Capsiate administration results in an uncoupling protein-3 downregulation, an enhanced muscle oxidative capacity and a decreased abdominal fat content in vivo

Abstract

Objectives: The involvement of skeletal muscle mitochondrial uncoupling protein-3 (UCP3) in the control of energy expenditure in skeletal muscle and at the whole-body level is still a matter of debate. We previously reported that UCP3 downregulation is linked to an enhanced mitochondrial energy metabolism in rat skeletal muscle as a result of acute capsiate treatment. Here, we aimed at investigating noninvasively the effects of chronic capsiate ingestion on metabolic changes occurring in exercising gastrocnemius muscle and at the whole-body level. Methods: We used an original experimental setup allowing a complete noninvasive investigation of gastrocnemius muscle function in situ using 31-phosphorus magnetic resonance spectroscopy. Whole-body fat composition was determined using magnetic resonance imaging and UCP3 gene expression was measured by quantitative real-time RT-PCR analysis. Results: We found that a 14-day daily administration of capsiate (100 mg kg À1 body weight) reduced UCP3 gene expression and increased phosphocreatine level at baseline and during the stimulation period in gastrocnemius muscle. During muscle stimulation, pH i showed a larger alkalosis in the capsiate group suggesting a lower glycolysis and a compensatory higher aerobic contribution to ATP production. Although the capsiate-treated rats were hyperphagic as compared to control animals, they showed a lower weight gain coupled to a decreased abdominal fat content. Conclusion: Overall, our data indicated that capsiate administration contributes to the enhancement of aerobic ATP production and the reduction of body fat content coupled to a UCP3 gene downregulation.

Role of Thermo-Sensitive Transient Receptor Potential Channels in Brown Adipose Tissue

“…69,70) Capsinoids were also demonstrated to enhance metabolic rate and energy expenditure in WT mice, but not in TRPV1KO mice. In addition, capsiate administration contributes to enhancement of aerobic ATP production and reduction of body fat content, 71) and also up-regulates UCP1 and UCP2 expression. 72,73) Mice given the TRPV1 agonist monoacylglycerol showed increases in UCP1 expression levels in brown adipocytes and suppressed accumulation of visceral fat in mice fed HFD and sucrose.…”

Evaluation of green pepper (Capsicum annuum L.) juice on the weight gain and changes in lipid profile in C57BL/6 mice fed a high-fat diet

“…13,38,40 Our results are similar to those obtained in a study by Faraut et al in which the administration of capsiate, an ingredient of Capsicum annuum L., resulted in a lower weight gain coupled to a decreased abdominal fat content, as determined by MRI analysis, although the capsiate-treated rats were hyperphagic compared to control animals. 41 Most of the excess energy is stored in the form of triglycerides in adipose tissue, and increased adipose tissue mass arises through hypertrophy and hyperplasia. 42 The current study showed that it was assumed that the administration of green pepper juice decreased the adipose tissue expandability, although not significantly.…”

Identification of a Pharmacological Inducer of Lipoic Acid Synthase that Impacts Mitochondrial Function: Metabolic Benefits and Body Weight Loss without Changes in Caloric Intake

“…Our approach is not without precedent since there have been other reports in the literature demonstrating the feasibility of achieving weight reduction without energy intake reduction [27][28][29][30]. Calculation of the polar surface area of compound 1 revealed that this area is too large for it to cross the blood brain barrier (supplementary table).…”