Candidate metabolites for methane mitigation in the forage legume biserrula

Ghamkhar, Kioumars; Rochfort, Simone; Banik, Bidhyut Kumar; Revell, Clinton

doi:10.1007/s13593-018-0510-x

Cited by 6 publications

(13 citation statements)

References 27 publications

(36 reference statements)

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“…Ts is of interest for multiple economically significant traits. It exhibits variation for suppression of methanogenesis in the rumen ( Kaur et al., 2017a ; Ghamkhar et al., 2018 ), which genome information can help further elucidate. Genome data may also offer insight into adaptive variation to edaphic stress in forage legumes.…”

Section: Discussionmentioning

confidence: 99%

An improved reference genome for Trifolium subterraneum L. provides insight into molecular diversity and intra-specific phylogeny

et al. 2023

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Subterranean clover (Trifolium subterraneum L., Ts) is a geocarpic, self-fertile annual forage legume with a compact diploid genome (n = x = 8, 544 Mb/1C). Its resilience and climate adaptivity have made it an economically important species in Mediterranean and temperate zones. Using the cultivar Daliak, we generated higher resolution sequence data, created a new genome assembly TSUd_3.0, and conducted molecular diversity analysis for copy number variant (CNV) and single-nucleotide polymorphism (SNP) among 36 cultivars. TSUd_3.0 substantively improves prior genome assemblies with new Hi-C and long-read sequence data, covering 531 Mb, containing 41,979 annotated genes and generating a 94.4% BUSCO score. Comparative genomic analysis among select members of the tribe Trifolieae indicated TSUd 3.0 corrects six assembly-error inversion/duplications and confirmed phylogenetic relationships. Its synteny with T. pratense, T. repens, Medicago truncatula and Lotus japonicus genomes were assessed, with the more distantly related T. repens and M. truncatula showing higher levels of co-linearity with Ts than between Ts and its close relative T. pratense. Resequencing of 36 cultivars discovered 7,789,537 SNPs subsequently used for genomic diversity assessment and sequence-based clustering. Heterozygosity estimates ranged from 1% to 21% within the 36 cultivars and may be influenced by admixture. Phylogenetic analysis supported subspecific genetic structure, although it indicates four or five groups, rather than the three recognized subspecies. Furthermore, there were incidences where cultivars characterized as belonging to a particular subspecies clustered with another subspecies when using genomic data. These outcomes suggest that further investigation of Ts sub-specific classification using molecular and morpho-physiological data is needed to clarify these relationships. This upgraded reference genome, complemented with comprehensive sequence diversity analysis of 36 cultivars, provides a platform for future gene functional analysis of key traits, and genome-based breeding strategies for climate adaptation and agronomic performance. Pangenome analysis, more in-depth intra-specific phylogenomic analysis using the Ts core collection, and functional genetic and genomic studies are needed to further augment knowledge of Trifolium genomes.

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Section: Discussionmentioning

confidence: 99%

An improved reference genome for Trifolium subterraneum L. provides insight into molecular diversity and intra-specific phylogeny

et al. 2023

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“…Thus, along with the variation in the type of substrates, mode of application and inclusion levels, the biological differences that exist among the species, varieties and accessions of Moringa also play a significant role in the CH 4 inhibition obtained in several studies. However, previous studies did not indicate the direct effects and variation of varieties, ecotypes, cultivars, individual plants and plant parts of Moringa on antimethanogenic potential and digestibility, the different antimicrobial activities reported among the mentioned factors [ 16 , 44 ]. In the current study, none of the Moringa accession leaf extracts negatively affected the feed digestion characteristics and kinetics of fermentation.…”

Section: Discussionmentioning

confidence: 99%

“…Blanks were used to correct the total gas and CH 4 produced by the inoculums. Finally, the total gas and the CH 4 produced within 48 h of incubation from the substrate feed treated with leaf extracts of the accessions were expressed as mL/g DM incubated feed [ 44 ].…”

Section: Methodsmentioning

confidence: 99%

Relationships between Agronomic Traits of Moringa Accessions and In Vitro Gas Production Characteristics of a Test Feed Incubated with or without Moringa Plant Leaf Extracts

