Can intracytoplasmic morphologically selected sperm injection be used to select normal-sized sperm heads in infertile patients with macrocephalic sperm head syndrome?

Chelli, Mohamed; Albert, Martine; Ray, Pierre F.; Guthauser, Bruno; Izard, V.; Hammoud, Ibrahim; Selva, J.; Vialard, François

doi:10.1016/j.fertnstert.2008.10.059

Cited by 39 publications

(30 citation statements)

References 18 publications

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“…Even though Perdrix et al [27] observed some aneuploidy rate differences between spermatozoa with large vacuoles and whole sperm from patients with a normal karyotype, other authors failed to observe this association [8,32], for a review, see [24]. Similar results were also found for infertile patients with macrocephalic head syndrome, in whom no euploid spermatozoa had been retrieved after ICSI-like selection or MSOME [11]. Only diploid spermatozoa with an enlarged head could be selected by MSOME.…”

Section: Discussionmentioning

confidence: 81%

“…In a glass-bottomed dish (the WillCo-dish from WillCo Wells BV, The Netherlands), 5-10 μl of the sperm fraction obtained after density gradient selection were diluted into a 4 μl microdroplet of 10 % polyvinylpyrrolidone in flushing medium (FertiPro N.V., Belgium). Spermatozoa were then selected at an ICSI-like magnification (×200) or an MSOME-like magnification with a Nikon inverted microscope equipped with an ×100 objective and Nomarski contrast, as previously described [11]. We selected normal spermatozoa according to the WHO criteria (WHO [34]).…”

Section: Semen Preparation and Parameters For Whole Spermmentioning

confidence: 99%

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High-magnification sperm selection does not decrease the aneuploidy rate in patients who are heterozygous for reciprocal translocations

Chelli

Ferfouri

Boitrelle

et al. 2013

J Assist Reprod Genet

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Problem This study sought to evaluate the value of motile sperm organelle morphology examination (MSOME) for selecting euploid spermatozoa in six patients who were heterozygous for a reciprocal translocation. Method of study We used sperm fluorescence in situ hybridization (FISH) to screen for aneuploidy of the chromosomes involved in the translocations and a putative interchromosomal effect (ICE) for chromosomes 18, X and Y. This procedure was performed on (i) whole sperm (i.e. no selection) and on normal spermatozoa selected (ii) at a magnification typically used for intracytoplasmic sperm injection (ICSI), referred to as "ICSIlike", and (iii) with MSOME. Results The balanced translocation rates did not differ significantly (p=0.81) when comparing whole sperm (57.2 %) with spermatozoa after ICSI-like selection (56.3 %) or after MSOME (53.7 %). Similarly, the aneuploidy rates for ICEs did not differ significantly (p=0.14) when comparing whole sperm (1.9 %), ICSI-selected spermatozoa (3.4 %) and MSOME-selected spermatozoa (1.0 %). Conclusion For patients who are heterozygous for reciprocal translocations, MSOME does not improve the selection of euploid spermatozoa.

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Section: Discussionmentioning

confidence: 81%

Section: Semen Preparation and Parameters For Whole Spermmentioning

confidence: 99%

High-magnification sperm selection does not decrease the aneuploidy rate in patients who are heterozygous for reciprocal translocations

Chelli

Ferfouri

Boitrelle

et al. 2013

J Assist Reprod Genet

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“…Low fertilization and pregnancy rates are achieved with ICSI, which may be because of the high incidence of chromosomal aberrations in the ejaculated megalo-headed spermatozoa [58].…”

Section: Large-headed Spermatozoamentioning

confidence: 99%

Clinical significance of the low normal sperm morphology value as proposed in the fifth edition of the WHO Laboratory Manual for the Examination and Processing of Human Semen

Menkveld¹

2010

Asian J Androl

175

109

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The very low cut-off value for sperm morphology of 4% morphologically normal spermatozoa, as proposed in the new edition of the World Health Organization (WHO) manual on semen analysis, is in agreement with recently published values and reflects the trend of a decline in reported mean values for normal sperm morphology. The reduced value for morphologically normal spermatozoa over the years may be due to several factors. The first is the introduction of strict criteria for the evaluation of sperm morphology. Other reasons may include the introduction of additional criteria for sperm morphology abnormalities and the suggested decrease in semen parameters because of increasing negative environmental influences. Although on its own the newly proposed very low normal value may not provide the strong predictive value for a males' fertility potential, as originally reported for sperm morphology evaluated according to strict criteria, a good predictive value can still be obtained if the holistic, strict approach for sperm morphology evaluation is followed together with additional sperm morphology parameters now available, because certain morphology patterns and sperm abnormalities are now known to be of strong prognostic value. In addition, better international standardization of the technical methodology, consensus on the interpretation of sperm morphology evaluation criteria and standardized international external quality control (EQC) schemes, are of utmost importance to maintain the good predictive value of sperm morphology.

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“…Macrocephalic spermatozoa are unable to enter into the oocyte, and intra-cytoplasmic sperm injection (ICSI), although more effective in obtaining fertilization, is risky because of the relevant chance to generate a genetically altered offspring. Some new techniques, such as intracytoplasmic morphologically selected sperm injection or polarized light microscopy anal-ysis of spermatozoa ( Magli et al, 2012;Wilding et al, 2011) could possibly help in eventually selecting rare normal sper-matozoa in these patients, but their real effectiveness in identifying euploid spermatozoa is this specific situation is uncertain ( Celik-Ozenci et al, 2004;Chelli et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Sperm macrocephaly syndrome in a patient without AURKC mutations and with a history of recurrent miscarriage

Molinari¹,

Mirabelli

Raimondo

et al. 2013

Reproductive BioMedicine Online

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Can intracytoplasmic morphologically selected sperm injection be used to select normal-sized sperm heads in infertile patients with macrocephalic sperm head syndrome?

Cited by 39 publications

References 18 publications

High-magnification sperm selection does not decrease the aneuploidy rate in patients who are heterozygous for reciprocal translocations

High-magnification sperm selection does not decrease the aneuploidy rate in patients who are heterozygous for reciprocal translocations

Clinical significance of the low normal sperm morphology value as proposed in the fifth edition of the WHO Laboratory Manual for the Examination and Processing of Human Semen

Sperm macrocephaly syndrome in a patient without AURKC mutations and with a history of recurrent miscarriage

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