Candida yeast long chain fatty alcohol oxidase is a c-type haemoprotein and plays an important role in long chain fatty acid metabolism

Cheng, Qi; Sanglard, Dominique; Vanhanen, Sipo; Liu, Huan Ting; Bombelli, Paolo; Smith, Alison G.; Slabas, Antoni R.

doi:10.1016/j.bbalip.2005.06.006

Cited by 32 publications

(22 citation statements)

References 20 publications

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“…However, the latter proves to be not so straightforward, due to the presence of fatty alcohol oxidases (FAO) in S. bombicola (Hommel & Ratlegde, ). The fed fatty alcohols will be (partially) oxidized to the corresponding fatty aldehydes and further on to fatty acids in the suggested ω‐oxidation pathway (proven for Candida maltosa or Candida tropicalis by Cheng et al, ). Consequently, these fatty acids will be incorporated in the ΔatΔsble S. bombicola production pathway, giving rise to a mixture largely consisting of nsBola SLs (results not shown), which is unwanted.…”

Section: Resultsmentioning

confidence: 99%

From lab to market: An integrated bioprocess design approach for new‐to‐nature biosurfactants produced by Starmerella bombicola

Renterghem

Roelants

Baccile

et al. 2018

Biotech & Bioengineering

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Glycolipid microbial biosurfactants, such as sophorolipids (SLs), generate high industrial interest as 100% biobased alternatives for traditional surfactants. A well-known success story is the efficient SL producer Starmerella bombicola, which reaches titers well above 200 g/L. Recent engineering attempts have enabled the production of completely new types of molecules by S. bombicola, e.g. the bolaform SLs. Scale-up of bolaform SL production was performed at 150 L scale. The purified product was evaluated in detergent applications, as classic SLs are mostly applied in eco-friendly detergents. In this paper, we show that they can be used as green and non-irritant surfactants in for example (automatic) dishwashing applications. However, due to the presence of an ester function in the biosurfactant molecule a limited chemical stability at higher pH values (>6.5) was noticed, (therefore called 'non-symmetrical' (nsBola)) which, is a major drawback that will most likely inhibit market introduction. An integrated bioprocess design (IBPD) strategy was thus applied to resolve this issue. The strategy was to replace the fed fatty acids with fatty alcohols, to generate so-called "symmetrical bolaform (sBola) sophorosides (SSs)," containing two instead of one glycosidic bond. Next to a change in feeding strategy, the blocking of the fatty alcohols from metabolizing/oxidizing through the suggested ω-oxidation pathway was necessary. For the latter, two putative fatty alcohol oxidase genes (fao1 and fao2) were identified in the S. bombicola genome and deleted in the bolaform SL producing strain (ΔatΔsble). Shake flask experiments for these new strains (ΔatΔsbleΔfao1 and ΔatΔsbleΔfao2) were performed to evaluate if the fed fatty alcohols were directly implemented into the SL biosynthesis pathway. Indeed, sBola sophorosides (SSs) production up to 20 g/L was observed for the ΔatΔsbleΔfao1 strain. Unexpectedly, the ΔatΔsbleΔfao2 strain only produced minor amounts of sBola sophorosides (SSs), and mainly nsBola SLs (alike the parental ΔatΔsble strain). The sBola sophorosides (SSs) were purified and their symmetrical structure was confirmed by NMR. They were found to be significantly more stable at higher pH, opening up the application potential of the biosurfactant by enhancing its stability properties.

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Section: Resultsmentioning

confidence: 99%

From lab to market: An integrated bioprocess design approach for new‐to‐nature biosurfactants produced by Starmerella bombicola

Renterghem

Roelants

Baccile

et al. 2018

Biotech & Bioengineering

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“…It was also shown that a C. tropicalis FAOt ‐double mutant grew at a rate indistinguishable from the wild‐type on glucose and 1,11‐undecane‐dicarboxylic acid but could not grow on octadecane. They also verified that the FAOt ‐double mutant C. tropicalis NCYC470 strain could accumulate hexadecanol when grow on hexadecane as the sole carbon source (8).…”

