2013
DOI: 10.1186/1471-2156-14-20
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Abstract: BackgroundThe megadiverse Neotropical freshwater ichthyofauna is the richest in the world with approximately 6,000 recognized species. Interestingly, they are distributed among only 17 orders, and almost 80% of them belong to only three orders: Characiformes, Siluriformes and Perciformes. Moreover, evidence based on molecular data has shown that most of the diversification of the Neotropical ichthyofauna occurred recently. These characteristics make the taxonomy and identification of this fauna a great challen… Show more

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Cited by 242 publications
(255 citation statements)
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References 75 publications
(89 reference statements)
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“…All specimens of K. moenkhausii from the three among the three hydrographic basins and the haplotypes of both genes were shared among the three hydrographic basins revealing a complete lack of population structure. The genetic distance from congeners ranged from 8.6% to 11.0 and 9.3% to 11.0% for COI and CytB genes, respectively: values consistent with those found in other DNA barcoding surveys that showed average genetic distances between congeners ranging from 7% to 11% in more than 90% of comparisons [28,30,31,47,48]. Similarly, these other studies report genetic divergences within species of about 0.5%, a similar value to that found in the present survey.…”
Section: Resultssupporting
confidence: 80%
“…All specimens of K. moenkhausii from the three among the three hydrographic basins and the haplotypes of both genes were shared among the three hydrographic basins revealing a complete lack of population structure. The genetic distance from congeners ranged from 8.6% to 11.0 and 9.3% to 11.0% for COI and CytB genes, respectively: values consistent with those found in other DNA barcoding surveys that showed average genetic distances between congeners ranging from 7% to 11% in more than 90% of comparisons [28,30,31,47,48]. Similarly, these other studies report genetic divergences within species of about 0.5%, a similar value to that found in the present survey.…”
Section: Resultssupporting
confidence: 80%
“…This homology test means that all strains belong to Osphronemus goramy Lacepede, 1801. This placement is fulfilled the requirement of similarity among individuals as notes by Pereira et al (2013) that two or more individuals can be referred to as a single species when they have sequences similarities between 97% and 100%. Species delineation of all strains to a single species was supported by the low level of K-2P genetic distances among strains (from 0.0% to 1.1%).…”
Section: Resultsmentioning
confidence: 99%
“…In specific groups, such as the Neotropical fish, studies have used the barcoding threshold (approximately 2%) proposed by Ward (2009) to delimit a wide-range of species (Rosso et al, 2012;Bellafronte et al,. 2013;Pereira et al, 2013;Gomes et al, 2015;Shimabukuro-Dias et al, 2016;Díaz et al, 2016). Nonetheless, given the great diversity of 6 e160147 [6] species, particularly related to life histories and complexity of environment, a fixed threshold value can under-or overestimate the correct number of MOTUs (Brown et al, 2012).…”
Section: Discussionmentioning
confidence: 99%