Capillary electrophoresis‐laser‐induced fluorescence detection of rat brain catecholamines with microwave‐assisted derivatization

Liu, Wanling; Hsu, Yi-Fen; Liu, Yuwei; Singco, Brenda; Chen, Ssu-Wei; Huang, Hsi‐Ya; Chin, Ting‐Yu

doi:10.1002/elps.201200262

Cited by 14 publications

(12 citation statements)

References 24 publications

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“…Meanwhile, since most amino acids lacked UV‐visible absorption, using a CE system coupled to LIF as detector would be more suitable for amino acids analysis because of higher sensitivity (nM–pM levels) and specificity, however the derivatization procedure is often time consuming and the availability of fluorescent reagent is limited . In our previous report, we have successfully conjugated catecholamines with FITC (a common fluorescence dye) via microwave irradiation within 3 min . Herein, the isothiocyanate moiety of FITC reacts readily with amino acid via nucleophilic attack on the amine group with the aid of microwave heating, thereby producing FITC‐tagged amino acid molecules, making it more hydrophobic than its original form.…”

Section: Performance Of the Proposed Microwave‐assisted Derivatizatiomentioning

confidence: 81%

Determination of amino acids by microemulsion electrokinetic chromatography laser induced fluorescence method

et al. 2014

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In this study, detection of 20 FITC-derivatized amino acids using an MEEKC-LIF was demonstrated. In order to achieve good separation for hydrophobic amino acids, the MEEKC method was employed and detection limits were obtained in the range of 0.32-2.2 nM, which is comparable to previous reports on amino acid analyses. Furthermore, a significant reduction in the reaction time from 1 h for conventional derivatization to 3 min for the microwave-assisted derivatization was observed and achieved, as opposed to the traditional pretreatment of real sample due to its complexity prior to the analysis of amino acid content. Finally, this microwave-assisted derivatization MEEKC-LIF method successfully determined amino acids in beverage, food, and biological samples (rat brain) with good recovery.

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Section: Performance Of the Proposed Microwave‐assisted Derivatizatiomentioning

confidence: 81%

Determination of amino acids by microemulsion electrokinetic chromatography laser induced fluorescence method

et al. 2014

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“…Therefore, to develop a quick derivatization method for AAs was necessary for the determination of these compounds. Microwave‐assisted derivatization could significantly reduce the derivatization time for AAs . Microwave power was a crucial factor that affected the derivatization.…”

Section: Resultsmentioning

confidence: 99%

Study of the effect of culture mediums on the amino acid metabolites for Corynebacterium glutamicum using high‐speed micellar electrokinetic chromatography

et al. 2019

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Corynebacterium glutamicum (C. glutamicum) is a well‐known workhorse for the industrial production of amino acids. Different carbon, nitrogen, and sulfur source may force the bacterium to produce specific metabolites. In this work, a method of high‐speed MEKC with LIF detection was developed to rapidly analyze the amino acid metabolites released by C. glutamicum, which is fed with different culture mediums. Corynebacterium glutamicum was cultured in microbial fuel cells to monitor its metabolism process and collect its metabolites. In the CE system, a microliter‐scale sample reservoir was designed and applied to perform tiny volume sample injection. With the assistance of microwave, the derivatization time for amino acids with FITC was greatly shortened to 6 min. Under the optimized condition, the eight candidate amino acids of metabolites could be rapidly separated within 2 min. The whole analysis process for real samples, from sampling to determination, could be shortened to less than 10 min. The results showed that C. glutamicum could produce additional l‐lysine and l‐valine as the metabolites when fed with glucose and l‐methionine, respectively. The method proved that culture mediums used to feed C. glutamicum had great effect on the bacterium's metabolites.

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“…In addition, rapid separations are feasible with CE because high voltages can be applied to short capillaries and separation efficiency is not dependent on column length. To identify biological and pharmaceutical analysis, CE is coupled to a variety of detectors, including fluorescence, mass spectrometry, and electrochemical detection [22][23][24] .…”

Section: Capillary Electrophoresis Methodmentioning

confidence: 99%

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2017

IJPSR

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Norepinephrine (NE) is an important catecholamine neurotransmitter that acts as a biochemical messenger in central nervous systems and neuronal cells of mammals, which is secreted and released by the adrenal medulla and the noradrenergic neurons during the synaptic transmission. It is often used for treating bronchial asthma, myocardial infarction hypertension and organic heart disease. Abnormal release of NE will contribute to the occurrence of many nerve related diseases, such as paraganglioma, ganglia neuroblastoma, ganglion neuronal, and Parkinson's disease. In addition, increased NE concentration in plasma is associated with coronary heart disease and with muscle sympathetic nerve traffic in human obesity. Furthermore, NE is involved in the occurrence and development of cardiac hypertrophy through the activation of myocardial adrenergic receptors on cell membranes in myocardial tissue. Therefore, the rapid, sensitive, and selective detection of NE level is significant for monitoring physiological function and diagnosing diseases. In this article the studies of detection methods for NE in recent years are reviewed.

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Capillary electrophoresis‐laser‐induced fluorescence detection of rat brain catecholamines with microwave‐assisted derivatization

Cited by 14 publications

References 24 publications

Determination of amino acids by microemulsion electrokinetic chromatography laser induced fluorescence method

Determination of amino acids by microemulsion electrokinetic chromatography laser induced fluorescence method

Study of the effect of culture mediums on the amino acid metabolites for Corynebacterium glutamicum using high‐speed micellar electrokinetic chromatography

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