Campylobacter infections in fattening pigs; excretion pattern and genetic diversity

Abstract

The excretion of campylobacter by eight individually housed fattening pigs was monitored during 15 weeks. Rectal faeces samples were collected six times from these pigs and twice from their mothers (seven sows). Campylobacter was cultured from these samples on Preston medium. In some pigs, samples positive for campylobacter alternated with negative samples. Campylobacter was detected in at least four of the six samples collected per fattening pig. The average campylobacter count per sampling showed a decreasing trend (P < 0·001). of the seven sows, six were shown to excrete campylobacter. Campylobacter isolates of pigs and sows were typed using the Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction (ERIC‐PCR); 28 different campylobacter types were distinguished. Up to five different types were isolated from single faeces samples. Individual porkers could harbour up to eight types during their fattening period. The three types most frequently isolated from the fattening pigs were also present in the sows.

(CGG) 4 -Based PCR as a Novel Tool for Discrimination of Uropathogenic Escherichia coli Strains: Comparison with Enterobacterial Repetitive Intergenic Consensus-PCR

“…The discriminative power of the REP-PCR group, including ERIC-PCR, has been determined to be sufficient for smallscale epidemiological studies involving E. coli strains. It possesses a higher discriminatory ability for many microorganisms than do other quick-typing techniques, leading to its increased frequency of use (1,6,33,41). However, in the literature, the conclusion that ERIC-PCR possesses high discriminatory power is independent of any examination of reproducibility, which significantly impacts the determination of strain differences (19,23).…”

Campylobacter coliin Swine Production: Antimicrobial Resistance Mechanisms and Molecular Epidemiology

“…These findings imply that both genotyping methods are very good, and the use of both methods would ideally be a preferred way for tracking clones. It should also be emphasized that the outcome of PFGE typing is susceptible to changes in the chromosome and inter-and intragenomic recombinations, making this method unsuitable for studying the epidemiology of pathogens with hyperplastic genome including Campylobacter (18,34,47,48). Analysis of clusters generated by the two methods revealed the high level of genotypic diversity present in C. coli.…”

Discrimination of Enterobacterial Repetitive Intergenic Consensus PCR Types of Campylobacter coli and Campylobacter jejuni by Fourier Transform Infrared Spectroscopy

“…Sixteen strains were isolated from animal feces (pigs and chickens) and one from human feces (a clinical case). The strains were isolated, identified, and DNA fingerprinted by means of the ERIC-PCR technique (30,49). All strains were kept frozen (Ϫ80°C) in brain heart infusion (BHI) broth containing 20% glycerol during the experiments.…”

“…The reproducibility and discriminatory power of the ERIC-PCR technique in the typing of thermophilic Campylobacter spp. favors its application in epidemiological studies (6,42,48,49).…”