Section: Introductionmentioning

confidence: 80%

Cloning and Characterization of Long‐Chain Fatty Alcohol Oxidase LjFAO1 in Lotus japonicus

Zhao

Lin

et al. 2008

Biotechnology Progress

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The Lotus japonicus EST database was searched against Arabidopsis thaliana AtFAO3, a fulllength cDNA that encodes a membrane-bound, flavin-containing, hydrogen peroxide generating, long-chain fatty alcohol oxidase. One EST fragment was detected, and the corresponding fulllength cDNA was obtained by screening a cDNA library of L. japonicus. The LjFAO1 genomic DNA was amplified by PCR, to give a product 3.6 kb in length. Comparison between the LjFAO1 cDNA and genomic DNA revealed that the LjFAO1 contains 3 exons and 2 introns. RT-PCR analysis showed that the LjFAO1 was expressed in the whole plant, with the highest expression level in the apex and the lowest expression level in the siliques. The LjFAO1 gene was downregulated by cold stress in both the apex and the cotelydon of the 8-day old seedlings, the first time that a long-chain alcohol oxidase has been shown to respond directly to stress. The full length cDNA and a C-terminal truncated version were overexpressed in Escherichia coli. The full length version of LjFAO1 exhibited long-chain fatty alcohol oxidase activity and was subsequently purified by Ni-NTA chromatography. The active LjFAO1 protein showed substrate specificities toward 1-dodecanol, 1-hexadecanol, and 1,16-hexadecanediol with K m values 59.6 ( 14.8 (µM), 40.9 ( 8.2 (µM) and 19.4 ( 1.5 (µM), respectively, suggesting apparent differences in substrate preferences with AtFAO3.

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“…Long‐chain fatty‐acid alcohol oxidase 3 (AtFAO3, At3G23410) is involved in the ω‐oxidation pathway of long‐chain fatty acid oxidation (Cheng et al ., ), and this pathway generates peroxide (Geissler and Hemmerich, ). We found that the fao mutant showed tolerance to three stresses (Figure ), possibly due to the disruption of peroxide generation.…”

Section: Discussionmentioning

confidence: 99%

The Alfin‐like homeodomain finger protein AL5 suppresses multiple negative factors to confer abiotic stress tolerance in Arabidopsis

et al. 2015

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SUMMARYPlant homeodomain (PHD) finger proteins affect processes of growth and development by changing transcription and reading epigenetic histone modifications, but their functions in abiotic stress responses remain largely unclear. Here we characterized seven Arabidopsis thaliana Alfin1-like PHD finger proteins (ALs) in terms of the responses to abiotic stresses. ALs localized to the nucleus and repressed transcription. Except AL6, all the ALs bound to G-rich elements. Mutations of the amino acids at positions 34 and 35 in AL6 caused loss of ability to bind to G-rich elements. Expression of the AL genes responded differentially to osmotic stress, salt, cold and abscisic acid treatments. AL5-over-expressing plants showed higher tolerance to salt, drought and freezing stress than Col-0. Consistently, al5 mutants showed reduced stress tolerance. We used ChIP-Seq assays to identify eight direct targets of AL5, and found that AL5 binds to the promoter regions of these genes. Knockout mutants of five of these target genes exhibited varying tolerances to stresses. These results indicate that AL5 inhibits multiple signaling pathways to confer stress tolerance. Our study sheds light on mechanisms of AL5-mediated signaling in abiotic stress responses, and provides tools for improvement of stress tolerance in crop plants.

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Candida yeast long chain fatty alcohol oxidase is a c-type haemoprotein and plays an important role in long chain fatty acid metabolism

Cited by 32 publications

References 20 publications

From lab to market: An integrated bioprocess design approach for new‐to‐nature biosurfactants produced by Starmerella bombicola

From lab to market: An integrated bioprocess design approach for new‐to‐nature biosurfactants produced by Starmerella bombicola

Cloning and Characterization of Long‐Chain Fatty Alcohol Oxidase LjFAO1 in Lotus japonicus

The Alfin‐like homeodomain finger protein AL5 suppresses multiple negative factors to confer abiotic stress tolerance in Arabidopsis

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