Can fracture healing be accelerated by serum transfer in head trauma cases? An experimental head trauma model in rats

Abstract

Objectives: In this study, we aimed to investigate whether the positive union effect caused by head trauma could be transferred between individuals. Materials and methods: Seventy-two male rats with an average weight of 375 g were used in this study and divided into four groups including 18 in each group. Group 1 consisted of serum donor rats that were exposed to head trauma, while Group 2 consisted of study rats with long bone fractures that were given the serum obtained from the rats in Group 1, Group 3 included control rats with isolated long bone fractures, and Group 4 included control rats with both head trauma and long bone fractures. For radiological evaluation, the ratio of the width of the callus to the width of the neighboring diaphysis was considered as the callus-to- diaphyseal ratio in the study and control groups. Histopathological and radiological evaluations was made on Days 10, 20, and 30. Results: In evaluation of the radiological data regarding the callus- to-diaphyseal ratio, Group 3 was found to have significantly lower radiological values than Group 4 on Day 10 (p=0.006). Group 2 had significantly higher values than Group 3 (p=0.02). On Day 20, Group 2 exhibited significantly higher radiological values than Group 3 (p=0.004), but lower than Group 4 (p=0.032). As for Day 30, Group 2 exhibited significantly higher radiological values than Group 3, but lower than Group 4 (p=0.001). In the evaluation of the Huo scores obtained for histopathological evaluation, there was no significant difference among the groups on Days 10, 20, and 30 (p=0.295, p=0.569, and p=0.729, respectively). Conclusion: Our study results suggest that the osteoinductive effect after head trauma can be transmitted between individuals by means of serum transfer.

Investigation of the effects of inflammatory and metabolic factors on fracture union in head trauma and long bone fractures

“…The literature suggests that the sera obtained from HT cases in in vitro cell culture media positively affects osteoblastic cell proliferation [17]. Sari et al [40] stated that fracture union could be induced radiologically and histopathologically by in vivo serum transfer. The importance of inflammatory and antiinflammatory cytokine balance in the bone union process has been shown at the molecular level.…”