Caracterização de um isolado do Pepper mild mottle virus que não quebra a resistência do gene L3 em Capsicum sp.

Abstract

RESUMO ABSTRACT Characterization of a non-L3 gene-resistance breaking Pepper mild mottle virus isolate in CapsicumA strain of Pepper mild mottle virus (PMMoV) was identified by biological and serological analysis of pepper (Capsicum baccatum) 'Dedo de Moça' seeds from São Paulo, SP, Brazil. To confirm these results, RT-PCR was performed with specific primers flanking the coat protein (CP) ORF of tobamoviruses in subgroup 1. The DNA-amplified fragments, when sequenced and compared with other tobamovirus CP sequences stored in the GenBank, showed nucleotide similarity between 94 to 100% with other PMMoV CP sequences, and below 75% with the subgroup I tobamovirus species (Tobacco mosaic virus, Tomato mosaic virus and Odontoglossum ringspot virus) and 65% with species in the subgroups II and III. The Brazilian isolate showed 100% identity with a Japanese isolate, strongly suggesting that this pathogen could have been introduced from that country. This fact confirms the importance of the isolate's characterization, in order to establish adequate control measures to guarantee healthy seed interchange and to avoid the introduction and spread of tobamoviruses into new growing areas. The deduced CP aminoacids sequence showed that the PMMoV-BR was not capable to overcome L3 gene resistance. This fact was confirmed by the inoculation and reaction of differential Capsicum species. The Brazilian isolate was not able to infect C. chinense (L3) and C. chacoense (L4). Do gênero

Procedure for detecting tobamovirus in tomato and pepper seeds decreases the cost analysis

“…The virus species of Tobamovirus genus have worldwide distribution and can occur in different environments, in tropical as well as in temperate climates. In Brazil the most frequently found of tobamoviruses species are Tobacco mosaic virus (TMV), type species of the genus; Tomato mosaic virus (ToMV), which is present in solanaceous crops, including vegetable and ornamentals plants; and Pepper mild mottle virus (PMMoV CP) in pepper (Moreira et al 2003;Eiras et al 2004;Duarte et al 2007;Cezar et al 2009;Silva et al 2008;2011).…”

“…The simplest, called the biological method, consists of planting the seeds and observing the symptoms in infected plants, which also can be analyzed by other diagnostic methods. Serological methods such as the DAS and the PTAS ELISA have been extensively used for the detection of several species of virus with a satisfactory result (Chitra et al 1999;Eiras et al 2004;Sevik and Kose-Tohumcu 2011). Among the molecular methods, the most widely used is the RT-PCR (Eiras et al 2004;Lee et al 2004;Kumar et al 2011;Liu et al 2014).…”

“…Serological methods such as the DAS and the PTAS ELISA have been extensively used for the detection of several species of virus with a satisfactory result (Chitra et al 1999;Eiras et al 2004;Sevik and Kose-Tohumcu 2011). Among the molecular methods, the most widely used is the RT-PCR (Eiras et al 2004;Lee et al 2004;Kumar et al 2011;Liu et al 2014). A combination of serologic and molecular methods, called IC-RT-PCR, has also been widely used (Berniak et al 2009;Mulholland 2009;Chikh-Ali and Karasev 2015).…”