Can biological nitrification inhibition (BNI) genes from perennial Leymus racemosus (Triticeae) combat nitrification in wheat farming?

Subbarao, G. V.; Ban, Tomohiro; Kishii, M.; Ito, Osamu; Samejima, Hiroaki; Wang, H. Y.; Pearse, Stuart J.; Gopalakrishnan, Subramaniam; Nakahara, Kazuhiko; Hossain, Alamgir; Tsujimoto, Hisashi; Berry, Wade L.

doi:10.1007/s11104-007-9360-z

Cited by 103 publications

(91 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…(24). Also, intro- ducing high BNI capacity from wild wheat (Leymus racemosus) into cultivated wheat could be an option in the foreseeable future (26,48). A fundamental shift toward NH 4 ϩ -dominated crop nutrition can be achieved by using crops and pastures that have high BNI capacity or integrating annual crop production with a high BNI-capacity forage component, resulting in low-nitrifying agronomic production systems, benefiting both agriculture and the environment.…”

Section: Discussionmentioning

confidence: 99%

“…Using this methodology, we were able to show that certain plants release nitrification inhibitors from their roots (23)(24)(25)(26). Such BNI capacity appears to be relatively widespread among tropical pasture plants, with Brachiaria spp.…”

mentioning

confidence: 99%

“…showing the highest capacity among the pasture grasses tested (24). The potential for high BNI capacity also exists in wild wheat (26). A pasture grass, Brachiaria humidicola (Rendle) Schweick, native to tropical Africa and grown extensively in tropical South American grasslands, releases substantial amounts of BNIs from its roots, ranging from 17 to 50 ATU per gram of root dry weight per day (23,24).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Evidence for biological nitrification inhibition inBrachiariapastures

Subbarao

Nakahara²,

Hurtado³

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

410

393

View full text Add to dashboard Cite

Nitrification, a key process in the global nitrogen cycle that generates nitrate through microbial activity, may enhance losses of fertilizer nitrogen by leaching and denitrification. Certain plants can suppress soil-nitrification by releasing inhibitors from roots, a phenomenon termed biological nitrification inhibition (BNI). Here, we report the discovery of an effective nitrification inhibitor in the root-exudates of the tropical forage grass Brachiaria humidicola (Rendle) Schweick. Named ''brachialactone,'' this inhibitor is a recently discovered cyclic diterpene with a unique 5-8-5-membered ring system and a ␥-lactone ring. It contributed 60 -90% of the inhibitory activity released from the roots of this tropical grass. Unlike nitrapyrin (a synthetic nitrification inhibitor), which affects only the ammonia monooxygenase (AMO) pathway, brachialactone appears to block both AMO and hydroxylamine oxidoreductase enzymatic pathways in Nitrosomonas. global warming ͉ nitrogen pollution ͉ nitrous oxide emissions ͉ root exudation ͉ climate change M ost modern agricultural systems are based on large inputs of inorganic nitrogen (N), with ammonium (NH 4 ϩ ) being the primary N source (1, 2). Also, current crop management practices result in the development of highly nitrifying soil environments (3, 4). Nitrification results in the transformation of the relatively immobile NH 4 ϩ to highly mobile nitrate (NO 3 Ϫ ), making inorganic N susceptible to losses through leaching of NO 3 Ϫ and/or gaseous N emissions, potentially initiating a cascade of environmental and health problems (1, 2, 5, 6). Nitrous oxide (N 2 O) is one of the three major biogenic greenhouse gases contributing to global warming, produced primarily from denitrification processes in agricultural systems (5, 7). Also, assimilation of NO 3 Ϫ by plants can result in further N 2 O emissions directly from plant canopies (8). The low agronomic N-use efficiency (NUE) found in many agricultural systems is largely the result of N losses associated with nitrification (i.e., N losses from NO 3 Ϫ leaching and denitrification) (9-11). Most plants have the ability to assimilate both NH 4 ϩ and NO 3 Ϫ (12); therefore, nitrification does not need to be a dominant process in the N cycle for efficient N use.Nitrification is low in some forest and grassland soils (13-17). Since the early 1960s, some tropical grasses have been suspected of having the capacity to inhibit nitrification (18-21). However, this concept remained controversial due to the lack of direct evidence showing such inhibitory effects or the identification of specific inhibitors (22).We adopted a very sensitive bioassay using a recombinant luminescent Nitrosomonas europaea to detect biological nitrification inhibition (BNI) in plant-soil systems with the inhibitory activity of roots expressed in allylthiourea units (ATU) (23). Using this methodology, we were able to show that certain plants release nitrification inhibitors from their roots (23-26). Such BNI capacity appears to be relatively widespread among...

