Capillary electrophoresis of the collagen crosslinks HP and LP utilizing absorbance, wavelength-resolved laser-induced fluorescence and conventional fluorescence detection

In vitro model for endogenous optical signatures of collagen

“…Collagen cross-link fluorescence typically exhibits a peak between 380 and 400 nm when excited at 320 nm. 10 This is consistent with fluorescence from LP and HP cross-links, spectra that appear to be consistent with the prepolymerized gel illustrated in Fig. 3͑a͒.…”

“…The excitation wavelength ex = 320 nm was chosen because this is the wavelength of maximal excitation of collagen cross-links HP and LP. 10 Both 270 and 360 nm were chosen because they Fig. 1 Schematic of in vitro imaging system with quartz imaging chamber and media flow-through.…”

Capillary electromigration methods for the study of collagen

“…Other interesting collagenous substances are crosslinks. Veraat et al [44] developed a capillary electrophoretic method for the determination of typical collagen crosslinks hydroxylysylpyridinoline (HP), and lysylpyridinoline (LP). This method uses a frequency-doubled Rhodamine dye laser at excitation frequencies of 290 and 325 nm.…”

Hyphenated chromatographic methods for biomaterials

“…Increasing the path length as in bubble cell capillaries can increase sensitivity but this modification often affects peak resolution. Therefore, when highly sensitive analyses of biological or environmental samples are desired, it is common to employ other more sensitive detection techniques such as MS or LIF (Tomlinson et al, 1994b;Veraart et al, 1998;Blaschke, 1999b, Takeda et al, 1999) with DAD serving as an effective screening method for rapid analyses and experimental simplicity.…”