Can neutrophil responses in very low birth weight infants predict the organisms responsible for late-onset bacterial or fungal sepsis?

Sarkar, S; Bhagat, Indira; Hieber, Susan; Donn, Steven M.

doi:10.1038/sj.jp.7211554

Cited by 26 publications

(17 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Consequently, lower neutrophil and monocyte cell counts are observed in extremely low gestation preterm infants, which may play a more important role in increasing the risk of systemic infections in these infants (60, 61). Interestingly, neutropenia is often not observed during Candida sepsis in preterm neonates, in contrast to Gram-negative bacteria, which may indicate a more limited role for these cells once invasive infection has occurred (62). …”

Section: Innate Immune Responsesmentioning

confidence: 99%

Antifungal Immunological Defenses in Newborns

2017

View full text Add to dashboard Cite

Newborns are prone to fungal infections, largely due to Candida species. The immunological basis for this vulnerability is not yet fully understood. However, useful insights can be gained from the knowledge of the maturation of immune pathways during ontogeny, particularly when placed in context with how rare genetic mutations in humans predispose to fungal diseases. In this article, we review these most current data on immune functions in human newborns, highlighting pathways most relevant to the response to Candida. While discussing these data, we propose a framework of why deficiencies in these pathways make newborns particularly vulnerable to this opportunistic pathogen.

show abstract

Section: Innate Immune Responsesmentioning

confidence: 99%

Antifungal Immunological Defenses in Newborns

2017

View full text Add to dashboard Cite

show abstract

“…In neonates, prolonged duration of antibiotic therapy is associated with increased risk of sepsis after 4 days of life, known as late-onset sepsis (LOS) 2 , a disorder critically controlled by neutrophils 3 , but a role for the microbiota in regulating neutrophil behavior in the neonate has not been described. We exposed pregnant mouse dams to antibiotics in drinking water to limit transfer of maternal microbes to the neonates.…”

mentioning

confidence: 99%

“…Neutropenia is an important risk factor in fatal neonatal sepsis 3 . In order to test whether antibiotic exposure modified host defense against infection, neonatal mice (postnatal day 3 or 5 or 7 or 14 old) were exposed to the combination of 5 antibiotics or the more clinically relevant combination of ampicillin, gentamicin and vancomycin through their Dams beginning 5 days before birth and continuing until day 14.…”

mentioning

confidence: 99%

The microbiota regulates neutrophil homeostasis and host resistance to Escherichia coli K1 sepsis in neonatal mice

Deshmukh

Liu

Menkiti

et al. 2014

Nat Med

476

436

View full text Add to dashboard Cite

Acquisition of microbes by the neonate, which begins immediately during birth, is influenced by gestational age and mother’s microbiota and modified by exposure to antibiotics1. In neonates, prolonged duration of antibiotic therapy is associated with increased risk of sepsis after 4 days of life, known as late-onset sepsis (LOS)2, a disorder critically controlled by neutrophils3, but a role for the microbiota in regulating neutrophil behavior in the neonate has not been described. We exposed pregnant mouse dams to antibiotics in drinking water to limit transfer of maternal microbes to the neonates. Antibiotic exposure of dams decreased the total number of microbes in the intestine, altered the structure of intestinal microbiota and changed the pattern of microbial colonization. These changes were associated with decreased numbers of circulating and bone marrow neutrophils and granulocyte/macrophage restricted progenitor cells in the bone marrow. Antibiotic-exposure of dams attenuated the postnatal granulocytosis by reducing the number of interleukin (IL) 17-producing cells in intestine and consequent production of granulocyte colony stimulating factor (G-CSF). Relative granulocytopenia contributed to increased susceptibility of antibiotic-exposed neonatal mice to Escherichia coli K1 and Klebsiella pneumoniae sepsis, which could be partially reversed by administration of G-CSF. Restoration of normal microbiota, through TLR4- and MYD88-dependent mechanism, induced accumulation of IL17-producing type 3 innate lymphoid cells (ILC) in the intestine, promoted granulocytosis, and restored the IL17-dependent resistance to sepsis. Specific depletion of ILCs prevented the IL17- and G-CSF-dependent granulocytosis and resistance to sepsis. These data support a role for the intestinal microbiota in regulation of granulocytosis and host resistance to sepsis in the neonates.

show abstract

“…Costa et al [14] in Portugal, Khashu [15] in Canada, Sarkar et al [16] in the United State of America, and Richards et al [17] in Colombia reported similar findings, what demonstrates that these organisms are prevalent agents of LOS in different countries and continents. P. aeruginosa was the most frequent agent of LOS, 9.1%, among gram-negative organisms.…”

Section: Discussionmentioning

confidence: 75%

Sepsis-Related Mortality of Very Low Birth Weight Brazilian Infants: The Role ofPseudomonas aeruginosa

Pereira

Cardoso²,

Figuexeds

et al. 2009

International Journal of Pediatrics

View full text Add to dashboard Cite

The aim of this study is to identify risk factors for sepsis-related mortality in low birth weight (<1500 g) infants. We performed retrospective cohort study to investigate risk factors for sepsis-related mortality in all neonates birth weight <1500 g admitted to Level III neonatal intensive care unit, Brazil, April 2001/September 2004. Of the 203 cases, 71 (35%) had sepsis. Of those, gram-positive was identified in 52/87 blood cultures (59.8%), the most common Coagulase-negative Staphylococcus (31/87; 35.5%). Gram-negative was present in 29 of the 87 positive blood cultures (33.3%), with Pseudomonas aeruginosa (8/87; 9.1%), the most frequent agent. Overall 21 of 71 infants with sepsis (29.6%) died. Risk factors for sepsis-related mortality were gestational age ≤28 weeks, birth weight ≤1000 g (9.6 times more often than birth weight >1000 g), five-minute Apgar ≤7, gram-negative sepsis, mechanical ventilation (6.7 times higher than no use), and intravascular catheter. Sepsis-related mortality was due, mainly, to Pseudomonas aeruginosa; birth weight ≤1000 g and mechanical ventilation were strong sepsis-related mortality predictors.

show abstract

Can neutrophil responses in very low birth weight infants predict the organisms responsible for late-onset bacterial or fungal sepsis?

Cited by 26 publications

References 18 publications

Antifungal Immunological Defenses in Newborns

Antifungal Immunological Defenses in Newborns

The microbiota regulates neutrophil homeostasis and host resistance to Escherichia coli K1 sepsis in neonatal mice

Sepsis-Related Mortality of Very Low Birth Weight Brazilian Infants: The Role ofPseudomonas aeruginosa

Contact Info

Product

Resources

About