Canine limb homotransplantation

Goldwyn, Robert M.; Beach, P M; Feldman, David R.; Wilson, Ruth

doi:10.1097/00007890-196611000-00025

Cited by 6 publications

(6 citation statements)

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“…These studies were performed before the advent of CSA, and most animals died from toxicity to drugs used to prevent rejection. 22,23 Unlike humans, dogs are acutely sensitive to tacrolimus, limiting the usefulness of this model in the study of modern immunotherapy regimens. 24,25 Studies using non-human primates showed return of motor and sensory function to the CTA.…”

Section: Discussionmentioning

confidence: 99%

Osteomyocutaneous flap as a preclinical composite tissue allograft: Swine model

et al. 2000

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Composite tissue allotransplantation (CTA) constitutes one of the last frontiers of microsurgery. Prior to its clinical application, the long-term efficacy of modern immunotherapy must be tested in a pre-clinical CTA model. Based on the concept of osteomyocutaneous forearm flap, we developed a CTA flap model in swine. After identifying the vascular territory of the flaps in six pigs (vascular casting), flaps were transplanted from mismatched donor to recipient pigs (n = 6). Rejection was assessed daily by visual inspection and histopathology of biopsy specimens. Recipient pigs were able to ambulate immediately following surgery. There were no flap failures owing to technical or surgical complications. Rejection occurred over a period of 7 days as manifested by edema, cellular infiltration, epidermalysis, and thrombosis. This pre-clinical flap model is excellent for evaluating the effectiveness of modern immunotherapy because it is anatomically and immunologically relevant and because the minimal morbidity caused to the animal permits long-term studies.

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Section: Discussionmentioning

confidence: 99%

Osteomyocutaneous flap as a preclinical composite tissue allograft: Swine model

et al. 2000

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“…Despite a relative decrease in immunogenicity when transplanted within a vascularized limb allograft, skin remains the principal target of the recipient's immune system. This was first reported by Goldwyn et al (10) in a canine limb transplantation model and was further documented by the group of the Massachusetts General Hospital in an experimental model of composite tissue transplantation between major histocompatibility complex‐matched, minor antigen mismatched miniature swine. Indeed, while indefinite survival of the musculoskeletal part of the graft was achievable in this model, skin component was rejected by the recipients (11) even when frozen donor skin graft was placed long after acceptance of the musculoskeletal graft (12).…”

Section: Immunogenicity Of Parts Versus Immunogenicity Of Whole: Lessmentioning

confidence: 70%

Composite Tissue Allograft Extends a Helping Hand to Transplant Immunologists

Thaunat

Badet²,

Eljaafari

et al. 2006

American Journal of Transplantation

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The first successful human hand transplantation, performed on September 1998, has translated the scope of 'composite tissue allotransplantation' from research concepts into clinical practice. Beyond microsurgical problems that have been overcome several years ago, the main obstacle that still prevents the generalization of composite tissue allotransplantation is immunologic.This review, which summarizes the evidence obtained both from experimental animal models and from the first recipients of a hand transplant, is focused on the two immunological characteristics of composite allografts that set them apart from other solid organ allografts: (i) they contain skin tissue that elicits a strong immune response; and (ii) they contain lymphoid tissues (such as bone marrow and lymph nodes) that have the potential both to attack the recipient, and also to down-modulate the host immune response and induce tolerance.While on one hand, the composite tissue allografts raise new challenges to transplant immunologists, on the other they provide answers to questions that have remained unresolved for a long time. In this sense, composite tissue allografts extend a helping hand to transplant immunologists.

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“…It was the original model used for limb transplantation by Goldwyn et al in 1966. 27 Our canine model, in particular, has several advantages that make it ideal for developing CTA protocols. The dogs are outbred, leading to a diversity of MHC antigens available for research.…”

Section: Discussionmentioning

confidence: 99%

A Preclinical Canine Model for Composite Tissue Transplantation

Mathes

Noland

Graves

et al. 2010

J reconstr Microsurg

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The feasibility of composite tissue allografts (CTA) has been demonstrated by the successful transplantation of the hand, abdomen and face. However, the survival of these transplants is dependent on immunosupression. Our laboratory is interested in achieving tolerance in order to decrease the risks associated with the use of chronic immunosuppression. The purpose of this experiment was to develop a large animal model for CTA. Four canine flaps were auto-transplanted to examine the use of a myocutaneous rectus flap based on the deep inferior epigastric vessels. Five CTA transplants were performed between Dog Leukocyte Antigen (DLA) identical littermates without any therapy. The allografts were followed clinically and underwent routine biopsies. The anatomic dissections and auto-transplants were all successful and revealed that the flap could be divided into two separate components. Skin is routinely perfused by the superficial epigastric artery. Rectus muscle is perfused by the deep inferior epigastrc system. This allows the allografts to be transplanted as muscle, skin or with both components based on the external iliac artery and veins. The DLA-identical littermates rejected the allografts in 15 to 30 days. This study demonstrates the versatility of the myocutaneous rectus flap for use in canines as composite tissue allograft models.

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Canine limb homotransplantation

Cited by 6 publications

References 0 publications

Osteomyocutaneous flap as a preclinical composite tissue allograft: Swine model

Osteomyocutaneous flap as a preclinical composite tissue allograft: Swine model

Composite Tissue Allograft Extends a Helping Hand to Transplant Immunologists

A Preclinical Canine Model for Composite Tissue Transplantation

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