‘Candidatus Phytoplasma noviguineense’, a novel taxon associated with Bogia coconut syndrome and banana wilt disease on the island of New Guinea

Miyazaki, Akio; Shigaki, Toshiro; Koinuma, Hiroaki; Iwabuchi, Nozomu; Rauka, G. B.; Kembu, A.; Saul, Josephine; Watanabe, Kinzo; Nijo, Takamichi; Maejima, Kensaku; Yamaji, Yutaka

doi:10.1099/ijsem.0.002480

Cited by 41 publications

(19 citation statements)

References 20 publications

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“…4). 30–38) Each phytoplasma was also found to have two rDNA operons. 39) When closely related phytoplasmas within the same ‘ Candidatus ’ species are compared, housekeeping gene sequences are used in addition to those of 16S rDNA sequences for finer molecular characterization.…”

Section: Molecular Detection and Classification Of Mlosmentioning

confidence: 99%

Molecular and biological properties of phytoplasmas

Namba

2019

Proc. Jpn. Acad., Ser. B

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Phytoplasmas, a large group of plant-pathogenic, phloem-inhabiting bacteria were discovered by Japanese scientists in 1967. They are transmitted from plant to plant by phloemfeeding insect hosts and cause a variety of symptoms and considerable damage in more than 1,000 plant species. In the first quarter century following the discovery of phytoplasmas, their tiny cell size and the difficulty in culturing them hampered their biological classification and restricted research to ecological studies such as detection by electron microscopy and identification of insect vectors. In the 1990s, however, tremendous advances in molecular biology and related technologies encouraged investigation of phytoplasmas at the molecular level. In the last quarter century, molecular biology has revealed important properties of phytoplasmas. This review summarizes the history and current status of phytoplasma research, focusing on their discovery, molecular classification, diagnosis of phytoplasma diseases, reductive evolution of their genomes, characteristic features of their plasmids, molecular mechanisms of insect transmission, virulence factors, and chemotherapy.

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Section: Molecular Detection and Classification Of Mlosmentioning

confidence: 99%

Molecular and biological properties of phytoplasmas

Namba

2019

Proc. Jpn. Acad., Ser. B

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“…Moreover, based on 16S rRNA, phylogeny phytoplasmas were classified into 43 provisional species in the genus ‘ Candidatus ( Ca .) Phytoplasma’ (Miyazaki et al., 2018). For finer strains differentiation, other molecular markers are used, for example genes coding ribosomal proteins S19, L22, S3 ( rps19 , rpl22 , rps3 ), protein translocase subunit ( secY ) or translational elongation factor Tu ( tuf ; Katanić, Krstin, Jezić, Zebec, & Ćurković‐Perica, 2016; Makarova et al., 2012).…”

Section: Introductionmentioning

confidence: 99%

Artemisia vulgaris, a new host of 16SrV‐C phytoplasma related strains infecting black alder in Poland

Jurga

Zwolińska

2020

Journal of Phytopathology

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Yellowing of leaf tissue and strongly deformed shoots were observed in common mugwort (Artemisia vulgaris L.) growing in a nature reserve in Southern Poland. Similar foliage chlorosis together with abnormal shoot proliferation was noticed on alder tree (Alnus glutinosa Gaertn.) growing next to the common mugwort. DNA specific fragments coding 16S rRNA and ribosomal proteins (rp) were amplified from mugwort and alder samples using direct and nested PCR (Polymerase Chain Reaction) assays. Phylogenetic relationships inferred from 16S and rps3 genes indicated that strains infecting mugwort and alder were most closely related to phytoplasmas of subgroups 16SrV‐C and 16SrV‐D. Based on the restriction fragment length polymorphism (RFLP) analysis of the 16S rDNA, the investigated phytoplasma strains were classified to subgroup 16SrV‐C. Two sequence variants of the rps3 gene which differed by a single nucleotide were detected in all analysed samples by pairwise analysis of the aligned reads. Taking into account that this single‐nucleotide polymorphism (SNP) occurs among 16SrV‐C and 16SrV‐D related phytoplasmas and that the phytoplasmas have a single copy of rp operon, we concluded that each plant species was infected by two distinct, closely related phytoplasma strains. To the best of our knowledge, this is the first report of group 16SrV‐C related phytoplasmas infecting common mugwort worldwide, adding a new host species that is possibly linked to the spread of the alder pathogen in Eastern Europe. Although alder yellows phytoplasma has been frequently found in Europe, this is the first detection of phytoplasmas associated with alder in Poland.

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“…Molecular methods using the highly conserved 16S rRNA gene as well as other conserved biomarkers, including ribosomal protein, tuf, secA, and secY genes, have been used for the detection, differentiation, and classification of phytoplasmas (Lee, Gundersen-Rindal, Davis, & Batoszyk, 1998b;Seemüller, Marcone, Lauer, Ragozzino, & Göschl, 1998;Marcone, Lee, Davis, Ragozzino, & Seemüller, 2000;Hodgetts, Boonham, Mumford, Harrison, & Dickinson, 2008). Based on molecular classification and the guidelines established by the IRPCM Phytoplasma/Spiroplasma Working Team-Phytoplasma Taxonomy Group (IRPCM, 2004), phytoplasmas have been classified into 43 'Candidatus Phytoplasma' species (Arneodo, et al, 2007;Fernández, Galdeano, Kornowski, Arneado, & Conci, 2016;Miyazaki et al, 2017;Naderali et al, 2017).…”

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Catharanthus roseus (Apocynaceae) naturally infected with diverse phytoplasmas in Costa Rica

Villalobos

Bottner-Parker

Lee

et al. 2019

RBT

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Phytoplasmas (class Mollicutes) are causal agents of plant diseases with an economic impact on crops or threatening local biodiversity. A survey was conducted from 2012 to 2016 on infected Catharanthus roseus plants that exhibited symptoms reminiscent of phytoplasma infection throughout Costa Rica. A total of 73 plants were collected exhibiting symptoms such as virescence, phyllody, axillary proliferation, little leaf, leaf malformation, chlorosis, or yellowing. All samples were tested by nested PCR using phytoplasma universal and specific primer pairs. Phytoplasma infection was detected in 52 (71.2 %) of the plants collected. Phytoplasmas of six subgroups belonging to 16Sr groups I, III, IX, XIII and XV were identified based on sequencing and in silico RFLP analyses. ´Candidatus Phytoplasma asteris´ (16SrI) was the predominant group among the positive samples (n = 30) showing variety of symptoms and wide distribution from sea level to ca. 1 400 masl in six of the seven Costa Rican provinces. Group 16SrIII was the second most abundant (14 samples); and the remaining three groups were seldom found in C. roseus (8 samples). Moreover, group 16SrXIII phytoplasma was detected for the first time in the country. To the best of our knowledge, this is the first report of natural infection of C. roseus with phytoplasma subgroups 16SrI-B, 16SrI-P, 16SrIII-F, 16SrIX-F, 16SrXIII-A, and 16SrXV-B in Costa Rica and Central America.

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‘Candidatus Phytoplasma noviguineense’, a novel taxon associated with Bogia coconut syndrome and banana wilt disease on the island of New Guinea

Cited by 41 publications

References 20 publications

Molecular and biological properties of phytoplasmas

Molecular and biological properties of phytoplasmas

Artemisia vulgaris, a new host of 16SrV‐C phytoplasma related strains infecting black alder in Poland

Catharanthus roseus (Apocynaceae) naturally infected with diverse phytoplasmas in Costa Rica

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