Increasing bone formation on the surfaces of implants such as screws,p lates, or shims holdsg reat significance for clinical medicine. However,o steogenesisi mplant coatings that mimic natural bone in terms of both their components and structuralf eatures are still lacking. Here we report the biomimetic interface of calcium phosphate (CaP) in ac ollagen matrix fabricatedb yc ontrolled mineralization that presentsb iomimetic porous features. The porousC aP/ collageni nterface, with at hickness of about1mm, significantly enhances osteogenesis,a sv erifieda tb oth the gene and protein levelsa sw ell as by in vivo experiments. Ta king advantage of the generality of the method, the biomimetic interfacew as prepared on av ariety of substrates, including conductive substrates, 3D metal meshes, plastic or elastic substrates, and even on filter papers. The adjustability and generality of the methodh ave enabled new characterization tests to be developed during experimentso nc ells and thus should greatly facilitate clinicalm edicine and tissue engineering.Supporting information and the ORCID identification number(s) for the author(s) of this articlecan be found under: https://doi.Figure 5. a) Schematic illustration of the animal experiments. Alizarin Red Ss taining of b) normal tissue, c) the tissuearound blank Ti,a nd d) the tissuearound CaP@(COL/PEG) 15 @Ti. Microscopic observations of the tissueslices containinge )blank Ti,f)CaP@(COL/PEG) 15 @Ti, and g) magnificationo ft he image of (f) after in vivo cell culturef or 4weeksinaBALB/c mice model. The new bonematrix is indicated by toluidine blue (TB, blue) staining(the area below the dotted line). Scale bar,2 00 mm.