volume 1, issue 1, P100002 2020
DOI: 10.1016/j.xpro.2019.100002
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Abstract: SUMMARY Nucleoside-containing metabolites such as the oxidized and reduced forms of nicotinamide adenine dinucleotide (NAD + and NADH), 3′-desphospho-coenzyme A (dpCoA), and flavin adenine dinucleotide (FAD) can be incorporated as RNA 5′ end caps by serving as non-canonical initiating nucleotides (NCINs) for transcription initiation by RNA polymerase. We recently reported ″CapZyme-seq,″ a 5′-RNA-seq method that enables the differential detection and quantitation of relative yi…

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