Candida-Associated Denture Stomatitis and Murine Models: What Is the Importance and Scientific Evidence?

Abstract

Considering the high prevalence and recurrence of Candida-associated denture stomatitis (CADS), in vivo studies in animal models are necessary before those in humans to evaluate new therapeutic strategies. This study aimed to review the literature on murine models of CADS induction using acrylic intraoral devices simulating dentures. Rats are recommended as experimental animals in these models as well as the adoption of a pasty diet. For maintenance in the proper position during the experiments, intraoral appliances must be obtained by individual impressions, using and retained exclusively by cementation on the molars. The region of interest for histopathological analysis was standardized as that corresponding to the area between the first molars. However, there is no consensus among the studies on the CADS induction rat models in relation to the Candida albicans inoculation and need for immunosuppression and/or administration of antibacterial drugs of animals. The greatest difficulty of the available models refers to maintaining the course of the lesion for a sufficient period to evaluate the effectiveness of the proposed treatment, considering the rapid and efficient murine immune response to candidal colonization. Therefore, future studies are necessary for the development of a robust and reproducible CADS model.

A Novel Approach of Administering Cranberry Extract Into 3D-Printed Denture Bases for the Prevention of Denture-Induced Stomatitis: An Observational Study

“…It disrupts Candida adhesion, hinders fungal growth, and reduces inflammation [ 11 ]. Studies by Sugio et al [ 16 ] and Anitha and Rajkumar [ 17 ] support its effectiveness.…”

A Novel Approach of Administering Cranberry Extract Into 3D-Printed Denture Bases for the Prevention of Denture-Induced Stomatitis: An Observational Study

“…It disrupts Candida adhesion, hinders fungal growth, and reduces inflammation [ 11 ]. Studies by Sugio et al [ 16 ] and Anitha and Rajkumar [ 17 ] support its effectiveness.…”

Gellan-Based Hydrogel as a Drug Delivery System for Caffeic Acid Phenethyl Ester in the Treatment of Oral Candida albicans Infections

“…Although CAPE showed potential to be used in the treatment of candidiasis, its low viscosity in a liquid solution can reduce the retention time in the oral cavity, and consequently, compromise the antifungal activity. In addition, factors such as salivary flow, tongue movements and swallowing can limit the retention of the compounds on the mucosa and prosthetic surfaces [ 8 ]. To overcome these limitations, an interesting alternative can be the use of a drug delivery system to provide a sustained release of the antifungal compound at the target site [ 43 , 44 ].…”

MIC Determination of Silver Nanoparticles on Nystatin-Resistant Candida albicans in Patients with Denture Stomatitis: An In Vitro Study

“…Candida albicans is the most reported DS-related microorganism (9,(28)(29)(30)(31). Unfortunately, many Candida biofilms are resistant to common antifungal drugs like fluconazole, nystatin, and amphotericin B, and frequent recurrences have been observed (8,32,33).…”

A Novel Approach of Administering Cranberry Extract Into 3D-Printed Denture Bases for the Prevention of Denture-Induced Stomatitis: An Observational Study

“…It disrupts Candida adhesion, hinders fungal growth, and reduces inflammation [ 11 ]. Studies by Sugio et al [ 16 ] and Anitha and Rajkumar [ 17 ] support its effectiveness.…”

Gellan-Based Hydrogel as a Drug Delivery System for Caffeic Acid Phenethyl Ester in the Treatment of Oral Candida albicans Infections

“…Although CAPE showed potential to be used in the treatment of candidiasis, its low viscosity in a liquid solution can reduce the retention time in the oral cavity, and consequently, compromise the antifungal activity. In addition, factors such as salivary flow, tongue movements and swallowing can limit the retention of the compounds on the mucosa and prosthetic surfaces [ 8 ]. To overcome these limitations, an interesting alternative can be the use of a drug delivery system to provide a sustained release of the antifungal compound at the target site [ 43 , 44 ].…”

MIC Determination of Silver Nanoparticles on Nystatin-Resistant Candida albicans in Patients with Denture Stomatitis: An In Vitro Study

“…Candida albicans is the most reported DS-related microorganism (9,(28)(29)(30)(31). Unfortunately, many Candida biofilms are resistant to common antifungal drugs like fluconazole, nystatin, and amphotericin B, and frequent recurrences have been observed (8,32,33).…”

A Novel Approach of Administering Cranberry Extract Into 3D-Printed Denture Bases for the Prevention of Denture-Induced Stomatitis: An Observational Study

“…It disrupts Candida adhesion, hinders fungal growth, and reduces inflammation [ 11 ]. Studies by Sugio et al [ 16 ] and Anitha and Rajkumar [ 17 ] support its effectiveness.…”

Gellan-Based Hydrogel as a Drug Delivery System for Caffeic Acid Phenethyl Ester in the Treatment of Oral Candida albicans Infections

“…Although CAPE showed potential to be used in the treatment of candidiasis, its low viscosity in a liquid solution can reduce the retention time in the oral cavity, and consequently, compromise the antifungal activity. In addition, factors such as salivary flow, tongue movements and swallowing can limit the retention of the compounds on the mucosa and prosthetic surfaces [ 8 ]. To overcome these limitations, an interesting alternative can be the use of a drug delivery system to provide a sustained release of the antifungal compound at the target site [ 43 , 44 ].…”

MIC Determination of Silver Nanoparticles on Nystatin-Resistant Candida albicans in Patients with Denture Stomatitis: An In Vitro Study

“…Candida albicans is the most reported DS-related microorganism (9,(28)(29)(30)(31). Unfortunately, many Candida biofilms are resistant to common antifungal drugs like fluconazole, nystatin, and amphotericin B, and frequent recurrences have been observed (8,32,33).…”