investigated by the red Congo agar method. Virulence genes of E. coli were identified in 21.37% of the tested isolates, which were classified as EPEC (the most prevalent pathotype) and ETEC or EAEC. Ten (8.55%) of the total studied E. coli isolates revealed a multidrug-resistant profile, since they were resistant to three or more antimicrobial classes; whereas four isolates (3.42%) were classified as ESBL-producers and showed the presence of blaTEM gene. None of the isolates exhibited carbapenemase activity nor did they carry carbapenemase genes. From the total of E. coli isolates, 79 (67.52%) were considered potential biofilm producers. These results address a serious public health issue, since artisanal cheeses pose a risk to consumers’ health, since may be sources of dissemination of diarrheogenic E. coli, that can cause from subclinical to severe and fatal infections in children and adults, and also emphasize the need to improve adaptations/adjustments in the manufacturing processes of these products.

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Section: Resultssupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Detection of virulence genes and antimicrobial resistance profiles of Escherichia coli isolates from raw milk and artisanal cheese in Southern Brazil

Parussolo

Sfaciotte

Dalmina

et al. 2019

SCA

View full text Add to dashboard Cite

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“…Although CTX-M-15 has spread pandemically in humans, this enzyme has recently been reported in food-producing animals in South America [ 29 ]. In this regard, previous studies examining the presence of ESBL-PE in dairy products and meats marketed in Venezuela have determined the impact of food products both as reservoirs, and dissemination mechanisms of these strains [ 12 , 13 , 30 ].…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Fecal Carriage of CTX-M-15 Beta-Lactamase-Producing <italic>Escherichia coli</italic> in Healthy Children from a Rural Andean Village in Venezuela

Araque¹,

Labrador²

2018

Osong Public Health Res Perspect

Self Cite

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ObjectivesAntimicrobial resistant extended-spectrum-β-lactamase-producing Enterobacteriaceae (ESBL-PE) have been shown to be present in healthy communities. This study examined healthy children from the rural Andean village of Llano del Hato, Mérida, Venezuela, who have had little or no antibiotic exposure to determine the prevalence of fecal carriage of ESBL-producing Escherichia coli (ESBL-EC).MethodsA total of 78 fecal samples were collected in healthy children aged from 1 to 5 years. ESBL-EC were selected in MacConkey agar plates with cefotaxime and further confirmed by the VITEK 2 system. ESBL were phenotypically detected and presence of bla genes and their variants were confirmed by molecular assays. Determination of phylogenetic groups was performed by PCR amplification. Risk factors associated with fecal carriage of ESBL-EC-positive isolates were analyzed using standard statistical methods.ResultsOf the 78 children studied, 27 (34.6%) carried ESBL-EC. All strains harbored the blaCTX-M-15 allele. Of these, 8 were co-producers of blaTEM-1, blaTEM-5, blaSHV-5 or blaSHV-12. Co-resistance to aminoglycosides and/or fluoroquinolones was observed in 9 strains. 51.9% of ESBL-EC isolates were classified within phylogroup A. A significant, positive correlation was found between age (≥2.5 – ≤5 years), food consumption patterns and ESBL-EC fecal carriage.ConclusionThis is the first study describing the high prevalence of fecal carriage of ESBL-EC expressing CTX-M-15- among very young, healthy children from a rural Andean village in Venezuela with scarce antibiotic exposure, underlining the importance of this population as a reservoir.

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“…On the other hand, studies conducted in dairy products have shown that 73.3% (33/45) of the E. coli strains isolated were susceptible to all antibiotics tested and 24.4% (11/45) showed resistance to ampicillin. The phylogenetic analysis of the E. coli isolates resulted in grouping into two phylogroups, A and B1, which have a higher frequency of resistance genes than those that were grouped in B2 and D. It is worth to notice that E. coli isolates in this study that belonged to phylogroup A and B1 were commensal strains with few or no virulence factors [43]. These results suggest that the food chain is the vehicle for the transfer of resistant genes, and it has been suggested that E. coli strains present in food are the original carrier of many mechanisms of antibiotic resistance in the intestinal microbiota of humans.…”

Section: Overview Of Resistant Pathogens Isolated From Foodmentioning

confidence: 67%

Multidrug-Resistant Bacterial Foodborne Pathogens: Impact on Human Health and Economy

Mancilla-Becerra¹,

Lías-Macías²,

Ramírez-Jiménez³

et al. 2020

Pathogenic Bacteria

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The drug abuse known to occur during growth of animals intended for food production, because of their use as either a prophylactic or therapeutic treatment, promotes the emergence of bacterial drug resistance. It has been reported that at least 25% of the foodborne isolates show drug resistance to one or more classes of antimicrobials (FAO 2018). There are diverse mechanisms that promote drug resistance. It is known that the use of sub-therapeutic doses of antibiotics in animals intended for food production promotes mutations of some chromosomal genes such as gyrA-parC and mphA, which are responsible for quinolone and azithromycin resistance, respectively. Also, the horizontal transfer of resistance genes as groups ("cassettes") or plasmids makes the spread of resistance to different bacterial genera possible, among which there could be pathogens. The World Health Organization considers the emergence of multidrug-resistant pathogenic bacteria as a health problem, since the illnesses caused by them complicate the treatment and increase the morbidity and mortality rates. The complication in the illness treatment caused by a multidrug-resistant pathogen causes economic losses to patients for the payment of long stays in hospitals and also causes economic losses to companies due to the absenteeism of their workers.

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Caracterización molecular de cepas de Escherichia coli aisladas de productos lácteos artesanales elaborados en Mérida, Venezuela

Cited by 5 publications

References 19 publications

Detection of virulence genes and antimicrobial resistance profiles of Escherichia coli isolates from raw milk and artisanal cheese in Southern Brazil

Detection of virulence genes and antimicrobial resistance profiles of Escherichia coli isolates from raw milk and artisanal cheese in Southern Brazil

Prevalence of Fecal Carriage of CTX-M-15 Beta-Lactamase-Producing <italic>Escherichia coli</italic> in Healthy Children from a Rural Andean Village in Venezuela

Multidrug-Resistant Bacterial Foodborne Pathogens: Impact on Human Health and Economy

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Caracterización molecular de cepas de Escherichia coli aisladas de productos lácteos artesanales elaborados en Mérida, Venezuela

Cited by 5 publications

References 19 publications

Detection of virulence genes and antimicrobial resistance profiles of Escherichia coli isolates from raw milk and artisanal cheese in Southern Brazil

Detection of virulence genes and antimicrobial resistance profiles of Escherichia coli isolates from raw milk and artisanal cheese in Southern Brazil

Prevalence of Fecal Carriage of CTX-M-15 Beta-Lactamase-Producing &lt;italic&gt;Escherichia coli&lt;/italic&gt; in Healthy Children from a Rural Andean Village in Venezuela

Multidrug-Resistant Bacterial Foodborne Pathogens: Impact on Human Health and Economy

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Prevalence of Fecal Carriage of CTX-M-15 Beta-Lactamase-Producing <italic>Escherichia coli</italic> in Healthy Children from a Rural Andean Village in Venezuela