Zeru

Hassen²,

Apostolides³

et al. 2022

Plants

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The use of medicinal plants and their extracts has recently attracted the attention of many researchers as a methane (CH4) mitigation strategy. This study evaluated the relationship of agronomic traits of Moringa accessions with in vitro gas production measurements and feed digestibility from ruminants. Twelve Moringa accessions were grown at the Roodeplaat experimental site of the Agricultural Research Council in Pretoria, South Africa. Agronomic traits, such as seedling survival rate, leaf yield, canopy and stem diameter, plant height, number of primary branches, plant vigor, greenness, chlorosis, disease and pest incidences were recorded. The leaves were harvested in the fifth month after transplanting to the field. Freeze-dried leaves were extracted with methanol, and their total phenolic and total flavonoid contents were determined. The extract was applied at a dose of 50 mg/kg of dry matter (DM) feed for in vitro gas production studies. Most of the growth and agronomic traits, i.e., seedling survival rate, leaf yield, canopy diameter, plant height, number of primary branches, the score of plant vigor, and greenness, total phenolics and flavonoids were significantly different among the accessions except for stem diameter and chlorosis score. All accession leaf extracts significantly reduced the total gas and CH4 production compared with the control with equal or higher in vitro organic matter digestibility. Higher CH4 inhibition was obtained in Moringa oleifera (M. oleifera) A3 (28.4%) and A11 (29.1%), whereas a lower inhibition was recorded in A1 (17.9%) and A2 (18.2%). The total phenolic (0.62) and total flavonoid (0.71) contents as well as most agronomic traits of the accessions were positively correlated with the CH4 inhibition potential of the accessions. Moringa oleifera accessions A3, A8 and A11 resulted in higher in vitro CH4 inhibition potential and improved organic matter digestibility of the feed with equal or higher adaptability performances in the field. Thus, there is a possibility of selecting Moringa accessions for higher antimethanogenic activity without compromising the feed digestibility by selecting for higher total phenolics, total flavonoids and agronomic performances traits. There is a need for further study to determine the long-term adaptability of promising accessions in the study area with concurrent antimethanogenesis efficacy when used in the diet of ruminant animals.

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“…Next, the CH 4 concentration was converted to ml by multiplying the total gas produced (mL) with the percentage of CH 4 in the sample as CH 4 (mL) = total gas produced (mL) × % CH 4 concentration. Eventually, the antimethanogenic potential of the plant extract was expressed as a mL/g DM incubated feed [46]. In addition, methane inhibition potential was converted to a percentage compared with the control, and the accessions were classified into lower and higher methane inhibition groups.…”

Section: Determination Of In Vitro Total Gas Methane and Organic Matt...mentioning

confidence: 99%

“…These active metabolites can also be used as a marker for the selection of commercial varieties that will be used as a source of plant extracts to mitigate enteric methane from ruminants. However, many studies did not describe this aspect of medicinal plants and their components at the metabolite level, except for some that showed the use of crude plant extracts [16,[44][45][46]. Therefore, this study intended to establish the relationship be-tween secondary plant metabolites' m/z ion-features with the in vitro methane inhibition characteristics of Moringa accessions.…”

Section: Introductionmentioning

confidence: 99%

Screening of Candidate Bioactive Secondary Plant Metabolite Ion-Features from Moringa oleifera Accessions Associated with High and Low Enteric Methane Inhibition from Ruminants

et al. 2022

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This study evaluated the relationship of secondary bioactive plant metabolite ion-features (MIFs) of Moringa oleifera accessions with antimethanogenesis to identify potential MIFs that were responsible for high and low methane inhibition from ruminants. Plant extracts from 12 Moringa accessions were evaluated at a 50 mg/kg DM feed for gas production and methane inhibition. Subsequently, the accessions were classified into low and high enteric methane inhibition groups. Four of twelve accessions (two the lowest and two the highest methane inhibitors), were used to characterize them in terms of MIFs. A total of 24 samples (12 from lower and 12 from higher methane inhibitors) were selected according to their methane inhibition potential, which ranged from 18% to 29%. Ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) and untargeted metabolomics with univariate and multivariate statistical analysis with MetaboAnalyst were used in the study. Although 86 MIFs showed (p < 0.05) variation between higher and lower methane inhibition groups and lay within the detection ranges of the UPLC-MS column, only 14 were significant with the volcano plot. However, Bonferroni correction reduced the candidate MIFs to 10, and their R2-value with methane production ranged from 0.39 to 0.64. Eventually, MIFs 4.44_609.1462 and MIF 4.53_433.1112 were identified as bioactive MIFs associated with higher methane inhibition, whereas MIF 9.06_443.2317 and 15.00_487.2319 were associated with lower methane inhibition with no significant effect on in vitro organic matter digestibility of the feed. These MIFs could be used by plant breeders as potential markers to develop new M. oleifera varieties with high methane inhibition characteristics. However, further investigation on identifying the name, structure, and detailed biological activities of these bioactive metabolites needs to be carried out for future standardization, commercialization, and application as dietary methane mitigation additives.

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Candidate metabolites for methane mitigation in the forage legume biserrula

Cited by 6 publications

References 27 publications

An improved reference genome for Trifolium subterraneum L. provides insight into molecular diversity and intra-specific phylogeny

An improved reference genome for Trifolium subterraneum L. provides insight into molecular diversity and intra-specific phylogeny

Relationships between Agronomic Traits of Moringa Accessions and In Vitro Gas Production Characteristics of a Test Feed Incubated with or without Moringa Plant Leaf Extracts

Screening of Candidate Bioactive Secondary Plant Metabolite Ion-Features from Moringa oleifera Accessions Associated with High and Low Enteric Methane Inhibition from Ruminants

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