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Evidence for biological nitrification inhibition inBrachiariapastures

Subbarao

Nakahara²,

Hurtado³

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

410

393

View full text Add to dashboard Cite

show abstract

“…The BNI concept The natural ability of some plants to produce and release nitrification inhibitors from roots to suppress nitrifier activity in soils is termed 'biological nitrification inhibition (BNI)' (for details see Figure 3) (Subbarao et al, 2006a(Subbarao et al, , 2006b(Subbarao et al, , 2007a(Subbarao et al, , 2007b(Subbarao et al, , 2007c(Subbarao et al, , 2009a(Subbarao et al, , 2009b(Subbarao et al, and 2013b. As nitrification is the most important process determining the N-cycling efficiency (i.e.…”

Section: Biological Nitrification Inhibition (Bni)mentioning

confidence: 99%

“…Preliminary evaluation suggested a lack of significant BNI capacity in the cultivated wheat. Subsequent evaluation of wild wheats indicated that roots of Leymus racemosus, a wild relative of wheat, possess high-BNI capacity (Subbarao et al, 2007c). The rate of suppression by L. racemosus was effective in reducing soil nitrification (Subbarao et al, 2007c).…”

Section: Biological Nitrification Inhibition (Bni)mentioning

confidence: 99%

Potential for biological nitrification inhibition to reduce nitrification and N2O emissions in pasture crop–livestock systems

Subbarao

Rao

Nakahara

et al. 2013

Animal

Self Cite

View full text Add to dashboard Cite

Agriculture and livestock production systems are two major emitters of greenhouse gases. Methane with a GWP (global warming potential) of 21, and nitrous oxide (N 2 O) with a GWP of 300, are largely emitted from animal production agriculture, where livestock production is based on pasture and feed grains. The principal biological processes involved in N 2 O emissions are nitrification and denitrification. Biological nitrification inhibition (BNI) is the natural ability of certain plant species to release nitrification inhibitors from their roots that suppress nitrifier activity, thus reducing soil nitrification and N 2 O emission. Recent methodological developments (e.g. bioluminescence assay to detect BNIs in plant root systems) have led to significant advances in our ability to quantify and characterize the BNI function. Synthesis and release of BNIs from plants is a highly regulated process triggered by the presence of NH 4 1 in the rhizosphere, which results in the inhibitor being released precisely where the majority of the soil-nitrifier population resides. Among the tropical pasture grasses, the BNI function is strongest (i.e. BNI capacity) in Brachiaria sp. Some feed-grain crops such as sorghum also have significant BNI capacity present in their root systems. The chemical identity of some of these BNIs has now been established, and their mode of inhibitory action on Nitrosomonas has been characterized. The ability of the BNI function in Brachiaria pastures to suppress N 2 O emissions and soil nitrification potential has been demonstrated; however, its potential role in controlling N 2 O emissions in agro-pastoral systems is under investigation. Here we present the current status of our understanding on how the BNI functions in Brachiaria pastures and feed-grain crops such as sorghum can be exploited both genetically and, from a production system's perspective, to develop low-nitrifying and low N 2 O-emitting production systems that would be economically profitable and ecologically sustainable.

show abstract

An Overview of Nutrient Use Efficiency and Strategies for Crop Improvement

Hawkesford

2011

The Molecular and Physiological Basis of Nutrient Use Efficiency in Crops

View full text Add to dashboard Cite

Can biological nitrification inhibition (BNI) genes from perennial Leymus racemosus (Triticeae) combat nitrification in wheat farming?

Cited by 103 publications

References 28 publications

Evidence for biological nitrification inhibition inBrachiariapastures

Evidence for biological nitrification inhibition inBrachiariapastures

Potential for biological nitrification inhibition to reduce nitrification and N2O emissions in pasture crop–livestock systems

An Overview of Nutrient Use Efficiency and Strategies for Crop Improvement

Contact Info

Product

Resources

About